Title:
RECOMBINANT STRAIN OF SACCHAROMYCES CEREVISIAE OVERPRODUCING GLYCEROL
Document Type and Number:
WIPO Patent Application WO/2015/088892
Kind Code:
A3
Abstract:
A truncated version of Saccharomyces cerevisiae IVL2 gene encoding a cytosolic form of acetolactate synthase was cloned into an expression cassette under control of a strong constitutive alcohol dehydrogenase (ADH1) promoter. The plasmid was introduced into the S. cerevisiae strain and the recombinant strain was tested for ability to overproduce glycerol under anaerobic conditions. It was shown that the recombinant strain was characterized by increased glycerol production and decreased ethanol production under anaerobic conditions.
Inventors:
SIBIRNY ANDRIY (UA)
DMYTRUK KOSTYANTYN V (UA)
ABBAS CHARLES (US)
MURASHCHENKO LIDIIA R (UA)
DMYTRUK KOSTYANTYN V (UA)
ABBAS CHARLES (US)
MURASHCHENKO LIDIIA R (UA)
Application Number:
PCT/US2014/068699
Publication Date:
August 13, 2015
Filing Date:
December 05, 2014
Export Citation:
Assignee:
ARCHER DANIELS MIDLAND CO (US)
International Classes:
C12P7/20
Domestic Patent References:
| WO2011040901A2 | 2011-04-07 | |||
| WO2013076144A2 | 2013-05-30 |
Foreign References:
| US8232089B2 | 2012-07-31 | |||
| US20100167364A1 | 2010-07-01 | |||
| US8455239B2 | 2013-06-04 |
Other References:
TAXIS ET AL.: "System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae", BIOTECHNIQUES, vol. 40, no. 1, 1 January 2006 (2006-01-01), pages 73 - 78, XP001537899
Attorney, Agent or Firm:
CRAFTON, Corey et al. (4666 Faries ParkwayDecatur, Illinois, US)
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