Title:
RECOMBINANT PHYCOBILIPROTEIN AND PHYCOBILIPROTEIN LINKER FUSION PROTEINS AND USES THEREFORE
Document Type and Number:
WIPO Patent Application WO2001096871
Kind Code:
A3
Abstract:
This invention is directed to the utilization of the developing methods for molecular manipulation of cyanobacteria and red algae (and potentially cryptomonad algae) to express of phycobiliproteins and phycobiliprotein linker fusion proteins and their utilization as phycobiliprotein, phycobilisome and subassembly based reagents. In particular, the present invention relates to a method for a specific binding assay to determine a target moiety which is a member of a specific binding pair, and provides an improvement in the method comprising using a detectable label which is a fusion protein containing both a phycobiliprotein domain and another domain corresponding to a first member of a specific binding pair, where the fusion protein binds to a second member of the specific binding pair to provide a detectable labeled complex. The domain derived from the first member of the specific binding pair can be directly fused to the phycobiliprotein or phycobiliprotein linker domain or be separated by a spacer that allows correct folding of both domains.
Inventors:
ALLNUTT F C THOMAS (US)
TOOLE COLLEEN M (US)
MORSEMAN JOHN P (US)
TOOLE COLLEEN M (US)
MORSEMAN JOHN P (US)
Application Number:
PCT/US2001/019439
Publication Date:
April 11, 2002
Filing Date:
June 18, 2001
Export Citation:
Assignee:
MARTEK BIOSCIENCES CORP (US)
ALLNUTT F C THOMAS (US)
TOOLE COLLEEN M (US)
MORSEMAN JOHN P (US)
ALLNUTT F C THOMAS (US)
TOOLE COLLEEN M (US)
MORSEMAN JOHN P (US)
International Classes:
C07K14/195; C07K14/405; G01N33/533; G01N33/542; (IPC1-7): G01N33/542; C12N15/62
Domestic Patent References:
| WO2001046395A1 | 2001-06-28 |
Other References:
CAI YUPING A ET AL: "Recombinant C-phycocyanins equipped with affinity tags, oligomerization, and biospecific recognition domains.", ANALYTICAL BIOCHEMISTRY, vol. 290, no. 2, 15 March 2001 (2001-03-15), pages 186 - 204, XP002185513, ISSN: 0003-2697
CHERRY RICHARD J ET AL: "Detection of dimers of dimers of human leukocyte antigen (HLA)-DR on the surface of living cells by single-particle fluorescence imaging.", JOURNAL OF CELL BIOLOGY, vol. 140, no. 1, 12 January 1998 (1998-01-12), pages 71 - 79, XP002185514, ISSN: 0021-9525
CHERRY RICHARD J ET AL: "Detection of dimers of dimers of human leukocyte antigen (HLA)-DR on the surface of living cells by single-particle fluorescence imaging.", JOURNAL OF CELL BIOLOGY, vol. 140, no. 1, 12 January 1998 (1998-01-12), pages 71 - 79, XP002185514, ISSN: 0021-9525
Download PDF: