Title:
RECOMBINANT ESCHERICHIA COLI FOR PRODUCING L-HOMOSERINE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/240794
Kind Code:
A1
Abstract:
The present invention relates to recombinant Escherichia coli for producing L-homoserine and the use thereof. L-homoserine synthesis related genes in Escherichia coli are modified on a genome level by means of metabolic engineering; and a genetically engineered bacterium which is clear in genetic background, does not carry plasmids, does not need to be induced and can stably and efficiently produce L-homoserine is obtained by means of weakening the flux of a degradation pathway, i.e., an L-threonine synthesis pathway, enhancing the metabolic flow of the L-homoserine synthesis pathway, enhancing the supply of precursors oxaloacetic acid and L-aspartic acid, promoting the extracellular transfer of L-homoserine, and promoting the co-utilization of cofactors. The bacterium has application potential in large-scale production.
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Inventors:
RAO ZHIMING (CN)
CAI MENGMENG (CN)
XU MEIJUAN (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
CAI MENGMENG (CN)
XU MEIJUAN (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
Application Number:
PCT/CN2022/114482
Publication Date:
December 21, 2023
Filing Date:
August 24, 2022
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12N15/70; C12P13/06; C12R1/19
Domestic Patent References:
| WO2018152918A1 | 2018-08-30 |
Foreign References:
| CN112779204A | 2021-05-11 | |||
| CN109666617A | 2019-04-23 | |||
| CN109055290A | 2018-12-21 | |||
| CN112592875A | 2021-04-02 | |||
| CN108504613A | 2018-09-07 | |||
| CN113122487A | 2021-07-16 | |||
| CN113151127A | 2021-07-23 |
Other References:
HUANG JIAN-FENG, ZHANG BO, SHEN ZHEN-YANG, LIU ZHI-QIANG, ZHENG YU-GUO: "Metabolic engineering of E. coli for the production of O-succinyl-l-homoserine with high yield", 3 BIOTECH, SPRINGEROPEN, DE, vol. 8, no. 7, 1 July 2018 (2018-07-01), DE , XP093118013, ISSN: 2190-572X, DOI: 10.1007/s13205-018-1332-x
LIU PENG, ZHANG BO, YAO ZHEN-HAO, LIU ZHI-QIANG, ZHENG YU-GUO: "Multiplex Design of the Metabolic Network for Production of l -Homoserine in Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 86, no. 20, 1 October 2020 (2020-10-01), US , XP093118014, ISSN: 0099-2240, DOI: 10.1128/AEM.01477-20
LIU PENG, ZHANG BO, YAO ZHEN-HAO, LIU ZHI-QIANG, ZHENG YU-GUO: "Multiplex Design of the Metabolic Network for Production of l -Homoserine in Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 86, no. 20, 1 October 2020 (2020-10-01), US , XP093118014, ISSN: 0099-2240, DOI: 10.1128/AEM.01477-20
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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