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Title:
REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY
Document Type and Number:
WIPO Patent Application WO/2012/108672
Kind Code:
A2
Abstract:
The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.

Inventors:
PARK, Han Oh (208-601, Expo Apt. Jeonmin-dong, Yuseong-gu, Daejeon 305-761, KR)
YANG, Sung Jun (102-603, Dasarang Apt. Moksang-dong, Daedeok-gu, Daejeon 306-760, KR)
JOO, Sung Mo (1 Samsung Apt, Oryu-dong Jung-gu, Daejeon 301-787, 4-1306, KR)
HWANG, Byoung Oh (1 LG Deokdong Apt, Bijeon 2-don, Pyeongtaek-si Gyeonggi-do 450-729, 05-1501, KR)
Application Number:
KR2012/000894
Publication Date:
August 16, 2012
Filing Date:
February 07, 2012
Export Citation:
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Assignee:
BIONEER CORPORATION (8-11, Munpyeongseo-ro Daedeok-gu, Daejeon 306-220, KR)
PARK, Han Oh (208-601, Expo Apt. Jeonmin-dong, Yuseong-gu, Daejeon 305-761, KR)
YANG, Sung Jun (102-603, Dasarang Apt. Moksang-dong, Daedeok-gu, Daejeon 306-760, KR)
JOO, Sung Mo (1 Samsung Apt, Oryu-dong Jung-gu, Daejeon 301-787, 4-1306, KR)
HWANG, Byoung Oh (1 LG Deokdong Apt, Bijeon 2-don, Pyeongtaek-si Gyeonggi-do 450-729, 05-1501, KR)
International Classes:
C12N9/12; C12N15/54; C12N15/63
Foreign References:
US20020090618A12002-07-11
US20080227661A12008-09-18
US20030003452A12003-01-02
US20070202529A12007-08-30
US6140086A2000-10-31
Other References:
See also references of EP 2673359A2
Attorney, Agent or Firm:
BAE, KIM & LEE IP GROUP (11th Floor, Shinduk Bldg.1329-7 Secho 2-dong, Secho-gu, Seoul 137-858, KR)
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Claims: