Title:
RECOMBINANT YEAST FOR PRODUCING 2,3-BUTANEDIOL INTO WHICH CANDIDA TROPICALIS-DERIVED PYRUVATE DECARBOXYLASE IS INTRODUCED, AND METHOD FOR PRODUCING 2,3-BUTANEDIOL BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2018/212366
Kind Code:
A1
Abstract:
The present invention relates to a recombinant yeast for producing 2,3-butanediol and a method for producing 2,3-butanediol by using the same. The present invention introduces, into a yeast, Candida tropicalis-derived pyruvate decarboxylase having lower activity than pyruvate decarboxylase, which is possessed by the yeast itself, such that acetyl-CoA synthesis is possible even if ethanol acting as a by-product in 2,3-butanediol production is not produced, thereby enabling the growth rate of the yeast and the substrate consumption rate to increase. Therefore, the present invention ultimately can greatly improve the productivity of 2,3-butanediol. The present invention relates to a method for producing 2,3-butanediol. A conventional technique for producing 2,3-butanediol by using Saccharomyces cerevisiae (yeast) allows the production of a large amount of glycerol as a by-product together with the production of 2,3-butanediol. However, the yeast strain of the present invention enables the production of high-purity 2,3-butanediol with high yield and high productivity in a state in which the production of glycerol, which is a by-product, is inhibited.
Inventors:
SEO JIN HO (KR)
KIM JIN WOO (KR)
KIM JIN WOO (KR)
Application Number:
PCT/KR2017/005008
Publication Date:
November 22, 2018
Filing Date:
May 15, 2017
Export Citation:
Assignee:
SEOUL NAT UNIV R&DB FOUNDATION (KR)
International Classes:
C12N15/81; C12P7/16
Foreign References:
| KR20170028055A | 2017-03-13 | |||
| KR101590993B1 | 2016-02-02 | |||
| KR20160093492A | 2016-08-08 |
Other References:
KIM, S. ET AL.: "Efficient Production of 2,3-butanediol in Saccharomyces Cerevisiae by Eliminating Ethanol and Glycerol Production and Redox Rebalancing", METABOLIC ENGINEERING, vol. 31, 2015, pages 94 - 101, XP055212598, DOI: doi:10.1016/j.ymben.2015.07.006
KIM, S. ET AL.: "Metabolic Engineering of Saccharomyces Cerevisiae for 2,3-butanediol Production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 101, no. 6, 15 February 2017 (2017-02-15), pages 2241 - 2250, XP036164240, DOI: 10.1007/s00253-017-8172-1
See also references of EP 3505633A4
KIM, S. ET AL.: "Metabolic Engineering of Saccharomyces Cerevisiae for 2,3-butanediol Production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 101, no. 6, 15 February 2017 (2017-02-15), pages 2241 - 2250, XP036164240, DOI: 10.1007/s00253-017-8172-1
See also references of EP 3505633A4
Attorney, Agent or Firm:
TAEDONG INTERNATIONAL PATENT&LAW FIRM (KR)
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