Title:
REAGENT AND METHOD FOR DETECTING TARGET SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2023/191079
Kind Code:
A1
Abstract:
The present invention provides a practicable assay system using a specific aptamer, particularly a reagent and a method for detecting a homogenous target substance. A nucleic acid molecule to be used in this detection reagent or this detection method comprises a first aptamer that acts on porphyrin exhibiting a peroxidase activity to increase the peroxidase activity and a second aptamer which is linked to the first aptamer and is capable of bonding to a target substance.
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Inventors:
IKEBUKURO KAZUNORI (JP)
ASANO RYUTARO (JP)
MIURA DAIMEI (JP)
YOSHINO TOMOKO (JP)
ASANO RYUTARO (JP)
MIURA DAIMEI (JP)
YOSHINO TOMOKO (JP)
Application Number:
PCT/JP2023/013644
Publication Date:
October 05, 2023
Filing Date:
March 31, 2023
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION TOKYO UNIV OF AGRICULTURE AND TECHNOLOGY (JP)
International Classes:
C12Q1/68; C12N15/115; C12Q1/26; C12Q1/28
Domestic Patent References:
| WO2017188426A1 | 2017-11-02 | |||
| WO2016168656A1 | 2016-10-20 | |||
| WO2022038521A1 | 2022-02-24 |
Foreign References:
| JP2003028874A | 2003-01-29 | |||
| JP2006075103A | 2006-03-23 | |||
| KR20170099611A | 2017-09-01 |
Other References:
DAIMEI MIURA, WAKANA HAYASHI, KENSUKE HIRANO, IKKEI SASAKI, KAORI TSUKAKOSHI, WAKAKO TSUGAWA, KOJI SODE, KAZUNORI IKEBUKURO, RYUTA: "P-24: Rapid and convenient viral detection based on sequential enzymatic reactions induced by antibody-enzyme complex and aptamer binding", PROCEEDINGS OF THE ENZYME ENGINEERING RESEARCH SOCIETY SYMPOSIUM, SEPTEMBER 30 - OCTOBER 1, 2022, JAPANESE SOCIETY OF ENZYME ENGINEERING; SOCIETY OF BIOCATALYSIS JAPAN, JP, 1 September 2022 (2022-09-01) - 1 October 2022 (2022-10-01), JP, pages 62, XP009550194
TSUKAKOSHI KAORI, YAMAGISHI YASUKO, KANAZASHI MANA, NAKAMA KENTA, OSHIKAWA DAIKI, SAVORY NASA, MATSUGAMI AKIMASA, HAYASHI FUMIAKI,: "G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 49, no. 11, 21 June 2021 (2021-06-21), GB , pages 6069 - 6081, XP093097367, ISSN: 0305-1048, DOI: 10.1093/nar/gkab388
XIAOHONG CHENG, XIANGJUN LIU, TAO BING, ZEHUI CAO, DIHUA SHANGGUAN: "General Peroxidase Activity of G-Quadruplex−Hemin Complexes and Its Application in Ligand Screening", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, vol. 48, no. 33, 25 August 2009 (2009-08-25), pages 7817 - 7823, XP055140326, ISSN: 00062960, DOI: 10.1021/bi9006786
DE‐MING KONG; LI‐LI CAI; JUN‐HONG GUO; JING WU; HAN‐XI SHEN: "Characterization of the G‐quadruplex structure of a catalytic DNA with peroxidase activity", BIOPOLYMERS, JOHN WILEY, HOBOKEN, USA, vol. 91, no. 5, 9 January 2009 (2009-01-09), Hoboken, USA, pages 331 - 339, XP071112736, ISSN: 0006-3525, DOI: 10.1002/bip.21135
YOSHINO TOMOKO, MAEDA YOSHIAKI, MATSUNAGA TADASHI: "Bioengineering of Bacterial Magnetic Particles and their Applications in Biotechnology", RECENT PATENTS ON BIOTECHNOLOGY, BENTHAM SCIENCE PUBLISHERS, NL, vol. 4, no. 3, 1 November 2010 (2010-11-01), NL , pages 214 - 225, XP093097370, ISSN: 1872-2083, DOI: 10.2174/187220810793611455
TSUKAKOSHI KAORI, YAMAGISHI YASUKO, KANAZASHI MANA, NAKAMA KENTA, OSHIKAWA DAIKI, SAVORY NASA, MATSUGAMI AKIMASA, HAYASHI FUMIAKI,: "G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 49, no. 11, 21 June 2021 (2021-06-21), GB , pages 6069 - 6081, XP093097367, ISSN: 0305-1048, DOI: 10.1093/nar/gkab388
XIAOHONG CHENG, XIANGJUN LIU, TAO BING, ZEHUI CAO, DIHUA SHANGGUAN: "General Peroxidase Activity of G-Quadruplex−Hemin Complexes and Its Application in Ligand Screening", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, vol. 48, no. 33, 25 August 2009 (2009-08-25), pages 7817 - 7823, XP055140326, ISSN: 00062960, DOI: 10.1021/bi9006786
DE‐MING KONG; LI‐LI CAI; JUN‐HONG GUO; JING WU; HAN‐XI SHEN: "Characterization of the G‐quadruplex structure of a catalytic DNA with peroxidase activity", BIOPOLYMERS, JOHN WILEY, HOBOKEN, USA, vol. 91, no. 5, 9 January 2009 (2009-01-09), Hoboken, USA, pages 331 - 339, XP071112736, ISSN: 0006-3525, DOI: 10.1002/bip.21135
YOSHINO TOMOKO, MAEDA YOSHIAKI, MATSUNAGA TADASHI: "Bioengineering of Bacterial Magnetic Particles and their Applications in Biotechnology", RECENT PATENTS ON BIOTECHNOLOGY, BENTHAM SCIENCE PUBLISHERS, NL, vol. 4, no. 3, 1 November 2010 (2010-11-01), NL , pages 214 - 225, XP093097370, ISSN: 1872-2083, DOI: 10.2174/187220810793611455
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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