Title:
RECOMBINANT BACTERIA COMPRISING NOVEL SUCROSE TRANSPORTERS
Document Type and Number:
WIPO Patent Application WO/2013/134174
Kind Code:
A3
Abstract:
Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
More Like This:
Inventors:
RUEBLING-JASS KRISTIN (US)
TOMB JEAN-FRANCOIS (US)
VAN DYK TINA K (US)
YOU ZHENG (US)
TOMB JEAN-FRANCOIS (US)
VAN DYK TINA K (US)
YOU ZHENG (US)
Application Number:
PCT/US2013/028970
Publication Date:
December 27, 2013
Filing Date:
March 05, 2013
Export Citation:
Assignee:
DU PONT (US)
International Classes:
C07K14/195; C07K14/24; C07K14/245; C07K14/33; C07K14/335; C12N9/10; C12N9/24; C12P7/18; C12P7/20; C12P7/42
Domestic Patent References:
| WO2013025286A1 | 2013-02-21 |
Foreign References:
| US20110136190A1 | 2011-06-09 | |||
| US20010049126A1 | 2001-12-06 |
Other References:
DATABASE UniProt [online] 26 May 2009 (2009-05-26), "SubName: Full=Sucrose transporter;", XP002705616, retrieved from EBI accession no. UNIPROT:C1M5D2 Database accession no. C1M5D2
DATABASE UniProt [online] 22 February 2012 (2012-02-22), "SubName: Full=Oligosaccharide:H+ symporter;", XP002705617, retrieved from EBI accession no. UNIPROT:G9SK52 Database accession no. G9SK52
JAHREIS KNUT ET AL: "Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132.", JOURNAL OF BACTERIOLOGY OCT 2002, vol. 184, no. 19, October 2002 (2002-10-01), pages 5307 - 5316, XP002705618, ISSN: 0021-9193
MONICA M OLSON ET AL: "Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 74, no. 5, 11 January 2007 (2007-01-11), pages 1031 - 1040, XP019489541, ISSN: 1432-0614, DOI: 10.1007/S00253-006-0746-2
TSUNEKAWA H ET AL: "ACQUISITION OF A SUCROSE UTILIZATION SYSTEM IN ESCHERICHIA COLI K-12 DERIVATIVES AND ITS APPLICATION TO INDUSTRY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 58, no. 6, 1 June 1992 (1992-06-01), pages 2081 - 2088, XP001024474, ISSN: 0099-2240
DATABASE UniProt [online] 10 May 2005 (2005-05-10), "SubName: Full=Sugar transport protein;", XP002713332, retrieved from EBI accession no. UNIPROT:Q564C6 Database accession no. Q564C6
JANER C ET AL: "Hydrolysis of Oligofructoses by the Recombinant beta-Fructofuranosidase from Bifidobacterium lactis", SYSTEMATIC AND APPLIED MICROBIOLOGY, URBAN & FISCHER, AMSTERDAM, NL, vol. 27, no. 3, 1 January 2004 (2004-01-01), pages 279 - 285, XP004957499, ISSN: 0723-2020, DOI: 10.1078/0723-2020-00274
DATABASE UniProt [online] 9 February 2010 (2010-02-09), "SubName: Full=Sucrose transport protein;", XP002713333, retrieved from EBI accession no. UNIPROT:D1NSR6 Database accession no. D1NSR6
DATABASE UniProt [online] 1 March 2003 (2003-03-01), "SubName: Full=Sucrose transport protein;", XP002713334, retrieved from EBI accession no. UNIPROT:Q8G7X9 Database accession no. Q8G7X9
SCHELL M A ET AL: "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES - PNAS, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14422 - 14427, XP002313989, ISSN: 0027-8424, DOI: 10.1073/PNAS.212527599
DATABASE UniProt [online] 23 January 2007 (2007-01-23), "SubName: Full=Sucrose transport protein;", XP002713335, retrieved from EBI accession no. UNIPROT:A1A2J7 Database accession no. A1A2J7
DATABASE UniProt [online] 22 February 2012 (2012-02-22), "SubName: Full=Oligosaccharide:H+ symporter;", XP002705617, retrieved from EBI accession no. UNIPROT:G9SK52 Database accession no. G9SK52
JAHREIS KNUT ET AL: "Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132.", JOURNAL OF BACTERIOLOGY OCT 2002, vol. 184, no. 19, October 2002 (2002-10-01), pages 5307 - 5316, XP002705618, ISSN: 0021-9193
MONICA M OLSON ET AL: "Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 74, no. 5, 11 January 2007 (2007-01-11), pages 1031 - 1040, XP019489541, ISSN: 1432-0614, DOI: 10.1007/S00253-006-0746-2
TSUNEKAWA H ET AL: "ACQUISITION OF A SUCROSE UTILIZATION SYSTEM IN ESCHERICHIA COLI K-12 DERIVATIVES AND ITS APPLICATION TO INDUSTRY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 58, no. 6, 1 June 1992 (1992-06-01), pages 2081 - 2088, XP001024474, ISSN: 0099-2240
DATABASE UniProt [online] 10 May 2005 (2005-05-10), "SubName: Full=Sugar transport protein;", XP002713332, retrieved from EBI accession no. UNIPROT:Q564C6 Database accession no. Q564C6
JANER C ET AL: "Hydrolysis of Oligofructoses by the Recombinant beta-Fructofuranosidase from Bifidobacterium lactis", SYSTEMATIC AND APPLIED MICROBIOLOGY, URBAN & FISCHER, AMSTERDAM, NL, vol. 27, no. 3, 1 January 2004 (2004-01-01), pages 279 - 285, XP004957499, ISSN: 0723-2020, DOI: 10.1078/0723-2020-00274
DATABASE UniProt [online] 9 February 2010 (2010-02-09), "SubName: Full=Sucrose transport protein;", XP002713333, retrieved from EBI accession no. UNIPROT:D1NSR6 Database accession no. D1NSR6
DATABASE UniProt [online] 1 March 2003 (2003-03-01), "SubName: Full=Sucrose transport protein;", XP002713334, retrieved from EBI accession no. UNIPROT:Q8G7X9 Database accession no. Q8G7X9
SCHELL M A ET AL: "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES - PNAS, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14422 - 14427, XP002313989, ISSN: 0027-8424, DOI: 10.1073/PNAS.212527599
DATABASE UniProt [online] 23 January 2007 (2007-01-23), "SubName: Full=Sucrose transport protein;", XP002713335, retrieved from EBI accession no. UNIPROT:A1A2J7 Database accession no. A1A2J7
Attorney, Agent or Firm:
CHRISTENBURY, Lynne, M. (Legal Patent Records Center4417 Lancaster Pik, Wilmington Delaware, US)
Download PDF: