REAL-TIME MONITORING OF AN ANALYTE BY CHROMATOGRAPHY USING AN ON-LINE ASSAY

Title:

REAL-TIME MONITORING OF AN ANALYTE BY CHROMATOGRAPHY USING AN ON-LINE ASSAY

Document Type and Number:

WIPO Patent Application WO1999034220

Kind Code:

A3

Abstract:

The presence of a contaminating analyte in a protein mixture may obscure detection of a protein of interest. In addition, antibody breakthrough is obscured by flow-through of contaminant proteins when loading cell culture fluid onto affinity columns. An on-line chromatographic assay described herein allows real-time measurement of contaminating analyte during purification or antibody breakthrough during loading and collection of a protein of interest in preparative chromatography. Breakthrough curves are generated allowing the user to end loading of cell culture fluid and/or to end effluent collection for maximization of efficient purification and preventing waste of cell culture fluid.

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Inventors:

BLANK GREGORY S
FAHRNER ROBERT LEE
VANDERLAAN MARTIN

Application Number:

PCT/US1998/026172

Publication Date:

September 02, 1999

Filing Date:

December 09, 1998

Export Citation:

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Assignee:

GENENTECH INC (US)

International Classes:

G01N30/62; G01N30/02; (IPC1-7): C07K1/16; G01N30/82

Domestic Patent References:

WO1993020449A1	1993-10-14
WO1996040398A1	1996-12-19
WO1993007168A2	1993-04-15

Foreign References:

US5234586A

1993-08-10

Other References:

N.B. AFEYAN: "Automated real-time immunoassay of biomolecules", NATURE., vol. 358, 13 August 1992 (1992-08-13), LONDON GB, pages 603 - 604, XP002105367
H. A. CHASE: "Rapid chromatographic monitoring of bioprocesses", BIOSENSORS, vol. 2, 1986, ISSN 0265-928X, pages 269 - 286, XP002105368
LEVISON P R ET AL: "Influence of flow-rate on the chromatographic performance of agarose- and cellulose-based anion-exchange media", JOURNAL OF CHROMATOGRAPHY A, vol. 734, no. 1, 17 May 1996 (1996-05-17), pages 137-143, XP004039088

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