Title:
REGULATION OF STOMATAL APERTURES BY APYRASES AND EXTRACELLULAR NUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO/2012/167023
Kind Code:
A3
Abstract:
The role of extracellular nucleotides and apyrase enzymes in the guard cells that border stomata in regulating stomatal aperture and the plant's resistance to drought and pathogens is disclosed herein. Expression of apyrases APY1 and APY2, in guard cell protoplasts is strongly correlated with cell growth, cell secretory activity and with conditions that favor stomatal opening. Both short-term inhibition of ectoapyrase activity and long-term suppression of APY1 and APY2 transcript levels significantly disrupt normal stomatal behavior in light. Furthermore, two punnoceptor inhibitors in mammals, pyridoxalphosphate-6-azo-phenyl-2', 4 '-disulphonic acid (PPADS) and Reactive Blue 2, block ATPS- and ADPβS-induced opening and closing, and also partially block the ability of abscisic acid (ABA) to induce stomatal closure, and light-induced stomatal opening. Treatment of epidermal peels with ATPyS induces increased levels of nitric oxide and reactive oxygen species, and genetically suppressing the synthesis of these agents blocks the effects of nucleotides on stomatal aperture.
Inventors:
ROUX STANLEY J (US)
CLARK GREGORY B (US)
STEINEBRUNNER IRIS (DE)
CLARK GREGORY B (US)
STEINEBRUNNER IRIS (DE)
Application Number:
PCT/US2012/040382
Publication Date:
April 25, 2013
Filing Date:
June 01, 2012
Export Citation:
Assignee:
UNIV TEXAS (US)
ROUX STANLEY J (US)
CLARK GREGORY B (US)
STEINEBRUNNER IRIS (DE)
ROUX STANLEY J (US)
CLARK GREGORY B (US)
STEINEBRUNNER IRIS (DE)
International Classes:
A01N63/00; A01G7/00; A01H1/00; A01N63/02; A01P21/00; C12N15/82
Domestic Patent References:
| WO2010065725A2 | 2010-06-10 |
Foreign References:
| US20080058211A1 | 2008-03-06 |
Other References:
CLARK, GREG ET AL.: "EXTRACELLULAR NUCLEOTIDES AND APYRASES REGULATE STOMATAL APERTURE IN ARABIDOPSIS", PLANT PHYSIOLOGY, vol. 156, no. 4, August 2011 (2011-08-01), pages 1740 - 1753, XP055064377
CLARK, GREG. ET AL.: "APYRASE(NUCLEOSIDE TRIPHOSPHATE-DIPHOSPHOHYDROLASE) AND EXTRACELLULAR NUCLEOTIDE REGULATE COTTON FIBER ELONGATION IN CULTURED OVULES", PLANT PHYSIOLOGY, vol. 152, no. 2, 16 December 2009 (2009-12-16), pages 1073 - 1083, XP055064379
CLARK, GREG. ET AL.: "APYRASE(NUCLEOSIDE TRIPHOSPHATE-DIPHOSPHOHYDROLASE) AND EXTRACELLULAR NUCLEOTIDE REGULATE COTTON FIBER ELONGATION IN CULTURED OVULES", PLANT PHYSIOLOGY, vol. 152, no. 2, 16 December 2009 (2009-12-16), pages 1073 - 1083, XP055064379
Attorney, Agent or Firm:
FLORES, Edwin, S. et al. (LLP14951 North Dallas Parkway,Suite 40, Dallas TX, US)
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