Title:
RNA DETECTING KIT AND METHOD
Document Type and Number:
WIPO Patent Application WO/2018/016885
Kind Code:
A1
Abstract:
The present invention relates to a kit and a method for detecting an RNA molecule, particularly, a small-size RNA molecule such as miRNA. The present invention provides an RNA detecting kit and method in which short primers for reverse transcription are used in a reverse transcription process with the aim of rapidly diffusing the primers in a reverse-transcription reaction solution and thus reducing the reverse-transcription reaction time, enabling the overall PCR reaction rate to be increased and RNA to be detected stably and rapidly. In addition, the present invention provides an RNA detecting kit and method in which, after a reverse transcription process, extension is performed on a single-stranded cDNA by use of a primer for extension, far longer than a primer for reverse transcription, and thus a template long enough to conduct PCR amplification can be obtained.
Inventors:
LEE JAE HOON (KR)
Application Number:
PCT/KR2017/007804
Publication Date:
January 25, 2018
Filing Date:
July 20, 2017
Export Citation:
Assignee:
HEIMBIOTEK INC (KR)
International Classes:
C12Q1/68
Foreign References:
| US8383344B2 | 2013-02-26 | |||
| US20140295434A1 | 2014-10-02 | |||
| EP2198059B1 | 2014-12-03 | |||
| US20130045885A1 | 2013-02-21 | |||
| KR20090017670A | 2009-02-18 | |||
| KR20160098097A | 2016-08-18 |
Other References:
See also references of EP 3476945A4
Attorney, Agent or Firm:
LEE, Dong-Ki (KR)
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