Title:
RECOMBINANT MICROORGANISM FOR PRODUCING THREONINE, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/151411
Kind Code:
A1
Abstract:
The present invention relates to the technical field of microbial engineering, in particular to a recombinant microorganism for producing threonine, and a construction method therefor and the use thereof. In the present invention, an acetate kinase-inactivated strain is constructed and applied to the production of threonine, significantly improving the capability of the strain for producing threonine, further improving the yield of threonine in combination with the enhanced expressions of malate quinone oxidoreductase, glucose-6-phosphate dehydrogenase, 6-phosphate gluconate dehydrogenase, etc., providing a new method for producing threonine on a large scale, and having a high application value.
Inventors:
KANG PEI (CN)
YAO JIAQI (CN)
GONG WEIBO (CN)
HE JUN (CN)
LI YAN (CN)
YAO JIAQI (CN)
GONG WEIBO (CN)
HE JUN (CN)
LI YAN (CN)
Application Number:
PCT/CN2022/143101
Publication Date:
August 17, 2023
Filing Date:
December 29, 2022
Export Citation:
Assignee:
LANGFANG MEIHUA BIOTECHNOLOGY DEV CO LTD (CN)
International Classes:
C12N1/21; C12N15/52; C12N15/53; C12N15/54; C12N15/60; C12N15/77; C12P13/08; C12R1/15
Foreign References:
| US20150329882A1 | 2015-11-19 | |||
| CN113322218A | 2021-08-31 |
Other References:
刘旭峰 (LIU, XUFENG): "干扰合成代谢基因表达对L-苏氨酸合成的影响 (Non-official translation: Effects of Interference with Anabolic Gene Expression on L-threonine Synthesis)", 中国优秀硕士学位论文全文数据库 工程科技I辑 (CHINA MASTER’S THESES FULL-TEXT DATABASE, ENGINEERING SCIENCE & TECHNOLOGY I), no. 7, 15 July 2020 (2020-07-15)
Attorney, Agent or Firm:
BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)
Download PDF: