Title:
RECOMBINANT ADENOVIRUS WITH INCREASED SAFETY AND ANTICANCER ACTIVITIES, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/081229
Kind Code:
A1
Abstract:
The present invention relates to a recombinant adenovirus with increased in-vivo safety, tissue specificity, and anticancer activities, and a use thereof. Specifically, the recombinant adenovirus comprising: a promoter of the liver tissue-specific phosphoenolpyruvate carboxykinase (PEPCK) gene; a trans-splicing ribozyme which is operably linked to the promoter and acts on a cancer-specific gene; a therapeutic gene or a reporter gene which is linked to the 3' exon of the ribozyme; and a serotype 35 fiber knob and a serotype 5 shaft, in which the orf4 gene is deleted from adenovirus E1, E3 and E4 orf1, shows remarkable in-vivo safety, high specificity for a target tissue, and remarkable anticancer effects, and thus can be useful for an anticancer drug or a cancer diagnostic agent as a gene delivery vector.
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Inventors:
LEE SANG-JIN (KR)
KIM YUN-HEE (KR)
KIM IN-HOO (KR)
LEE SEONG-WOOK (KR)
JEONG JIN-SOOK (KR)
HAN SANG YOUNG (KR)
KIM YUN-HEE (KR)
KIM IN-HOO (KR)
LEE SEONG-WOOK (KR)
JEONG JIN-SOOK (KR)
HAN SANG YOUNG (KR)
Application Number:
PCT/KR2013/010639
Publication Date:
May 30, 2014
Filing Date:
November 21, 2013
Export Citation:
Assignee:
NAT CANCER CT (KR)
International Classes:
C12N7/01; A61K35/76; C12Q1/02
Domestic Patent References:
| WO1997016459A1 | 1997-05-09 | |||
| WO1996030385A1 | 1996-10-03 | |||
| WO1994002167A1 | 1994-02-03 |
Foreign References:
| KR20100024055A | 2010-03-05 | |||
| KR20110103119A | 2011-09-20 | |||
| KR20070085254A | 2007-08-27 | |||
| US5783666A | 1998-07-21 |
Other References:
CHO, KYUNG-SOOK.: "In Vivo Liver Toxicity of Cancer-Targeting GeneTherapy Using Trans-splicing Ribozyme", THESIS (MA), 2008, XP008179541
SONG, MIN-SUN ET AL.: "Cancer-selective induction of cytotoxicity by tissue-specific expression of targeted trans-splicing ribozyme.", FEBS LETTERS, vol. 580, 2006, pages 5033 - 5043, XP028061533
LEE, EUN JIG.: "Targeted gene expression using adenoviral vectors.", BIOCHEMISTRY AND MOLECULAR BIOLOGY NEWS., September 2005 (2005-09-01), pages 213 - 221, XP008179352
BEEN, M.; CECH, T.: "One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity", CELL, vol. 47, 1986, pages 207 - 216
SULLENGER, B.A.; CECH, T.R.: "Ribozyme-mediated repair of defective mRNA by targeted, trans-splicing", NATURE, vol. 371, 1994, pages 619 - 622
JONES, J.T.; LEE, S.W.; SULLENGER, B.A.: "Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells", NAT MED., vol. 2, 1996, pages 643 - 648
NAGAO K; TOMIMATSU M; ENDO H ET AL.: "Telomerase reverse trasncriptase mRNA expression and telomerase activity in hepatocellular carcinoma", J GASTRENTEROL, vol. 34, 1999, pages 83 - 87
BRYAN, T.M.; CECH, T.R.: "Telomerase and the maintenance of chromosome ends", CURR. OPIN. CELL BIOL., vol. 11, 1999, pages 318 - 324
BRYAN, T.M.; ENGLEZOU, A.; GUPTA, J.; BACCHETTI, S.; REDDEL, R.R: "Telomere elongation in immortal human cells without detectable telomerase activity", EMBO J., vol. 14, 1995, pages 4240 - 4248
ARTANDI, S.E.; DEPINHO, R.A.: "Mice without telomerase: what can they teach us about human cancer", NAT. MED., vol. 6, 2000, pages 852 - 855
SHAYAKHMETOV DM, J VIROL., vol. 74, no. 22, November 2000 (2000-11-01), pages 10274 - 86
YEH P; DEDIEU JF, J VIROL., vol. 70, no. 1, January 1996 (1996-01-01), pages 559 - 65
LEE ET AL., NATURE, vol. 329, 1987, pages 642
CHENG ET AL., PROC. NAT. ACAD. SCI., vol. 95, 1998, pages 3042 - 3047
DE WET J. ET AL., MOL. CELL BIOL., vol. 7, 1987, pages 725 - 737
LORENZ W.W. ET AL., PNAS, vol. 88, 1991, pages 4438 - 42
GORMAN C. ET AL., MOL. CELL BIOL., vol. 2, 1982, pages 1044 - 1051
HALL C.V. ET AL., J. MOL. APPL. GENET., vol. 2, 1983, pages 101 - 109
SELDEN R. ET AL., MOL. CELL BIOL., vol. 6, 1986, pages 3173 - 3179
CHALFIE M. ET AL., SCIENCE, vol. 263, 1994, pages 802 - 805
BERGER, J. ET AL., GENE, vol. 66, 1988, pages 1 - 10
KWON ET AL., MOL. THER., vol. 12, 2005, pages 824 - 834
KWON, B.S. ET AL., FEBS LETT, vol. 580, 2006, pages 5033 - 5043
SONG MS, CANCER GENE THER, vol. 16, 2009, pages 113 - 125
See also references of EP 2924115A4
SONG, MIN-SUN ET AL.: "Cancer-selective induction of cytotoxicity by tissue-specific expression of targeted trans-splicing ribozyme.", FEBS LETTERS, vol. 580, 2006, pages 5033 - 5043, XP028061533
LEE, EUN JIG.: "Targeted gene expression using adenoviral vectors.", BIOCHEMISTRY AND MOLECULAR BIOLOGY NEWS., September 2005 (2005-09-01), pages 213 - 221, XP008179352
BEEN, M.; CECH, T.: "One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity", CELL, vol. 47, 1986, pages 207 - 216
SULLENGER, B.A.; CECH, T.R.: "Ribozyme-mediated repair of defective mRNA by targeted, trans-splicing", NATURE, vol. 371, 1994, pages 619 - 622
JONES, J.T.; LEE, S.W.; SULLENGER, B.A.: "Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells", NAT MED., vol. 2, 1996, pages 643 - 648
NAGAO K; TOMIMATSU M; ENDO H ET AL.: "Telomerase reverse trasncriptase mRNA expression and telomerase activity in hepatocellular carcinoma", J GASTRENTEROL, vol. 34, 1999, pages 83 - 87
BRYAN, T.M.; CECH, T.R.: "Telomerase and the maintenance of chromosome ends", CURR. OPIN. CELL BIOL., vol. 11, 1999, pages 318 - 324
BRYAN, T.M.; ENGLEZOU, A.; GUPTA, J.; BACCHETTI, S.; REDDEL, R.R: "Telomere elongation in immortal human cells without detectable telomerase activity", EMBO J., vol. 14, 1995, pages 4240 - 4248
ARTANDI, S.E.; DEPINHO, R.A.: "Mice without telomerase: what can they teach us about human cancer", NAT. MED., vol. 6, 2000, pages 852 - 855
SHAYAKHMETOV DM, J VIROL., vol. 74, no. 22, November 2000 (2000-11-01), pages 10274 - 86
YEH P; DEDIEU JF, J VIROL., vol. 70, no. 1, January 1996 (1996-01-01), pages 559 - 65
LEE ET AL., NATURE, vol. 329, 1987, pages 642
CHENG ET AL., PROC. NAT. ACAD. SCI., vol. 95, 1998, pages 3042 - 3047
DE WET J. ET AL., MOL. CELL BIOL., vol. 7, 1987, pages 725 - 737
LORENZ W.W. ET AL., PNAS, vol. 88, 1991, pages 4438 - 42
GORMAN C. ET AL., MOL. CELL BIOL., vol. 2, 1982, pages 1044 - 1051
HALL C.V. ET AL., J. MOL. APPL. GENET., vol. 2, 1983, pages 101 - 109
SELDEN R. ET AL., MOL. CELL BIOL., vol. 6, 1986, pages 3173 - 3179
CHALFIE M. ET AL., SCIENCE, vol. 263, 1994, pages 802 - 805
BERGER, J. ET AL., GENE, vol. 66, 1988, pages 1 - 10
KWON ET AL., MOL. THER., vol. 12, 2005, pages 824 - 834
KWON, B.S. ET AL., FEBS LETT, vol. 580, 2006, pages 5033 - 5043
SONG MS, CANCER GENE THER, vol. 16, 2009, pages 113 - 125
See also references of EP 2924115A4
Attorney, Agent or Firm:
HWANG MOK PARK IP GROUP (KR)
특허법인 충정 (KR)
특허법인 충정 (KR)
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