RECOMBINANT PROTEIN CARRYING TARGET PROTEIN AND AUTONOMOUSLY ENTERING EUKARYOTIC CELL, RECOMBINANT EXPRESSION VECTOR, RECOMBINANT BACTERIUM, AND USE

WIPO Patent Application WO/2023/197365

A1

The present invention relates to the technical field of fusion proteins. Provided are a recombinant protein carrying a target protein and autonomously entering a eukaryotic cell, a recombinant expression vector, a recombinant bacterium, and the use. The recombinant protein sequentially comprises, from the N-terminus to the C-terminus, a nuclear localization signal (NLS) that carries the recombinant protein into the nucleus, a target protein and short peptide AE1 having an ability of spanning the cell membrane, wherein AE1 nontoxically and efficiently guides the recombinant protein to span the cell membrane and enter a cell, and the target protein is carried into the nucleus by means of the NLS, thereby playing a role. Also provided is a recombinant photolyase capable of autonomously entering a human nucleus to repair DNA damage induced by UV radiation. The CPD photolyase used as a target protein has the effects of repairing a cyclobutane pyrimidine dimer (CPD) on the nuclear DNA, improving the activity of intracellular superoxide dismutase (SOD), and reducing the levels of intracellular reactive oxygen species (ROS) and a highly toxic molecule malondialdehyde (MDA).

FANG WEIGUO (CN)
BAO YUTING (CN)
ZHANG MINGXIANG (CN)

PCT/CN2022/088658

October 19, 2023

April 24, 2022

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UNIV ZHEJIANG (CN)

A61K38/51; C07K19/00; A61P17/16; A61P17/18; C12N1/21; C12N9/88; C12N15/70; C12R1/19

US20200283745A1	2020-09-10
US20030229202A1	2003-12-11
US20090130034A1	2009-05-21
US20050014263A1	2005-01-20
US20090054828A1	2009-02-26
CN113454103A	2021-09-28
CN111936624A	2020-11-13

BEIJING FINELAND IP FIRM (CN)

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