Title:
RECOMBINANT PROTEIN HAVING TRANSGLUTAMINASE ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2018/004014
Kind Code:
A1
Abstract:
The invention provides an active microbial transglutaminase (MTG) that does not require protease treatment in a protein expression system using a universal host such as Escherichia coli, that is, an MTG mutant having transglutaminase activity, and a method for producing the same. The invention is a protein obtained by mutating an amino acid in the amino acid sequence of the propeptide moiety in wild-type microbial transglutaminase, wherein the protein is characterized by having transglutaminase activity.
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Inventors:
KAMIYA NORIHO (JP)
HAYASHI KONOSUKE (JP)
MINAMIHATA KOSUKE (JP)
MATSUZAKI TAKASHI (JP)
HAYASHI KONOSUKE (JP)
MINAMIHATA KOSUKE (JP)
MATSUZAKI TAKASHI (JP)
Application Number:
PCT/JP2017/024881
Publication Date:
January 04, 2018
Filing Date:
June 30, 2017
Export Citation:
Assignee:
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N9/10; C12N1/21; C12N15/09
Domestic Patent References:
| WO2009030211A2 | 2009-03-12 |
Foreign References:
| JP2009504170A | 2009-02-05 |
Other References:
RICKERT M. ET AL.: "Production of soluble and active microbial transglutaminase in Escherichia coli for site-specific antibody drug conjugation", PROTEIN SCI., vol. 25, February 2016 (2016-02-01), pages 442 - 455, XP055276114, DOI: doi:10.1002/pro.2833
CHEN K. ET AL.: "Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide", J. IND. MICROBIOL. BIOTECHNOL., vol. 40, no. 3, 2013, pages 317 - 325, XP035330676, DOI: 10.1007/s10295-012-1221-y
MATSUZAKI, TAKASHI ET AL.: "Shinki Biseibutsu Yurai Transglutaminase Sosei ni Muketa Kiso Kenkyu", DAI 53 KAI PREPRINTS OF JOINT CHEMICAL CONFERENCE OF KYUSHU BRANCH GAIKOKUJIN KENKYUSHA KORYU KOKUSAI SYMPOSIUM, 2 July 2016 (2016-07-02), pages 17
CHEN K. ET AL.: "Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide", J. IND. MICROBIOL. BIOTECHNOL., vol. 40, no. 3, 2013, pages 317 - 325, XP035330676, DOI: 10.1007/s10295-012-1221-y
MATSUZAKI, TAKASHI ET AL.: "Shinki Biseibutsu Yurai Transglutaminase Sosei ni Muketa Kiso Kenkyu", DAI 53 KAI PREPRINTS OF JOINT CHEMICAL CONFERENCE OF KYUSHU BRANCH GAIKOKUJIN KENKYUSHA KORYU KOKUSAI SYMPOSIUM, 2 July 2016 (2016-07-02), pages 17
Attorney, Agent or Firm:
KOBAYASHI, Hiroshi et al. (JP)
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