Title:
REPLICATION COMPETENT VIRUSES CAPABLE OF SILENCING VIRUS INHIBITORY FACTOR EXPRESSION
Document Type and Number:
WIPO Patent Application WO2005100576
Kind Code:
A3
Abstract:
Described is a replication competent virus, being capable to replicate and having lytic capacity in tarjet cells, the virus comprising in the genome thereof, at least one DNA sequence coding for a silencing factor functional in reducing expression of a target gene in the said target cells, operably linked to one or more expression control sequences, funcional in the said target cells, and the use thereof in the preparation of a medicament and to the use thereof in a method for lysing target cells expressing a virus inhibitory factor.
Inventors:
CARETTE JAN EDUARD (NL)
VAN BEUSECHEM VICTOR WILLEM (NL)
VAN BEUSECHEM VICTOR WILLEM (NL)
Application Number:
PCT/EP2005/004152
Publication Date:
April 13, 2006
Filing Date:
April 15, 2005
Export Citation:
Assignee:
VERENIGING VOOR CHRISTELIJK HOGER ONDERWIJS WETENSCHAPPELIJK ONDERZOEK EN PATIENTENZORG (NL)
CARETTE JAN EDUARD (NL)
VAN BEUSECHEM VICTOR WILLEM (NL)
CARETTE JAN EDUARD (NL)
VAN BEUSECHEM VICTOR WILLEM (NL)
International Classes:
C12N15/861; A61K48/00; C12N15/11; C12N15/113; A61K35/13
Domestic Patent References:
| WO2004013355A1 | 2004-02-12 | |||
| WO2003057892A2 | 2003-07-17 |
Foreign References:
| EP1439227A1 | 2004-07-21 |
Other References:
ARTS GERT-JAN ET AL: "Adenoviral vectors expressing siRNAs for discovery and validation of gene function.", GENOME RESEARCH, vol. 13, no. 10, October 2003 (2003-10-01), pages 2325 - 2332, XP002297535, ISSN: 1088-9051
PADDISON PATRICK J ET AL: "Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells", GENES AND DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, NEW YORK, US, vol. 16, no. 8, 15 April 2002 (2002-04-15), pages 948 - 958, XP002204653, ISSN: 0890-9369
VICKERS TIMOTHY A ET AL: "Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents: A comparative analysis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 278, no. 9, 28 February 2003 (2003-02-28), pages 7108 - 7118, XP002283905, ISSN: 0021-9258
CARETTE JAN E ET AL: "Conditionally replicating adenoviruses expressing short hairpin RNAs silence the expression of a target gene in cancer cells", CANCER RESEARCH, vol. 64, no. 8, 15 April 2004 (2004-04-15), pages 2663 - 2667, XP002297536, ISSN: 0008-5472
UCHIDA HIROAKI ET AL: "Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.", MOLECULAR THERAPY : THE JOURNAL OF THE AMERICAN SOCIETY OF GENE THERAPY. JUL 2004, vol. 10, no. 1, July 2004 (2004-07-01), pages 162 - 171, XP002333253, ISSN: 1525-0016
PADDISON PATRICK J ET AL: "Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells", GENES AND DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, NEW YORK, US, vol. 16, no. 8, 15 April 2002 (2002-04-15), pages 948 - 958, XP002204653, ISSN: 0890-9369
VICKERS TIMOTHY A ET AL: "Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents: A comparative analysis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 278, no. 9, 28 February 2003 (2003-02-28), pages 7108 - 7118, XP002283905, ISSN: 0021-9258
CARETTE JAN E ET AL: "Conditionally replicating adenoviruses expressing short hairpin RNAs silence the expression of a target gene in cancer cells", CANCER RESEARCH, vol. 64, no. 8, 15 April 2004 (2004-04-15), pages 2663 - 2667, XP002297536, ISSN: 0008-5472
UCHIDA HIROAKI ET AL: "Adenovirus-mediated transfer of siRNA against survivin induced apoptosis and attenuated tumor cell growth in vitro and in vivo.", MOLECULAR THERAPY : THE JOURNAL OF THE AMERICAN SOCIETY OF GENE THERAPY. JUL 2004, vol. 10, no. 1, July 2004 (2004-07-01), pages 162 - 171, XP002333253, ISSN: 1525-0016
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