Title:
RNA REVERSE TRANSCRIPTION AMPLIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2018/001304
Kind Code:
A1
Abstract:
A ribonucleic acid (RNA) reverse transcription amplification method, comprising the steps of RNA reverse transcription into cDNA and immediate amplification on cDNA, characterized in that the process of RNA reverse transcription into cDNA was completed during the process that a cDNA amplification reaction system is heated to reach the reaction condition for cDNA amplification. The RNA reverse transcription amplification method can combine the reaction condition for RNA reverse transcription into cDNA with the reaction condition for cDNA amplification, thereby significantly shorten the time required for RNA reverse transcription amplification. And in the whole process of RNA reverse transcription amplification, there is no need to change the instrument temperature, thereby it is possible to achieve detection at any time.
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Inventors:
ZHANG SHIYIN (CN)
GE SHENGXIANG (CN)
WANG JIN (CN)
ZHANG JUN (CN)
XIA NINGSHAO (CN)
GE SHENGXIANG (CN)
WANG JIN (CN)
ZHANG JUN (CN)
XIA NINGSHAO (CN)
Application Number:
PCT/CN2017/090759
Publication Date:
January 04, 2018
Filing Date:
June 29, 2017
Export Citation:
Assignee:
UNIV XIAMEN (CN)
International Classes:
C12Q1/68
Foreign References:
CN1552886A | 2004-12-08 | |||
CN103173434A | 2013-06-26 | |||
CN101636505A | 2010-01-27 | |||
CN103173434A | 2013-06-26 | |||
CN1571849A | 2005-01-26 | |||
CN101983236A | 2011-03-02 | |||
CN201110456811A | 2011-12-23 | |||
CN201110360350A | 2011-11-15 |
Other References:
CHOVANCOVA, P. ET AL.: "Reverse-Transcription Quantitative PCR Directly from Cells without RNA Extraction and without Isothermal Reverse-Transcription: a 'Zero-Step' RT-qPCR Protocol", BIOLOGY METHODS AND PROTOCOLS, vol. 2, no. 1, 30 May 2017 (2017-05-30), pages 1 - 6, XP055569729
KRANASTER, R. ET AL.: "Neues Enzym Mit Reverse Transkriptaseund DNA-Polymerase-Funktion", BIOSPEKTRUM, vol. 22, no. 2, 23 March 2016 (2016-03-23), pages 164 - 165, XP035651424
SONG, YANPING ET AL.: "Application Value of HPV E6/E7 mRNA detection in screening of Cervical Cancer", CHINESE JOURNAL OF BIRTH HEALTH & HEREDITY, vol. 24, no. 4, 25 April 2016 (2016-04-25), pages 25 - 27, 39, XP009513050, ISSN: 1006-9534
RAGOZZINO, E. ET AL.: "Development of a One Tube-One Step RT-PCR Protocol for the Detection of Seven Viroids in Four Genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospiviroid.", JOURNAL OF VIROLOGICAL METHODS., vol. 121, 7 July 2004 (2004-07-07), pages 25 - 29, XP004551401
See also references of EP 3498861A4
J. SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
F. M. AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, JOHN WILEY & SONS, INC.
KRANASTER, R. ET AL.: "Neues Enzym Mit Reverse Transkriptaseund DNA-Polymerase-Funktion", BIOSPEKTRUM, vol. 22, no. 2, 23 March 2016 (2016-03-23), pages 164 - 165, XP035651424
SONG, YANPING ET AL.: "Application Value of HPV E6/E7 mRNA detection in screening of Cervical Cancer", CHINESE JOURNAL OF BIRTH HEALTH & HEREDITY, vol. 24, no. 4, 25 April 2016 (2016-04-25), pages 25 - 27, 39, XP009513050, ISSN: 1006-9534
RAGOZZINO, E. ET AL.: "Development of a One Tube-One Step RT-PCR Protocol for the Detection of Seven Viroids in Four Genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospiviroid.", JOURNAL OF VIROLOGICAL METHODS., vol. 121, 7 July 2004 (2004-07-07), pages 25 - 29, XP004551401
See also references of EP 3498861A4
J. SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
F. M. AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, JOHN WILEY & SONS, INC.
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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