Title:
RIBITOL DEHYDROGENASE HAVING DUAL COENZYME SPECIFICITY AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2013/065901
Kind Code:
A1
Abstract:
The present invention relates to noble ribitol dehydrogenase, to a residue determining dual coenzyme specificity, and to a method for producing L-ribulose using the ribitol dehydrogenase. More particularly, the present invention relates to ribitol dehydrogenase producing rare sugars, to nuclear acid molecules coding same, to a vector including the nucleic acid molecules, to a transformant including the vector, to a mutant of the ribitol dehydrogenase, and to a method for producing L-ribulose using the ribitol dehydrogenase. According to the present invention, the ribitol dehydrogenase having dual coenzyme specificity derived from zymomonas mobilis can be effectively used in the production of expensive rare sugars. Also, the demonstration of coenzyme specificity determining factors in the ribitol dehydrogenase is applied to every dehydrogenase as a generic technology.
Inventors:
LEE JUNG KUL (KR)
MOON HEE JUNG (KR)
TIWARI MANISH (KR)
KIM TAE SU (KR)
MOON HEE JUNG (KR)
TIWARI MANISH (KR)
KIM TAE SU (KR)
Application Number:
PCT/KR2012/000623
Publication Date:
May 10, 2013
Filing Date:
January 27, 2012
Export Citation:
Assignee:
UNIV KONKUK IND COOP CORP (KR)
LEE JUNG KUL (KR)
MOON HEE JUNG (KR)
TIWARI MANISH (KR)
KIM TAE SU (KR)
LEE JUNG KUL (KR)
MOON HEE JUNG (KR)
TIWARI MANISH (KR)
KIM TAE SU (KR)
International Classes:
C12N9/02; C12N15/53; C12P19/04
Other References:
MOON, H. J. ET AL.: "Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY., vol. 87, no. 1, 2 February 2010 (2010-02-02), pages 205 - 214, XP019841498
MOON, H. J. ET AL.: "Molecular determinants of the cofactor specificity of ribitol dehydrogenase, a short-chain dehydrogenase/reductase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 78, no. 9, 17 February 2012 (2012-02-17), pages 3079 - 3086, XP055233711, DOI: doi:10.1128/AEM.07751-11
REID, M. F. ET AL.: "Molecular characterization of microbial alcohol dehydrogenases", CRITICAL REVIEWS IN MICROBIOLOGY., vol. 20, no. 1, 1994, pages 13 - 56
DE MUYNCK, C. ET AL.: "Dehydrogenation of ribitol with Gluconobacter oxydans: production and stability of L-ribulose", JOURNAL OF BIOTECHNOLOGY., vol. 125, no. 3, 2 May 2006 (2006-05-02), pages 408 - 415, XP024956664, DOI: doi:10.1016/j.jbiotec.2006.03.017
MOON, H. J. ET AL.: "Molecular determinants of the cofactor specificity of ribitol dehydrogenase, a short-chain dehydrogenase/reductase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 78, no. 9, 17 February 2012 (2012-02-17), pages 3079 - 3086, XP055233711, DOI: doi:10.1128/AEM.07751-11
REID, M. F. ET AL.: "Molecular characterization of microbial alcohol dehydrogenases", CRITICAL REVIEWS IN MICROBIOLOGY., vol. 20, no. 1, 1994, pages 13 - 56
DE MUYNCK, C. ET AL.: "Dehydrogenation of ribitol with Gluconobacter oxydans: production and stability of L-ribulose", JOURNAL OF BIOTECHNOLOGY., vol. 125, no. 3, 2 May 2006 (2006-05-02), pages 408 - 415, XP024956664, DOI: doi:10.1016/j.jbiotec.2006.03.017
Attorney, Agent or Firm:
KOO, Hyun Seo (Daewoo Utopia Bldg., 22, Bangi-dong, Songpa-gu, Seoul 138-050, KR)
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