Title:
RECOMBINASE GENOME EDITING
Document Type and Number:
WIPO Patent Application WO/2017/184227
Kind Code:
A3
Abstract:
A method of altering a target nucleic acid sequence within a cell is provided including providing the cell with a donor nucleic acid, providing the cell with a single strand annealing protein, and providing the cell with a single strand DNA binding protein, wherein one or more or both of the single strand annealing protein and the single strand DNA binding protein is foreign to the cell, and wherein the donor nucleic acid is recombined into the target nucleic acid.
Inventors:
CHURCH GEORGE M (US)
GREGG CHRISTOPHER J (US)
LAJOIE MARC J (US)
RIOS XAVIER (US)
GREGG CHRISTOPHER J (US)
LAJOIE MARC J (US)
RIOS XAVIER (US)
Application Number:
PCT/US2017/016184
Publication Date:
February 08, 2018
Filing Date:
February 02, 2017
Export Citation:
Assignee:
HARVARD COLLEGE (US)
International Classes:
C12N15/90; C12N1/19; C12N1/21; C12N15/74; C12N15/79; C12N15/81
Foreign References:
| US7144734B2 | 2006-12-05 |
Other References:
ANTONY ET AL.: "Multiple C-terminal tails within a single E. coli SSB homotetramer coordinate DNA replication and repair", J MOL BIO, vol. 425, no. 23, 2013, pages 4802 - 4819, XP028766573
DATABASE GenBank [O] 2 April 2015 (2015-04-02), "single-stranded DNA-binding protein [Escherichia coli K-12]", XP055462012, Database accession no. CQR83440.1.
MEYER ET AL.: "The single-stranded DNA-binding protein of Escherichia coli", MICROBIOL REV, vol. 54, no. 4, 1990, pages 342 - 380, XP055462010
DATABASE GenBank [O] 26 November 2012 (2012-11-26), "hypothetical protein PA39016_003090055 [Pseudomonas aeruginosa 39016]", XP055462020, Database accession no. ZP 07797103.1
DATABASE GenBank 8 February 2016 (2016-02-08), "phage recombination protein Bet [Pseudomonas aeruginosa]", XP055462028, Database accession no. WP_003088368.1
DATABASE GenBank [O] 2 April 2015 (2015-04-02), "single-stranded DNA-binding protein [Escherichia coli K-12]", XP055462012, Database accession no. CQR83440.1.
MEYER ET AL.: "The single-stranded DNA-binding protein of Escherichia coli", MICROBIOL REV, vol. 54, no. 4, 1990, pages 342 - 380, XP055462010
DATABASE GenBank [O] 26 November 2012 (2012-11-26), "hypothetical protein PA39016_003090055 [Pseudomonas aeruginosa 39016]", XP055462020, Database accession no. ZP 07797103.1
DATABASE GenBank 8 February 2016 (2016-02-08), "phage recombination protein Bet [Pseudomonas aeruginosa]", XP055462028, Database accession no. WP_003088368.1
Attorney, Agent or Firm:
IWANICKI, John P. (US)
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