Title:
RECOMBINANT ANTIBODIES HAVING DUAL SPECIFICITY FOR GANGLIOSIDES AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2013/127373
Kind Code:
A3
Abstract:
The present invention relates to novel monoclonal antibodies and fragments of said antibodies, which have dual specificity and a high affinity for the gangliosides N-acetyl GM3 and N-glicolyl GM3, and which do not recognize other gangliosides. According to another aspect, the present invention relates to the use of said antibodies and the fragments thereof in tumor therapy, in which said tumors have a significant expression of any of the two antigens recognized by said antibodies, or a mixed expression of both antigens. The invention also relates to a use of said antibodies in the diagnosis of tumors that express at least one of the antigens.
Inventors:
MORENO FRIAS ERNESTO (CU)
ROJAS DORANTES GERTRUDIS (CU)
CASADESUS PAZOS ANA VICTORIA (CU)
ROJAS DORANTES GERTRUDIS (CU)
CASADESUS PAZOS ANA VICTORIA (CU)
Application Number:
PCT/CU2013/000001
Publication Date:
October 31, 2013
Filing Date:
February 21, 2013
Export Citation:
Assignee:
CENTRO INMUNOLOGIA MOLECULAR (CU)
International Classes:
C07K16/30
Foreign References:
| EP1623997A1 | 2006-02-08 |
Other References:
WATARAI S ET AL: "PRODUCTION OF MONOCLONAL ANTIBODIES DIRECTED TO HANGANUTZIU-DEICHER ACTIVE GANGLIOSIDES, N-GLYCOLYLNEURAMINIC ACID-CONTAINING GANGLIOSIDES", JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOCIETY / OUP, TOKYO; JP, vol. 117, no. 5, 1 January 1995 (1995-01-01), pages 1062 - 1069, XP008049192, ISSN: 0021-924X
HOON D S ET AL: "MOLECULAR CLONING OF A HUMAN MONOCLONAL ANTIBODY REACTIVE TO GANGLIOSIDE GM3 ANTIGEN ON HUAMN CANCERS", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 53, no. 21, 1 November 1993 (1993-11-01), pages 5244 - 5250, XP002017118, ISSN: 0008-5472
ROJAS GERTRUDIS ET AL: "Engineering the Binding Site of an Antibody against N-Glycolyl GM3: From Functional Mapping to Novel Anti-ganglioside Specificities", ACS CHEMICAL BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 8, no. 2, 1 February 2013 (2013-02-01), pages 376 - 386, XP009171240, ISSN: 1554-8929, DOI: 10.1021/CB30037541
U. KRENGEL: "Structure and Molecular Interactions of a Unique Antitumor Antibody Specific for N-Glycolyl GM3", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, no. 7, 15 December 2003 (2003-12-15), pages 5597 - 5603, XP055071570, ISSN: 0021-9258, DOI: 10.1074/jbc.M311693200
ROJAS G ET AL: "Light-chain shuffling results in successful phage display selection of functional prokaryotic-expressed antibody fragments to N-glycolyl GM3 ganglioside", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 293, no. 1-2, 1 October 2004 (2004-10-01), pages 71 - 83, XP004634276, ISSN: 0022-1759, DOI: 10.1016/J.JIM.2004.07.002
HOON D S ET AL: "MOLECULAR CLONING OF A HUMAN MONOCLONAL ANTIBODY REACTIVE TO GANGLIOSIDE GM3 ANTIGEN ON HUAMN CANCERS", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 53, no. 21, 1 November 1993 (1993-11-01), pages 5244 - 5250, XP002017118, ISSN: 0008-5472
ROJAS GERTRUDIS ET AL: "Engineering the Binding Site of an Antibody against N-Glycolyl GM3: From Functional Mapping to Novel Anti-ganglioside Specificities", ACS CHEMICAL BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 8, no. 2, 1 February 2013 (2013-02-01), pages 376 - 386, XP009171240, ISSN: 1554-8929, DOI: 10.1021/CB30037541
U. KRENGEL: "Structure and Molecular Interactions of a Unique Antitumor Antibody Specific for N-Glycolyl GM3", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 279, no. 7, 15 December 2003 (2003-12-15), pages 5597 - 5603, XP055071570, ISSN: 0021-9258, DOI: 10.1074/jbc.M311693200
ROJAS G ET AL: "Light-chain shuffling results in successful phage display selection of functional prokaryotic-expressed antibody fragments to N-glycolyl GM3 ganglioside", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 293, no. 1-2, 1 October 2004 (2004-10-01), pages 71 - 83, XP004634276, ISSN: 0022-1759, DOI: 10.1016/J.JIM.2004.07.002
Attorney, Agent or Firm:
LÓPEZ MATILLA, Lien (Atabey. Playa, La Habana, CU)
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