Title:
RECOMBINANT BACILLUS SUBTILIS CAPABLE OF INCREASING YIELD OF MENAQUINONE-7 AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/244527
Kind Code:
A1
Abstract:
Provided is a recombinant Bacillus subtilis capable of increasing the yield of menaquinone-7, characterized by using Bacillus subtilis 168 as a starting strain, and replacing natural promoters of menaquinone isochorismate synthase gene menF and dihydroxynaphthoic acid synthase gene menB on a chromosome with a P43 promoter; replacing natural promoters of O-succinylbenzoic acid-CoA ligase gene menE and transketolase gene tkt on the chromosome with a Phbs promoter; and expressing an exogenous isochorismate synthase entC gene and an exogenous phosphoenolpyruvate synthase ppsA gene on the chromosome with the P43 promoter, and knocking out glucose phosphotransferase gene ptsG on the chromosome.
Inventors:
XIA HONGZHI (CN)
LIU LONG (CN)
GU QINQING (CN)
LI JIANGBO (CN)
CUI SHIXIU (CN)
LV XUEQIN (CN)
LI JIANGHUA (CN)
DU GUOCHENG (CN)
CHEN JIAN (CN)
LIU LONG (CN)
GU QINQING (CN)
LI JIANGBO (CN)
CUI SHIXIU (CN)
LV XUEQIN (CN)
LI JIANGHUA (CN)
DU GUOCHENG (CN)
CHEN JIAN (CN)
Application Number:
PCT/CN2020/094048
Publication Date:
December 10, 2020
Filing Date:
June 03, 2020
Export Citation:
Assignee:
NANTONG LICHENG BIOLOGICAL ENG CO LTD (CN)
International Classes:
C12N1/21; C12P7/66; C12R1/125
Foreign References:
| CN110229772A | 2019-09-13 | |||
| CN108715825A | 2018-10-30 | |||
| CN108715824A | 2018-10-30 | |||
| CN108676766A | 2018-10-19 |
Other References:
WANG, HUA ET AL.: "Regulation of Shikimic Acid Biosynthesis Pathway", BIOTECHNOLOGY BULLETIN, no. 3, 15 May 2009 (2009-05-15), ISSN: 1002-5464, DOI: 20200827232315A
WANG, HUA ET AL.: "Rational design and construction of an overproducing shikimic acid Escherichia coli by metabolic engineering", BIOTECHNOLOGY BULLETIN, vol. 29, no. 1,, 25 January 2013 (2013-01-25), ISSN: 1000-3061, DOI: 20200827232510A
YANG, SEN: "Construction of Gene Regulation and Expression Systems in Bacillus Subtilis and Its Application in Fermentation Engineering", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE (ELECTRONIC JOURNAL) BASIC SCIENCES), no. 4, 15 April 2018 (2018-04-15), ISSN: 1674-022X, DOI: 20200827233055A
XU, JIANZHONG ET AL.: "Non-official translation: Effects of Major Enzymes in the Menaquinone-7 (MK-7) Biosynthetic Pathway", BIOTECHNOLOGY BULLETIN, vol. 32, no. 11,, 11 December 2016 (2016-12-11), ISSN: 1002-5464, DOI: 20200828005428A
WANG, HUA ET AL.: "Rational design and construction of an overproducing shikimic acid Escherichia coli by metabolic engineering", BIOTECHNOLOGY BULLETIN, vol. 29, no. 1,, 25 January 2013 (2013-01-25), ISSN: 1000-3061, DOI: 20200827232510A
YANG, SEN: "Construction of Gene Regulation and Expression Systems in Bacillus Subtilis and Its Application in Fermentation Engineering", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE (ELECTRONIC JOURNAL) BASIC SCIENCES), no. 4, 15 April 2018 (2018-04-15), ISSN: 1674-022X, DOI: 20200827233055A
XU, JIANZHONG ET AL.: "Non-official translation: Effects of Major Enzymes in the Menaquinone-7 (MK-7) Biosynthetic Pathway", BIOTECHNOLOGY BULLETIN, vol. 32, no. 11,, 11 December 2016 (2016-12-11), ISSN: 1002-5464, DOI: 20200828005428A
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM (ORDINARY PARTNERSHIP) (CN)
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