Title:
RECOMBINANT MICROORGANISM FOR PRODUCING L-VALINE, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/227500
Kind Code:
A1
Abstract:
A recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an acetohydroxy acid reductoisomerase gene and/or an amino acid dehydrogenase gene into a microorganism, and improving the activity of acetohydroxy acid reductoisomerase and the activity of amino acid dehydrogenase, the yield and conversion rate of L-valine produced by Escherichia coli can be increased, and one-step anaerobic fermentation of L-valine can be achieved.
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Inventors:
ZHANG XUELI (CN)
GUO HENGHUA (CN)
LIU PINGPING (CN)
ZHANG DONGZHU (CN)
TANG JINLEI (CN)
HAN CHENGXIU (CN)
TANG SIQING (CN)
LIU SHUPENG (CN)
MA YANHE (CN)
GUO HENGHUA (CN)
LIU PINGPING (CN)
ZHANG DONGZHU (CN)
TANG JINLEI (CN)
HAN CHENGXIU (CN)
TANG SIQING (CN)
LIU SHUPENG (CN)
MA YANHE (CN)
Application Number:
PCT/CN2020/137780
Publication Date:
November 18, 2021
Filing Date:
December 18, 2020
Export Citation:
Assignee:
ANHUI HUAHENG BIOTECHNOLOGY CO LTD (CN)
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N15/74; C12N1/21; C12P13/08; C12R1/13
Domestic Patent References:
| WO2013009818A2 | 2013-01-17 |
Foreign References:
| CN110607268A | 2019-12-24 | |||
| CN103946370A | 2014-07-23 | |||
| CN104561074A | 2015-04-29 | |||
| US20140017748A1 | 2014-01-16 | |||
| US20090163376A1 | 2009-06-25 | |||
| US6737255B2 | 2004-05-18 |
Other References:
S. HASEGAWA, M. SUDA, K. UEMATSU, Y. NATSUMA, K. HIRAGA, T. JOJIMA, M. INUI, H. YUKAWA: "Engineering of Corynebacterium glutamicum for High-Yield L-Valine Production under Oxygen Deprivation Conditions", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 4, 15 February 2013 (2013-02-15), pages 1250 - 1257, XP055174400, ISSN: 00992240, DOI: 10.1128/AEM.02806-12
S. HASEGAWA, K. UEMATSU, Y. NATSUMA, M. SUDA, K. HIRAGA, T. JOJIMA, M. INUI, H. YUKAWA: "Improvement of the Redox Balance Increases L-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 3, 1 February 2012 (2012-02-01), pages 865 - 875, XP055174358, ISSN: 00992240, DOI: 10.1128/AEM.07056-11
SAVRASOVA EKATERINA A., STOYNOVA NATALIYA V.: "Application of leucine dehydrogenase Bcd from Bacillus subtilis for l-valine synthesis in Escherichia coli under microaerobic conditions", HELIYON, vol. 5, no. 4, 1 April 2019 (2019-04-01), GB , pages e01406, XP055866421, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2019.e01406
ZENG, BANGDING ET AL.: "Effects of Brevibacterium Flavum with Directed Mutagenesis of ilvN and Co-Expression of ilvBNC Cluster on L-Valine Production", CIESC JOURNAL, vol. 67, no. 7, 31 July 2016 (2016-07-31), pages 2956 - 2962, XP055866423
VYAZMENSKY, M ET AL., BIOCHEMISTRY, vol. 35, 1996, pages 10339 - 10346
DE FELICE ET AL., JOURNAL OF BACTERIOLOGY, vol. 120, 1974, pages 1058 - 1067
TAN ET AL., APPL ENVIRON MICROBIOL, vol. 79, 2013, pages 4838 - 4844
DATSENKOWANNER, PROC NATL ACAD SCI USA, vol. 97, 2000, pages 6640 - 6645
LU ET AL., APPL MICROBIOL BIOTECHNOL, vol. 93, 2012, pages 2455 - 2462
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 253, 1978, pages 5719 - 5725
BRINKMANN-CHEN, S.CAHN, JKBARNOLD, FH: "Uncovering rare NADH-preferring ketol-acid reductoisomerases", METAB ENG, vol. 26, 2014, pages 17 - 22
See also references of EP 3929297A4
S. HASEGAWA, K. UEMATSU, Y. NATSUMA, M. SUDA, K. HIRAGA, T. JOJIMA, M. INUI, H. YUKAWA: "Improvement of the Redox Balance Increases L-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, no. 3, 1 February 2012 (2012-02-01), pages 865 - 875, XP055174358, ISSN: 00992240, DOI: 10.1128/AEM.07056-11
SAVRASOVA EKATERINA A., STOYNOVA NATALIYA V.: "Application of leucine dehydrogenase Bcd from Bacillus subtilis for l-valine synthesis in Escherichia coli under microaerobic conditions", HELIYON, vol. 5, no. 4, 1 April 2019 (2019-04-01), GB , pages e01406, XP055866421, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2019.e01406
ZENG, BANGDING ET AL.: "Effects of Brevibacterium Flavum with Directed Mutagenesis of ilvN and Co-Expression of ilvBNC Cluster on L-Valine Production", CIESC JOURNAL, vol. 67, no. 7, 31 July 2016 (2016-07-31), pages 2956 - 2962, XP055866423
VYAZMENSKY, M ET AL., BIOCHEMISTRY, vol. 35, 1996, pages 10339 - 10346
DE FELICE ET AL., JOURNAL OF BACTERIOLOGY, vol. 120, 1974, pages 1058 - 1067
TAN ET AL., APPL ENVIRON MICROBIOL, vol. 79, 2013, pages 4838 - 4844
DATSENKOWANNER, PROC NATL ACAD SCI USA, vol. 97, 2000, pages 6640 - 6645
LU ET AL., APPL MICROBIOL BIOTECHNOL, vol. 93, 2012, pages 2455 - 2462
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 253, 1978, pages 5719 - 5725
BRINKMANN-CHEN, S.CAHN, JKBARNOLD, FH: "Uncovering rare NADH-preferring ketol-acid reductoisomerases", METAB ENG, vol. 26, 2014, pages 17 - 22
See also references of EP 3929297A4
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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