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Title:
RECOMBINASE POLYMERASE AMPLIFICATION REAGENT KIT, AMPLIFICATION METHOD, AND AMPLIFICATION REAGENT
Document Type and Number:
WIPO Patent Application WO/2018/014799
Kind Code:
A1
Abstract:
A recombinase polymerase amplification reagent kit, comprising: E. coli RecA protein; λphage Orf protein; E. coli SSB protein, and DNA polymerase; also provided are a recombinase polymerase amplification method and amplification reagent.

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Inventors:
SHENG SITONG (CN)
GONG JINGWEN (CN)
Application Number:
PCT/CN2017/093088
Publication Date:
January 25, 2018
Filing Date:
July 17, 2017
Export Citation:
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Assignee:
GUANGZHOU CONSIDERIN GENE & HEALTH TECH CO LTD (CN)
International Classes:
C12Q1/68
Domestic Patent References:
WO2008035205A22008-03-27
Foreign References:
CN104694662A2015-06-10
CN105567866A2016-05-11
Other References:
CURTIS, F. A.: "The C-terminus of the phage X Orf recombinase is involved in DNA binding", JOURNAL OF MOLECULAR RECOGNITION, vol. 24, 13 December 2010 (2010-12-13)
CURTIS, F. A.: "Phage Orf Family Recombinases: Conservation of Activities and Involvement of the Central Channel in DNA Binding", PLOS ONE, vol. 9, no. 8, 1 August 2014 (2014-08-01)
GAO, WEIFANG ET AL.: "Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 32, no. 6, 30 June 2016 (2016-06-30), pages 627 - 634
JING, ZHIGANG ET AL.: "Research Progress on Recombinase Polymerase Amplification", BIOTECHNOLOGY BULLETIN, vol. 32, no. 6, 26 June 2016 (2016-06-26), pages 47 - 53
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