Title:
REPRESENTATION AND APPLICATION OF NOVEL HIGH TEMPERATURE ARGONAUTE PROTEIN TPSAGO
Document Type and Number:
WIPO Patent Application WO/2022/228351
Kind Code:
A1
Abstract:
Provided are a representation and application of a novel high temperature Argonaute protein. Specifically, provided is a nucleic acid cleaving system, which is characterized in that the nucleic acid cleaving system comprises: (a) a guide DNA (gDNA); (b) a programmable endonuclease Argonaute (Ago); and (c) optional reporter nucleic acid, wherein if the reporter nucleic acid is cleaved, the cleavage can be detected. In addition, further provided are a system and a method for enriching and detecting low-abundance target nucleic acids of the programmable endonuclease TpsAgo. The provided detection system and method can specifically target low-abundance nucleic acids for effective enrichment and efficient detection, and the programmable endonuclease TpsAgo has a strong potential for gene manipulation.
Inventors:
FENG YAN (CN)
SUN YINGYING (CN)
GUO XIANG (CN)
YE XINGYU (CN)
LI ZHONGLEI (CN)
SUN YINGYING (CN)
GUO XIANG (CN)
YE XINGYU (CN)
LI ZHONGLEI (CN)
Application Number:
PCT/CN2022/088816
Publication Date:
November 03, 2022
Filing Date:
April 24, 2022
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12Q1/6816; C12N9/22; C12N15/55; C12Q1/6806
Foreign References:
| CN113201578A | 2021-08-03 | |||
| CN111926117A | 2020-11-13 | |||
| CN109880891A | 2019-06-14 | |||
| CN108796036A | 2018-11-13 | |||
| CN110229799A | 2019-09-13 |
Other References:
WU JINEN; YANG JING; CHO WILLIAM C.; ZHENG YADONG: "Argonaute proteins: Structural features, functions and emerging roles", JOURNAL OF ADVANCED RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 24, 29 April 2020 (2020-04-29), AMSTERDAM, NL , pages 317 - 324, XP086216434, ISSN: 2090-1232, DOI: 10.1016/j.jare.2020.04.017
Attorney, Agent or Firm:
XU&PARTNERS, LLC. (CN)
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