REAL-TIME DETECTION OF NUCLEIC ACID REACTIONS

WIPO Patent Application WO2004027384

A3

A process is provided for using oligonucleotide to which a detectable moiety is attached post-synthesis. A metal-containing fluorescent compound affords real-time detection of nucleic acid elongation, amplification, or hybridization. The process is especially advantageous since a detectable moiety is readily attached to an existing oligonucleotide at an internal nucleotide, rather than being limited to attachment at a 3' or 5' terminus.

JP5289064	Devices and methods for imaging and modifying biological samples
JP5139885	Fluorescence analyzer and analysis method

JOSEPH RICHARD ABRAHAM (US)
DIMEO JAMES JOSEPH (US)

PCT/US2003/029418

November 11, 2004

September 17, 2003

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PERKINELMER LIFE SCIENCES INC (US)
JOSEPH RICHARD ABRAHAM (US)
DIMEO JAMES JOSEPH (US)

G01N21/64; C12N15/09; C12Q1/68; G01N21/78; G01N; (IPC1-7): C12Q1/68

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US20020006643A1	2002-01-17
US20020064788A1	2002-05-30
US20020142304A1	2002-10-03
US6472160B2	2002-10-29

TABOR ET AL: "A single residue in DNA polymerases of the escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides", PNAS USA, vol. 92, July 1995 (1995-07-01), pages 6339 - 6343, XP002009883
KORCH ET AL: "Manganese citrate improves base-calling accuracy in DNA sequencing reactions using rhodamine-based fluorescent dye-terminators", NUCLEIC ACIDS RESEARCH, vol. 27, no. 5, 1999, pages 1405 - 1407, XP002982126
See also references of EP 1554402A4

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