Title:
RECOMBINANT MICROORGANISM AND L-LEUCINE PRODUCTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/255160
Kind Code:
A1
Abstract:
The present disclosure relates to a recombinant microorganism capable of efficiently generating L-leucine and an L-leucine production method using the recombinant microorganism. The production method includes (p) generating L-leucine by incubating a recombinant microorganism having an L-leucine biosynthetic pathway or a microbial processed product thereof in a predetermined culture medium (X) and (q) recovering a fraction including the L-leucine from the predetermined culture medium (X). The recombinant microorganism includes, in an expressible form, a gene for encoding an amino acid dehydrogenase. The amino acid dehydrogenase has a catalyst effect on at least the L-leucine generation reaction in the L-leucine biosynthetic pathway.
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Inventors:
NAKANE SHUHEI (JP)
Application Number:
PCT/JP2022/021200
Publication Date:
December 08, 2022
Filing Date:
May 24, 2022
Export Citation:
Assignee:
GREEN EARTH INST CO LTD (JP)
ITOCHU CHEMICAL FRONTIER CORP (JP)
ITOCHU CHEMICAL FRONTIER CORP (JP)
International Classes:
C12P13/06; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/53
Domestic Patent References:
| WO2020090016A1 | 2020-05-07 |
Foreign References:
| JPH1023896A | 1998-01-27 | |||
| JP2002233395A | 2002-08-20 |
Other References:
KATAOKA KUNISHIGE, TAKADA HARUMI, TANIZAWA KATSUYUKI, YOSHIMURA TOHRU, ESAKI NOBUYOSHI, OHSHIMA TOSHIHISA, SODA KENJI: "Construction and Characterization of Chimeric Enzyme Consisting of an Amino-Terminal Domain of Phenylalanine Dehydrogenase and a Carboxy-Terminal Domain of Leucine Dehydrogenase", JOURNAL OF BIOCHEMISTRY, OXFORD UNIVERSITY PRESS, GB, vol. 116, no. 4, 1 October 1994 (1994-10-01), GB , pages 931 - 936, XP093009793, ISSN: 0021-924X, DOI: 10.1093/oxfordjournals.jbchem.a124618
Attorney, Agent or Firm:
YAGAMI Ayanobu (JP)
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