Title:
RECOMBINANT NUCLEOTIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME
Document Type and Number:
WIPO Patent Application WO/2016/172678
Kind Code:
A3
Abstract:
A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MCI and a cytidine-specific recombinant RNase Cusativin.
Inventors:
ADDEPALLI BALASUBRAHMANYAM (US)
LESSNER NICHOLAS PAUL (US)
LIMBACH PATRICK ALAN (US)
VENUS SARAH (US)
LESSNER NICHOLAS PAUL (US)
LIMBACH PATRICK ALAN (US)
VENUS SARAH (US)
Application Number:
PCT/US2016/029151
Publication Date:
December 15, 2016
Filing Date:
April 25, 2016
Export Citation:
Assignee:
UNIV CINCINNATI (US)
International Classes:
C12N9/22; C07H21/04; C07K14/00; C12P21/06; C12Q1/68
Domestic Patent References:
| WO2008103763A2 | 2008-08-28 |
Foreign References:
| US20120167245A1 | 2012-06-28 |
Other References:
NUMATA ET AL.: "Expression and mutational analysis of amino acid residues involved in catalytic activity in a ribonuclease MC 1 from the seeds of bitter gourd.", BIOSCI BIOTECHNOL BIOCHEM., vol. 64, no. 3, 2000, pages 603 - 5, XP055337254
DATABASE GenPept [O] NCBI; 10 October 2012 (2012-10-10), "Chain A, Ribonuclease Mc1 From The Seeds Of Bitter Gourd", XP055337269, Database accession no. 1BK7_A
ROJO ET AL.: "Cusativin, a new cytidine-specific ribonuclease accumulated in seeds of Cucumis sativus L.", PLANTA., vol. 194, no. 3, 1994, pages 328 - 38, XP002936665, ISSN: 0032-0935
DATABASE GenPept [O] NCBI; 10 October 2012 (2012-10-10), "Chain A, Ribonuclease Mc1 From The Seeds Of Bitter Gourd", XP055337269, Database accession no. 1BK7_A
ROJO ET AL.: "Cusativin, a new cytidine-specific ribonuclease accumulated in seeds of Cucumis sativus L.", PLANTA., vol. 194, no. 3, 1994, pages 328 - 38, XP002936665, ISSN: 0032-0935
Attorney, Agent or Firm:
WILLIAMS, Larry, D. et al. (Herron & Evans LLP,2700 Carew Tower,441 Vine Stree, Cincinnati OH, US)
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