Title:
REAGENT FOR MEASURING TYROSINE KINASE ACTIVITY, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/058178
Kind Code:
A1
Abstract:
The present invention provides a reagent that is for measuring an epidermal growth factor receptor tyrosine kinase activity and that contains: a modified polypeptide to which an acceptor and a donor inducing Förster resonance energy transfer are bound, and which includes CrkL, a CrkL fragment retaining an SH2 domain and a portion subjected to phosphorylation by a tyrosine kinase, or a CrkL mutant that has an amino acid sequence having an identity of 90% or more with respect to an amino acid sequence of CrkL or the CrkL fragment and that becomes a substrate for a tyrosine kinase; or a nucleic acid encoding the modified polypeptide. The present invention also provides a method for assessing susceptibility of non-small cell lung cancer cells to a tyrosine kinase inhibitor, a method for preparing non-small cell lung cancer cells that are resistant to a tyrosine kinase inhibitor, and a method for assessing an epidermal growth factor receptor tyrosine kinase activity of non-small cell lung cancer cells, wherein said methods all use the reagent.
Inventors:
OHBA YUSUKE (JP)
AKITA HIROTOSHI (JP)
AKITA HIROTOSHI (JP)
Application Number:
PCT/JP2023/033214
Publication Date:
March 21, 2024
Filing Date:
September 12, 2023
Export Citation:
Assignee:
HILO CO LTD (JP)
International Classes:
C12Q1/48; C12Q1/02; G01N33/50; C07K14/435; C07K14/47; C07K19/00; C12N15/12; C12N15/62
Domestic Patent References:
| WO2014168143A1 | 2014-10-16 |
Foreign References:
| JP2009278942A | 2009-12-03 |
Other References:
AN RONG, WANG YISONG, VOELLER DONNA, GOWER ARJAN, KIM IN-KYU, ZHANG YU-WEN, GIACCONE GIUSEPPE: "CRKL mediates EML4-ALK signaling and is a potential therapeutic target for ALK-rearranged lung adenocarcinoma", ONCOTARGET, IMPACT JOURNALS LLC, UNITED STATES, vol. 7, no. 20, 17 May 2016 (2016-05-17), United States , pages 29199 - 29210, XP093146102, ISSN: 1949-2553, DOI: 10.18632/oncotarget.8638
Attorney, Agent or Firm:
IP-ASSIST PATENT PARTNERS (JP)
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