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Title:
RECOMBINANT PROTEIN OF FIBROBLAST GROWTH FACTOR HAVING ADHESIVE ACTIVITY FOR STEM CELLS AND METHOD FOR CULTURING STEM CELLS USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2010/131821
Kind Code:
A1
Abstract:
The present invention relates to a recombinant protein of a fibroblast growth factor (FGF) having an adhesive activity for stem cells and a method for culturing stem cells using the same. More particularly, the present invention relates to a recombinant protein having an adhesive activity for stem cells by fusion of a polypeptide linker at amino terminal of FGF, and a method for culturing stem cells using immobilized FGF comprising: fixing the recombinant protein in a culture vessel with a hydrophobic surface using amino terminal of the polypeptide linker, adhering stem cells on the recombinant protein-fixed culture vessel, and culturing the stem cells.

Inventors:
KIM SANG-HEON (KR)
KIM SOO HYUN (KR)
JUNG YOUNG MEE (KR)
HAN MIN (KR)
Application Number:
PCT/KR2009/005899
Publication Date:
November 18, 2010
Filing Date:
October 14, 2009
Export Citation:
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Assignee:
KOREA INST SCI & TECH (KR)
KIM SANG-HEON (KR)
KIM SOO HYUN (KR)
JUNG YOUNG MEE (KR)
HAN MIN (KR)
International Classes:
C12N5/074
Foreign References:
US20070269886A12007-11-22
EP2048227A22009-04-15
Other References:
HATTORI, H. ET AL.: "Expansion and Characterization of Adipose Tissue-derived Stromal Cells cultured with Low Serum Medium.", J. BIOMED. MATER. RES. PART B: APPL. BIOMATER., vol. 87, 21 May 2008 (2008-05-21), pages 229 - 236
DE HAAN, G. ET AL.: "In Vitro Generation of Long-Term Repopulating Hematopoietic Stem Cells.", DEVELOPMENTAL CELL, vol. 4, February 2003 (2003-02-01), pages 241 - 251
LEMAITRE, G. ET AL.: "Production and purification of active FGF2 via recombinant fusion protein.", BIOCHIMIE, vol. 77, 1995, pages 162 - 166
Attorney, Agent or Firm:
CHANG, Soo Kil et al. (Seyang B/D223 Naeja-dong, Jongno-gu, Seoul 110-720, KR)
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Claims: