Title:
RECOMBINANT ONCOLYTIC INFLUENZA VIRUS PREPARATION METHOD AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2018/120496
Kind Code:
A1
Abstract:
Provided are a preparation method and application of a recombinant oncolytic influenza virus expressing HTRP1 and HTRP2, wherein the HTRP1 is: (A1) a protein represented by SEQ ID No.1; (A2) a protein derived from (A1) by substitution, deletion and/or addtion of one or more amino acid residues in SEQ ID No.1; or (A3) a fusion protein obtained by connecting the N terminal or/and C terminal of (A1) or (A2) with a label; the HTRP2 is: (B1) a protein represented by SEQ ID No.3; (B2) a protein derived from (B1) by substitution, deletion and/or addtion of one or more amino acid residues in SEQ ID No.3; or (B3) a fusion protein obtained by connecting the N terminal or/and C terminal of (B1) or (B2) with a label. The recombinant oncolytic influenza virus can be used in targeting killing of hepatoma cells without influencing normal host cells, and thus can be used in hepatoma targeting therapy.
Inventors:
YANG PENGHUI (CN)
WANG XILIANG (CN)
ZHANG SHAOGENG (CN)
REN TIANYU (CN)
ZHANG PEIRUI (CN)
WANG ZHAOHAI (CN)
SUN FANG (CN)
WANG XILIANG (CN)
ZHANG SHAOGENG (CN)
REN TIANYU (CN)
ZHANG PEIRUI (CN)
WANG ZHAOHAI (CN)
SUN FANG (CN)
Application Number:
PCT/CN2017/080010
Publication Date:
July 05, 2018
Filing Date:
April 11, 2017
Export Citation:
Assignee:
BEIJING 302 HOSPITAL (CN)
International Classes:
A61K35/768; C12N7/01; A61P35/00; C12N15/44; C12N15/62; C12N15/63
Domestic Patent References:
| WO2016109656A1 | 2016-07-07 |
Foreign References:
| CN105754960A | 2016-07-13 | |||
| CN104312983A | 2015-01-28 |
Other References:
YANG XIAORU ET AL.: "Effect of Tyrosine Kinase Inhibitor on aIB-AR induced Ca Influx Involved in hTrp3 protein", CHINESE PHARMACOLOGICAL BULLETIN, vol. 18, no. 2, 30 April 2002 (2002-04-30), pages 178 - 182, ISSN: 1001-1978
YAN YAN ET AL.: "Construction of Reassortant H1N1 Influenza A virus by Eight-plasmid system", JOURNAL OF NORTHWEST A&F UNIVERSITY NATURAL SCIENCE EDITION, vol. 35, no. 9, 30 September 2007 (2007-09-30), pages 15 - 18, ISSN: 1671-9387
CLAY L. EFFERSON: "Prostate Tumor Cells Infected with a Recombinant In- fluenza Virus Expressing a Truncated NS1 Protein Activate Cytolytic CD 8+ Ce- lls To Recognize Noninfected Tumor Cells", JOURNAL OF VIROLOGY, 31 January 2006 (2006-01-31), XP055605490, ISSN: 0022-538X
YAN YAN ET AL.: "Construction of Reassortant H1N1 Influenza A virus by Eight-plasmid system", JOURNAL OF NORTHWEST A&F UNIVERSITY NATURAL SCIENCE EDITION, vol. 35, no. 9, 30 September 2007 (2007-09-30), pages 15 - 18, ISSN: 1671-9387
CLAY L. EFFERSON: "Prostate Tumor Cells Infected with a Recombinant In- fluenza Virus Expressing a Truncated NS1 Protein Activate Cytolytic CD 8+ Ce- lls To Recognize Noninfected Tumor Cells", JOURNAL OF VIROLOGY, 31 January 2006 (2006-01-31), XP055605490, ISSN: 0022-538X
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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