Title:
RECOMBINANT COAGULATION FACTOR VIII AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/228087
Kind Code:
A1
Abstract:
Provided are recombinant coagulation factor VIII and application use thereof. The recombinant coagulation factor VIII includes more than 80% of an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. Through gene modification of the B domain, the recombinant coagulation factor VIII is rich in glycosylation sites, has a good coagulation function, is easily secreted outside a cell and easily interacts with a cofactor such as thrombin (Ella). In addition, the recombinant coagulation factor VIII has a weak ability to induce an antibody response and can effectively ensure a treatment effect, reduce a risk of immune rejection and lower a treatment cost.
Inventors:
CHANG LUNG-JI (CN)
GONH JIE (CN)
ZHANG RUI (CN)
GONH JIE (CN)
ZHANG RUI (CN)
Application Number:
PCT/CN2022/085853
Publication Date:
November 03, 2022
Filing Date:
April 08, 2022
Export Citation:
Assignee:
BEIJING MEIKANG GENO IMMUNE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K14/755; A61K38/37; A61P7/04; C12N5/10; C12N7/01; C12N15/12; C12N15/867
Domestic Patent References:
| WO2019228527A1 | 2019-12-05 |
Foreign References:
| CN113248594A | 2021-08-13 | |||
| CN105017410A | 2015-11-04 |
Other References:
GONG JIE, CHUNG TSAI-HUA, ZHENG JIE, ZHENG HUYONG, CHANG LUNG-JI: "Transduction of modified factor VIII gene improves lentiviral gene therapy efficacy for hemophilia A", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 297, no. 6, 1 December 2021 (2021-12-01), US , pages 101397 - 13, XP055980787, ISSN: 0021-9258, DOI: 10.1016/j.jbc.2021.101397
DATABASE Protein - GenPept NCBI; 26 November 2019 (2019-11-26), ANONYMOUS : "BDD-F8, partial [Cloning vector pCDNA4-BDD-F8]", XP055980839, Database accession no. QGN01200.1
DATABASE Protein - Genpept NCBI; 8 November 1994 (1994-11-08), ANONYMOUS : "factor VIII [Homo sapiens]", XP055980841, Database accession no. AAA52484.1
ZHANG JIAN-PING, CHENG XIN-XIN, ZHAO MEI, LI GUO-HUA, XU JING, ZHANG FENG, YIN MENG-DI, MENG FEI-YING, DAI XIN-YUE, FU YA-WEN, YAN: "Curing hemophilia A by NHEJ-mediated ectopic F8 insertion in the mouse", GENOME BIOLOGY, vol. 20, no. 1, 1 December 2019 (2019-12-01), pages 276 - 17, XP055980843, DOI: 10.1186/s13059-019-1907-9
DATABASE Protein - GenPept NCBI; 26 November 2019 (2019-11-26), ANONYMOUS : "BDD-F8, partial [Cloning vector pCDNA4-BDD-F8]", XP055980839, Database accession no. QGN01200.1
DATABASE Protein - Genpept NCBI; 8 November 1994 (1994-11-08), ANONYMOUS : "factor VIII [Homo sapiens]", XP055980841, Database accession no. AAA52484.1
ZHANG JIAN-PING, CHENG XIN-XIN, ZHAO MEI, LI GUO-HUA, XU JING, ZHANG FENG, YIN MENG-DI, MENG FEI-YING, DAI XIN-YUE, FU YA-WEN, YAN: "Curing hemophilia A by NHEJ-mediated ectopic F8 insertion in the mouse", GENOME BIOLOGY, vol. 20, no. 1, 1 December 2019 (2019-12-01), pages 276 - 17, XP055980843, DOI: 10.1186/s13059-019-1907-9
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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