Title:
RECOMBINANT CELL AND METHOD FOR PRODUCING ISOPRENE
Document Type and Number:
WIPO Patent Application WO/2014/104202
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a series of techniques for producing isoprene from methanol, etc.
Provided is a recombinant cell wherein a gene encoding isoprene synthase is transferred into a host cell that is a methylotroph, wherein said gene can be expressed in the host cell and is capable of producing isoprene from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde and formamide. The recombinant cell preferably has, as a formaldehyde fixation pathway, at least one C1 carbon assimilation pathway selected from the group consisting of the serine pathway, ribulose monophosphate pathway and xylose monophosphate pathway. Also provided is a method for producing isoprene by using the aforesaid recombinant cell.
Inventors:
FURUTANI MASAHIRO (JP)
UENISHI AKIHIRO (JP)
IWASA KOICHIRO (JP)
UENISHI AKIHIRO (JP)
IWASA KOICHIRO (JP)
Application Number:
PCT/JP2013/084915
Publication Date:
July 03, 2014
Filing Date:
December 26, 2013
Export Citation:
Assignee:
SEKISUI CHEMICAL CO LTD (JP)
International Classes:
C12N1/19; C12N1/21; C12N15/09; C12P5/02
Domestic Patent References:
| WO2009076676A2 | 2009-06-18 | |||
| WO2009132220A2 | 2009-10-29 |
Foreign References:
| JP2011505841A | 2011-03-03 | |||
| JP2008035831A | 2008-02-21 | |||
| US5849970A | 1998-12-15 | |||
| JP2004229662A | 2004-08-19 |
Other References:
YANG, J. ET AL.: "Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli", BIORESOUR. TECHNOL., vol. 104, January 2012 (2012-01-01), pages 642 - 647, XP055251917
ZHAO, Y. ET AL.: "Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway.", APPL. MICROBIOL. BIOTECHNOL., vol. 90, no. 6, 2011, pages 1915 - 1922, XP019908970
YANG J. ET AL., PLOSONE, vol. 7, no. 4, 2012, pages E33509
PARK H. ET AL., MICROBIOLOGY, vol. 156, 2010, pages 463 - 471
ANTHONY C.: "The Biochemistry of Methylotroph", 1982, ACADEMIC PRESS INC.
BRAUTASET T. ET AL., J. BACTERIOLOGY, vol. 186, no. 5, 2004, pages 1229 - 1238
SIVY TL. ET AL., BIOCHEM. BIOPHYS. RES. COMMU., vol. 294, no. 1, 2002, pages 71 - 5
TAKAGI M. ET AL., J. BACTERIOL., vol. 182, no. 15, 2000, pages 4153 - 7
HAMANO Y. ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 65, no. 7, 2001, pages 1627 - 35
SMEDS A ET AL., DNA SEQ., vol. 12, no. 3, 2001, pages 187 - 190
LOMBARD J. ET AL., MOL. BIOL. EVOL., vol. 28, no. 1, 2010, pages 87 - 99
CHISTOSERDOVA L. ET AL., MICROBIOLOGY, vol. 146, 2000, pages 233 - 238
CHISTOSERDOV AY. ET AL., J. BACTERIOL, vol. 176, 1994, pages 4052 - 4065
CHISTOSERDOV AY. ET AL., PLASMID, vol. 16, 1986, pages 161 - 167
LANE M. ET AL., ARCH. MICROBIOL., vol. 144, no. 1, 1986, pages 29 - 34
ANTOINE R. ET AL., MOLECULAR MICROBIOLOGY, vol. 6, 1992, pages 1785 - 1799
MARX CJ. ET AL., MICROBIOLOGY, vol. 147, 2001, pages 2065 - 2075
NQUYEN HD. ET AL., PLASMID, vol. 54, no. 3, 2005, pages 241 - 248
ZHAO, Y. ET AL.: "Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway.", APPL. MICROBIOL. BIOTECHNOL., vol. 90, no. 6, 2011, pages 1915 - 1922, XP019908970
YANG J. ET AL., PLOSONE, vol. 7, no. 4, 2012, pages E33509
PARK H. ET AL., MICROBIOLOGY, vol. 156, 2010, pages 463 - 471
ANTHONY C.: "The Biochemistry of Methylotroph", 1982, ACADEMIC PRESS INC.
BRAUTASET T. ET AL., J. BACTERIOLOGY, vol. 186, no. 5, 2004, pages 1229 - 1238
SIVY TL. ET AL., BIOCHEM. BIOPHYS. RES. COMMU., vol. 294, no. 1, 2002, pages 71 - 5
TAKAGI M. ET AL., J. BACTERIOL., vol. 182, no. 15, 2000, pages 4153 - 7
HAMANO Y. ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 65, no. 7, 2001, pages 1627 - 35
SMEDS A ET AL., DNA SEQ., vol. 12, no. 3, 2001, pages 187 - 190
LOMBARD J. ET AL., MOL. BIOL. EVOL., vol. 28, no. 1, 2010, pages 87 - 99
CHISTOSERDOVA L. ET AL., MICROBIOLOGY, vol. 146, 2000, pages 233 - 238
CHISTOSERDOV AY. ET AL., J. BACTERIOL, vol. 176, 1994, pages 4052 - 4065
CHISTOSERDOV AY. ET AL., PLASMID, vol. 16, 1986, pages 161 - 167
LANE M. ET AL., ARCH. MICROBIOL., vol. 144, no. 1, 1986, pages 29 - 34
ANTOINE R. ET AL., MOLECULAR MICROBIOLOGY, vol. 6, 1992, pages 1785 - 1799
MARX CJ. ET AL., MICROBIOLOGY, vol. 147, 2001, pages 2065 - 2075
NQUYEN HD. ET AL., PLASMID, vol. 54, no. 3, 2005, pages 241 - 248
Attorney, Agent or Firm:
FUJITA Takashi et al. (JP)
Takashi Fujita (JP)
Takashi Fujita (JP)
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