Title:
RECOMBINANT MICROORGANISM, AND PRODUCTION METHOD OF PYRIDOXAMINE, OR SALT THEREOF, USING SAID RECOMBINANT MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2018/207930
Kind Code:
A1
Abstract:
A recombinant microorganism is provided which has a gene encoding pyridoxine oxidase and a gene encoding pyridoxamine synthetase having enzymatic activity for synthesizing pyridoxamine from pyridoxal, wherein the gene encoding pyridoxine oxidase and the gene encoding pyridoxamine synthetase are each either a gene introduced from outside the cell or an endogenous gene with enhanced expression. Also provided is a method of using the recombinant microorganism to produce pyridoxamine, or a salt thereof, from pyridoxine, or a salt thereof.
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Inventors:
TATENO TOSHIHIRO (JP)
HIDESAKI TOMONORI (JP)
ARAKI TADASHI (JP)
SHINDO ATSUNORI (JP)
MATSUMOTO YOSHIKO (JP)
MIYATA TOSHIO (JP)
HONJO MASARU (JP)
HIDESAKI TOMONORI (JP)
ARAKI TADASHI (JP)
SHINDO ATSUNORI (JP)
MATSUMOTO YOSHIKO (JP)
MIYATA TOSHIO (JP)
HONJO MASARU (JP)
Application Number:
PCT/JP2018/018412
Publication Date:
November 15, 2018
Filing Date:
May 11, 2018
Export Citation:
Assignee:
MITSUI CHEMICALS INC (JP)
RENASCIENCE CO LTD (JP)
RENASCIENCE CO LTD (JP)
International Classes:
C12N15/09; C12N1/21; C12P17/12
Domestic Patent References:
| WO2006066806A1 | 2006-06-29 | |||
| WO2005077902A2 | 2005-08-25 | |||
| WO2007142222A1 | 2007-12-13 | |||
| WO2004035010A2 | 2004-04-29 |
Foreign References:
| JP2009291158A | 2009-12-17 | |||
| JP2014516572A | 2014-07-17 | |||
| JPH09107985A | 1997-04-28 | |||
| JP2000023690A | 2000-01-25 | |||
| JP2017095572A | 2017-06-01 |
Other References:
KANEDA, Y. ET AL.: "Purification, molecular cloning, and characterization of pyridoxine 4- oxidase from Microbacterium luteolum", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 66, no. 5, 2002, pages 1022 - 1031, XP055562583
YOSHIKANE, Y. ET AL.: "Molecular cloning, expression and characterization of pyridoxamine- pyruvate aminotransferase", BIOCHEM. J., vol. 396, 2006, pages 499 - 507, XP055562586
YOSHIKANE, Y. ET AL.: "Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 67, 2010, pages 104 - 110, XP027289897
JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 67, 2010, pages 104 - 110
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 237, no. 1, January 1962 (1962-01-01), pages 127 - 132
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 2, 2008, pages 1120 - 1127
KRAMER, W.FRITA, H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
VAN DEN HONDEL, C.A.M. J.J. ET AL.: "More Gene Manipulations in Fungi.", 1991, ACADEMIC PRESS, pages: 396 - 428
See also references of EP 3623471A4
YOSHIKANE, Y. ET AL.: "Molecular cloning, expression and characterization of pyridoxamine- pyruvate aminotransferase", BIOCHEM. J., vol. 396, 2006, pages 499 - 507, XP055562586
YOSHIKANE, Y. ET AL.: "Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 67, 2010, pages 104 - 110, XP027289897
JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 67, 2010, pages 104 - 110
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 237, no. 1, January 1962 (1962-01-01), pages 127 - 132
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 2, 2008, pages 1120 - 1127
KRAMER, W.FRITA, H.J., METHODS IN ENZYMOLOGY, vol. 154, 1987, pages 350
SAMBROOK, J. ET AL.: "Molecular Cloning A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
VAN DEN HONDEL, C.A.M. J.J. ET AL.: "More Gene Manipulations in Fungi.", 1991, ACADEMIC PRESS, pages: 396 - 428
See also references of EP 3623471A4
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
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