RECOMBINATION SYSTEMS FOR HIGH-THROUGHPUT CHROMOSOMAL ENGINEERING OF BACTERIA

Title:

RECOMBINATION SYSTEMS FOR HIGH-THROUGHPUT CHROMOSOMAL ENGINEERING OF BACTERIA

Document Type and Number:

WIPO Patent Application WO/2019/140328

Kind Code:

A1

Abstract:

In some aspects, the disclosure relates methods and compositions for genetic engineering of cells (e.g., bacterial cells, etc.). The disclosure is based, in part, on a combination of genetic recombination systems (e.g., a "targeting oligonucleotide" and a "payload plasmid) that enable high-throughput chromosomal engineering and do not involve the preparation of double-stranded DNA (dsDNA) recombination substrates. In some aspects, the disclosure provides engineered bacterial cells comprising a targeting oligonucleotide and a payload plasmid.

Inventors:

MURPHY KENAN (US)
SASSETTI CHRISTOPHER (US)

Application Number:

PCT/US2019/013381

Publication Date:

July 18, 2019

Filing Date:

January 11, 2019

Export Citation:

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Assignee:

UNIV MASSACHUSETTS (US)

International Classes:

C12N15/09; C12N1/20; C12N15/63; C12N15/74; C12N15/90; C12R1/32; C12R1/34

Domestic Patent References:

WO2017127612A1	2017-07-27
WO2017075529A1	2017-05-04

Foreign References:

US20060046294A1	2006-03-02
US20130034882A1	2013-02-07
US6294328B1	2001-09-25

Other References:

DATABASE Nucleotide 5 October 2017 (2017-10-05), SHEEN, P ET AL.: "Mycobacterium tuberculosis strain TBDM2189 chromosome, complete genome", XP055625033, retrieved from ncbi Database accession no. CP023632.1
MURPHY, K ET AL.: "ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes", MBIO, vol. 9, no. 6, 11 December 2018 (2018-12-11), pages e01467-18, XP055625034
See also references of EP 3737754A4

Attorney, Agent or Firm:

MACDONALD, Kevin (US)

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