Title:
RECOMBINANT ESCHERICHIA COLI AND METHOD FOR PRODUCING TANSHINOL BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/200873
Kind Code:
A1
Abstract:
Provided is a genetically engineered bacterium. The bacterium co-expresses α-hydroxyl carboxylic acid dehydrogenase, L-amino-acid oxidase, and any one of groups consisting of free exogenous L-glutamate dehydrogenase, exogenous L-lactic dehydrogenase, or a co-expression combination of glucose dehydrogenase and tyrosine phenol lyase, and genes except for those related to a phenolic compound decomposition are knocked out. The genetically engineered bacterium can be used for producing optically pure 3-(3,4-dihydroxyphenyl)-hydroxypropionic acid. Also provided is a method for producing tanshinol by using the genetically engineered bacterium.
Inventors:
CAI YUJIE (CN)
XIONG TIANZHEN (CN)
LIU JINBIN (CN)
DING YANRUI (CN)
BAI YAJUN (CN)
ZHENG XIAOHUI (CN)
XIONG TIANZHEN (CN)
LIU JINBIN (CN)
DING YANRUI (CN)
BAI YAJUN (CN)
ZHENG XIAOHUI (CN)
Application Number:
PCT/CN2018/111884
Publication Date:
October 24, 2019
Filing Date:
October 25, 2018
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N15/53; C12N15/60; C12P7/40; C12P7/42; C12R1/19
Foreign References:
CN107299072A | 2017-10-27 | |||
CN103667371A | 2014-03-26 | |||
CN101565694A | 2009-10-28 | |||
CN107916245A | 2018-04-17 |
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
Download PDF: