Title:
RECOMBINANT KOD POLYMERASE
Document Type and Number:
WIPO Patent Application WO/2022/082482
Kind Code:
A1
Abstract:
Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.
Inventors:
ZHAI LILI (CN)
XIE QINGQING (CN)
CAO RUYIN (CN)
WANG ZI (CN)
ZHENG YUE (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU XUN (CN)
XIE QINGQING (CN)
CAO RUYIN (CN)
WANG ZI (CN)
ZHENG YUE (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU XUN (CN)
Application Number:
PCT/CN2020/122380
Publication Date:
April 28, 2022
Filing Date:
October 21, 2020
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12N9/12; C12Q1/68
Domestic Patent References:
| WO2020047695A1 | 2020-03-12 | |||
| WO2020048329A1 | 2020-03-12 |
Foreign References:
| CN109937252A | 2019-06-25 | |||
| CN108018270A | 2018-05-11 | |||
| CN106536728A | 2017-03-22 | |||
| CN107002051A | 2017-08-01 | |||
| CN2018103764W | 2018-09-03 |
Other References:
O'NEILL ELLIS C., STEVENSON CLARE E. M., PATERSON MICHAEL J., REJZEK MARTIN, CHAUVIN ANNE-LAURE, LAWSON DAVID M., FIELD ROBERT A.: "Crystal structure of a novel two domain GH78 family α-rhamnosidase from K lebsiella oxytoca with rhamnose bound : Crystal Structure of the GH78 Family Rhamnosidase", PROTEINS: STRUCTURE, FUNCTION, AND BIOINFORMATICS, JOHN WILEY & SONS, INC., US, vol. 83, no. 9, 1 September 2015 (2015-09-01), US , pages 1742 - 1749, XP055923674, ISSN: 0887-3585, DOI: 10.1002/prot.24807
NGHI, D.H. ET AL. .: "The Wood Rot Ascomycete Xylaria polymorpha Produces a Novel GH78 Glycoside Hydrolase That Exhibits -L-Rhamnosidase and Feruloyl Esterase Activities and Releases Hydroxycinnamic Acids from Lignocelluloses.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 78, no. 14, 31 July 2012 (2012-07-31), XP055923675
NISHIOKA, M., J. BIOTECHNOL., 2001, pages 88
NGHI, D.H. ET AL. .: "The Wood Rot Ascomycete Xylaria polymorpha Produces a Novel GH78 Glycoside Hydrolase That Exhibits -L-Rhamnosidase and Feruloyl Esterase Activities and Releases Hydroxycinnamic Acids from Lignocelluloses.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 78, no. 14, 31 July 2012 (2012-07-31), XP055923675
NISHIOKA, M., J. BIOTECHNOL., 2001, pages 88
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
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