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Title:
REAGENT FOR DETECTION OF BACTERIUM ALICYCLOBACILLUS ACIDOTERRESTRIS, CULTURE MEDIUM FOR THE DETECTION AND METHOD FOR THE DETECTION
Document Type and Number:
WIPO Patent Application WO/2006/112461
Kind Code:
A1
Abstract:
[PROBLEMS] To provide a reagent for the detection of a bacterium Alicyclobacillus acidoterrestris which can detect Alicyclobacillus acidoterrestris rapidly and can distinguish Alicyclobacillus acidoterrestris from Alicyclobacillus acidocaldarius to confirm the presence of Alicyclobacillus acidoterrestris readily, a culture medium for use in the detection, and a method for the detection. [MEANS FOR SOLVING PROBLEMS] A sample containing an extracted fruit juice is smeared onto the surface of an Alicyclobacillus acidoterrestris detection culture medium containing 2,3,5-triphenyl-2H-tetrazolium chloride, and the culture medium is then incubated at a temperature ranging from 40&ring C to 50&ring C. The presence of a bacterium Alicyclobacillus acidoterrestris can be detected on the basis of the presence of red color development caused by the colonies formed on the culture medium.

Inventors:
OGAWA HIROYUKI (JP)
MIYASHITA MITSUYOSHI (JP)
Application Number:
PCT/JP2006/308153
Publication Date:
October 26, 2006
Filing Date:
April 18, 2006
Export Citation:
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Assignee:
MICROBIO KABUSHIKI KAISHA (JP)
OGAWA HIROYUKI (JP)
MIYASHITA MITSUYOSHI (JP)
International Classes:
C12Q1/04
Foreign References:
JP2004041104A2004-02-12
JPH0678793A1994-03-22
Other References:
KITAMURA K. ET AL.: "Teishoku Hannoshiki ni yoru Ekitai Baichi o Mochiita Alicyclobacillus-Zoku Saikin no Jinsoku Kenshutsu Hoho", NIPPON NOGEI KAGAKU KAISHI, vol. 75, 2001, pages 267, 3M1P3, XP003006488
Attorney, Agent or Firm:
Suda, Atsushi (Nishikicho 1-chome Aoba-k, Sendai-shi Miyagi 12, JP)
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