Title:
REASSORTANT INFLUENZA VIRUS PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2018/012498
Kind Code:
A1
Abstract:
Provided is a method for producing a reassortant influenza virus having genome segments from two or more influenza virus strains. This method for producing a reassortant influenza virus containing an antigen protein from a first influenza virus strain comprises the following steps: 1) A step in which the first influenza virus strain is irradiated with ultraviolet rays at a dose at which the virus has reduced or no proliferation capacity and maintains initial infectivity; 2) a step in which a host is infected with the first influenza virus strain and a second influenza virus strain; 3) a step in which the host infected with the first influenza virus strain and the second influenza virus strain is cultured to obtain a culture; 4) a step in which, from the culture obtained at step 3), an influenza virus strain having an antigen protein of the second influenza virus strain is deactivated; and 5) a step in which a reassortant influenza virus is recovered following step 4).
Inventors:
FUJIMOTO TAKAO (JP)
FUJIRA JUNJI (JP)
FUJIRA JUNJI (JP)
Application Number:
PCT/JP2017/025274
Publication Date:
January 18, 2018
Filing Date:
July 11, 2017
Export Citation:
Assignee:
UNIV OSAKA RES FOUND FOR MICROBIAL DISEASES (JP)
International Classes:
C12N7/02; A61K39/00; A61K39/145
Domestic Patent References:
| WO2011145081A1 | 2011-11-24 |
Foreign References:
| JPH10503093A | 1998-03-24 | |||
| JP2016140366A | 2016-08-08 |
Other References:
FONVILLE, JM ET AL.: "Influenza Virus Reassortment Is Enhanced By Semi-infectious Particles but Can Be Suppressed by Defective Interfering Particles", PLOS PATHOG, vol. 11, no. 10, 6 October 2015 (2015-10-06), pages e1005204, XP055561973
RACANIELLO, VR ET AL.: "Isolation of Influenza C Virus Recombinants", JOURNAL OF VIROLOGY, vol. 32, no. 3, December 1979 (1979-12-01), pages 1006 - 1014, XP055561982
NEUMANN ET AL., PNAS, vol. 102, 1999, pages 16825 - 16829
PLOS PATHOG, vol. 11, no. 10, October 2015 (2015-10-01), pages e1005204
J VIROL., vol. 20, no. 1, October 1976 (1976-10-01), pages 248 - 54
"Special Experimental Virology", 28 February 1982, MARUZEN PUBLISHING CO., LTD., pages: 321 - 325
"Influenza Diagnosis Manual", September 2014
See also references of EP 3486316A4
RACANIELLO, VR ET AL.: "Isolation of Influenza C Virus Recombinants", JOURNAL OF VIROLOGY, vol. 32, no. 3, December 1979 (1979-12-01), pages 1006 - 1014, XP055561982
NEUMANN ET AL., PNAS, vol. 102, 1999, pages 16825 - 16829
PLOS PATHOG, vol. 11, no. 10, October 2015 (2015-10-01), pages e1005204
J VIROL., vol. 20, no. 1, October 1976 (1976-10-01), pages 248 - 54
"Special Experimental Virology", 28 February 1982, MARUZEN PUBLISHING CO., LTD., pages: 321 - 325
"Influenza Diagnosis Manual", September 2014
See also references of EP 3486316A4
Attorney, Agent or Firm:
SHOJI, Takashi et al. (JP)
Download PDF: