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Title:
REDUCTION OF NON-STARCH POLYSACCHARIDES AND ALPHA-GALACTOSIDES IN SOY FLOUR BY MEANS OF SOLID-STATE FERMENTATION USING CELLULOLYTIC BACTERIA ISOLATED FROM DIFFERENT ENVIRONMENTS
Document Type and Number:
WIPO Patent Application WO/2013/114282
Kind Code:
A3
Abstract:
The invention relates to a method for fermenting soy flour in the solid state in order to reduce non-starch polysaccharides and alpha-galactosides, said method comprising the following steps: a) preparation of the fermentation substrate; b) inoculation of the substrate with selected celluloytic bacterial strains; c) incubation; and, optionally, d) drying of the product, which generates a product with: an increase in protein of between 12 and 15% compared to non-fermented soy flour, degradation of the alpha-galactosides of more than 90% compared to non-fermented soy flour, a reduction in non-starch polysaccharides (NSPs) of between 15 and 25%, an amino acid profile similar to that of non-fermented soy flour, and immune-stimulating effects.

Inventors:
ROMERO ORMAZÁBAL, Jaime Moisés (Lord Cochrane 298, Depto 802 Santiag, Santiago 02, 83304, CL)
OPAZO SALAS, Rafael José Daniel (El Líbano 5524, MaculSantiago, 90, 78304, CL)
Application Number:
IB2013/050763
Publication Date:
August 08, 2013
Filing Date:
January 29, 2013
Export Citation:
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Assignee:
UNIVERSIDAD DE CHILE (Av. Libertador Bernardo O'Higgins 1058, SantiagoSantiago, 11, 83301, CL)
International Classes:
A23L1/20; A23J1/14; A23K1/14; A23K1/18; A23L1/211; C12N1/20; C12P39/00
Foreign References:
DD278058A11990-04-25
US6159715A2000-12-12
KR20100107966A2010-10-06
Other References:
OPAZO, R. ET AL.: "Reduction of soybean meal non-starch polysaccharides and alfa-galactosides by solid-state fermentation using cellulolytic bacteria obteained from differente enviroments.", PLOS ONE., vol. 7, no. 9, September 2012 (2012-09-01), pages E44783, XP055160442
REFSTIE S ET AL.: "Lactic acid fermentation eliminates indigestible carbohydrates and antinutritional factors in soybean meal for Atlantic salmon (Salmo salar).", AQUACULTURE, vol. 246, no. 1-4, 18 May 2005 (2005-05-18), AMSTERDAM, NL, pages 331 - 345, XP027614591
CHOCT, M. ET AL.: "Soy oligosaccharides and soluble non-starch polysaccharides: a review of digestion, nutritive and anti-nutritive effects in pigs and poultry.", ASIAN-AUST. J. ANIM. SCI., vol. 23, no. 10, 8 December 2010 (2010-12-08), pages 1386 - 1398, XP055160445
SHIRATORI, H. ET AL.: "Cohnella fontinalis sp. nov., a xylanolytic bacterium isolated from fresh water.", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 60, 2010, pages 1344 - 1348, XP055160454
GRAMINHA E B N ET AL.: "Enzyme production by solid-state fermentation: Application to animal nutrition.", ANIMAL FEED SCIENCE AND TECHNOLOGY, vol. 144, no. 1-2, 23 June 2008 (2008-06-23), AMSTERDAM, NL, pages 1 - 22, XP022710177
CAO Y ET AL.: "Properties of a novel alpha-galactosidase from Streptomyces sp. S27 and its potential for soybean processing.", ENZYME AND MICROBIAL TECHNOLOGY, vol. 47, no. 7, 8 December 2010 (2010-12-08), STONEHAM, MA US, pages 305 - 312, XP027418039
See also references of EP 2810563A4
Attorney, Agent or Firm:
M&B PROPIEDAD INTELECTUAL E INDUSTRIAL LTDA. et al. (Av. Isidora Goyenechea 3477, Piso 19 Las Conde, Santiago 06, 75501, CL)
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