Title:
RECOMBINANT DNA POLYMERASE
Document Type and Number:
WIPO Patent Application WO/2018/126470
Kind Code:
A1
Abstract:
A recombinant DNA polymerase is provided, being one of the following A) to C): A) the indicated protein is a protein with DNA polymerase activity obtained by modifying amino acid residues 408, 409 and 485 in a wild type KOD DNA polymerase amino acid sequence and by modifying at least one of amino acid residues 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 in the wild type KOD DNA polymerase amino acid sequence; B) the indicated protein is a derivative protein from the protein in A), has DNA polymerase activity, and is obtained by removing amino acids 1-29 from the C-terminal of the amino acid sequence of the protein in A), retaining the other amino acid residues and; and C) the indicated protein is a derivative protein of the protein in A) or B), has DNA polymerase activity, and is obtained by adding a tag sequence at the terminal of the amino acid sequence of the protein in A) or B).
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Inventors:
WANG LIN (CN)
LIU FEN (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU CHONGJUN (CN)
SNEZANA DRMANAC (CN)
LIU FEN (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU CHONGJUN (CN)
SNEZANA DRMANAC (CN)
Application Number:
PCT/CN2017/070609
Publication Date:
July 12, 2018
Filing Date:
January 09, 2017
Export Citation:
Assignee:
MGI TECH CO LTD (CN)
International Classes:
C12N9/12
Domestic Patent References:
| WO2015200693A1 | 2015-12-30 |
Foreign References:
| CN104854237A | 2015-08-19 |
Other References:
HASHIMOTO, H: "Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KODI", JOURNAL OF MOLECULAR BIOLOGY, vol. 306, no. 3, 23 February 2001 (2001-02-23), pages 469 - 477, XP004469251, ISSN: 0022-2836
NISHIOKA, M. ET AL., J. BIOTECHNOL., vol. 88, 2001
See also references of EP 3613851A4
NISHIOKA, M. ET AL., J. BIOTECHNOL., vol. 88, 2001
See also references of EP 3613851A4
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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