Title:
RNA CAPPING METHOD, PRODUCTION METHOD FOR MODIFIED RNA, AND MODIFIED RNA
Document Type and Number:
WIPO Patent Application WO/2020/204130
Kind Code:
A1
Abstract:
An RNA capping method that includes a step for reacting RNA and a compound (1) that is represented by general formula (1) in the presence of the Vaccinia virus capping enzyme. (Rb1 represents an oxo group, an alkoxy group, or a halogen atom, Rb2 is absent or represent an alkyl group, Rb3 represents an amino group or a hydrogen atom, Rb4 is absent or represents a hydrogen atom or an alkyl group, Rr1 represents a hydroxy group, a C1–3 alkoxy group, an amino group, an azide group, -OR1C≡CH, or -R2R3, Rr2 represents a hydrogen atom, a hydroxy group, a halogen atom, an alkoxy group, an amino group, an azide group, -OR1C≡CH, or -R2R3, and A1 represents an oxygen atom or a sulfur atom.)
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Inventors:
SAITO HIROHIDE (JP)
OHNO HIROHISA (JP)
AKAMINE SAE (JP)
OHNO HIROHISA (JP)
AKAMINE SAE (JP)
Application Number:
PCT/JP2020/015168
Publication Date:
October 08, 2020
Filing Date:
April 02, 2020
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C07H21/02; C12N15/11; C12P19/34
Foreign References:
| JP2017523777A | 2017-08-24 | |||
| JP2015535430A | 2015-12-14 | |||
| JP2017500034A | 2017-01-05 | |||
| US20170175129A1 | 2017-06-22 | |||
| JP2019073163A | 2019-05-16 |
Other References:
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MAMOT A ET AL.: "Azido-Functionalized 5' Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells", ANGEW CHEM, vol. 56, no. 49, 2017, pages 15628 - 32
WARMINSKI M ET AL.: "Amino-Functionalized 5' Cap Analogs as Tools for Site-Specific Sequence-Independent Labeling of mRNA", BIOCONJUG CHEM, vol. 28, no. 7, 2017, pages 1978 - 92
MUTTACH FL ET AL.: "Chemo-enzymatic modification of eukaryotic mRNA", ORG BIOMOL CHEM, vol. 15, no. 2, 2017, pages 278 - 84
ISSUR M ET AL.: "Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism", PLOS ONE, vol. 8, no. 9, 2013, pages e75310
MARTIN SA ET AL., J BIOL CHEM, vol. 251, no. 23, 1976, pages 7313 - 7321
FUCHS AL ET AL., RNA, vol. 22, no. 9, 2016, pages 1454 - 1466
WESSELHOEFT RA ET AL., NAT COMMUN, vol. 9, no. 1, 6 July 2018 (2018-07-06), pages 2629
GRUDZIEN-NOGALSKA E ET AL.: "Synthetic mRNAs with superior translation and stability properties", METHODS MOL BIOL, vol. 969, 2013, pages 55 - 72
MAMOT A ET AL.: "Azido-Functionalized 5' Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells", ANGEW CHEM, vol. 56, no. 49, 2017, pages 15628 - 32
WARMINSKI M ET AL.: "Amino-Functionalized 5' Cap Analogs as Tools for Site-Specific Sequence-Independent Labeling of mRNA", BIOCONJUG CHEM, vol. 28, no. 7, 2017, pages 1978 - 92
MUTTACH FL ET AL.: "Chemo-enzymatic modification of eukaryotic mRNA", ORG BIOMOL CHEM, vol. 15, no. 2, 2017, pages 278 - 84
ISSUR M ET AL.: "Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism", PLOS ONE, vol. 8, no. 9, 2013, pages e75310
MARTIN SA ET AL., J BIOL CHEM, vol. 251, no. 23, 1976, pages 7313 - 7321
FUCHS AL ET AL., RNA, vol. 22, no. 9, 2016, pages 1454 - 1466
WESSELHOEFT RA ET AL., NAT COMMUN, vol. 9, no. 1, 6 July 2018 (2018-07-06), pages 2629
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
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