Title:
RECOMBINANT PSEUDOMONAS PLECOGLOSSICIDA FOR PRODUCING L-XYLOSE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/037998
Kind Code:
A1
Abstract:
Provided are a recombinant pseudomonas plecoglossicida for producing L-xylose and an application thereof. The recombinant bacterium expresses a 2-keto gluconate reductase gene, a 2,5-diketo gluconate reductase gene, and a pyruvate decarboxylase gene which are successfully expressed in a host pseudomonas plecoglossicida by means of a double-plasmid system; an obtained strain is fermented for 56 h on a shake flask, the yield of L-xylose can reach 16.2 g/L, and the conversion rate reaches 20.3%; the strain is fermented for 48 h and 44 h on a 3L and 15L fermentation tank, respectively, the yield of L-xylose can reach 37.6 g/L and 45.8 g/L, respectively, and the glucose conversion rate is 47.0% and 57.3%, respectively.
Inventors:
LU XI (CN)
Application Number:
PCT/CN2019/082860
Publication Date:
February 27, 2020
Filing Date:
April 16, 2019
Export Citation:
Assignee:
SHANGHAI LINKCHEM TECH CO LTD (CN)
International Classes:
C12N1/21; C12N15/78; C12P19/02; C12R1/38
Domestic Patent References:
| WO2005017099A2 | 2005-02-24 |
Foreign References:
| CN109055292A | 2018-12-21 | |||
| CN107312766A | 2017-11-03 | |||
| CN108165591A | 2018-06-15 |
Other References:
ARUN GUPTA: "Gluconobacter oxydans: Its Biotechnological Applications", J. MOL. MICROBIOL. BIOTECHNOL., vol. 3, 31 July 2001 (2001-07-31), pages 3, XP008026573, ISSN: 1464-1801
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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