(S)-2'-VINYL-ABSCISIC ACID DERIVATIVES

FIELD OF THE INVENTION

[0001] The present invention is directed to a novel class of derivatives of (^-abscisic acid ("(^-ABA") and methods of synthesizing the same.

BACKGROUND OF THE INVENTION

[0002] Abscisic Acid ("ABA") is a naturally occurring plant growth regulator that regulates a wide range of plant physiological processes such as seed germination, seedling elongation, abiotic stress response, flowering, and fruit development. The naturally occurring and most biologically active form of ABA is the S enantiomer (^-ABA. Consequently, a variety of commercial utilities have been identified for (^-ABA in horticulture and agronomy. (^-ABA exerts its biological activities by binding to (^-ABA receptors and activating cellular signal transduction cascades. In addition, (^-ABA has been demonstrated to have pharmaceutical and nutraceutical utilities (see U.S. Patent No. 8,536,224).

[0003] Synthetic derivatives of ABA may exhibit biological activities either similar to

(^-ABA but with altered (enhanced) potency (ABA agonists) or with a differing spectrum of affinity for the multiple ABA receptors than (^-ABA itself. Conversely, synthetic derivatives may act biologically in opposition to (S)-ABA (i.e. as ABA antagonists). The synthetic derivatives may also possess improved uptake by plant tissues as well as enhanced metabolic stability. Additionally, synthetic derivatives may have better chemical and environmental stability than (^-ABA. Thus, synthetic ABA derivatives may possess unique biological activities and have been pursued as an approach to identify novel plant growth regulators.

[0004] A variety of synthetic derivatives of ABA have been known in the public domain.

Several Japanese research groups have synthesized ABA derivatives with modifications of the side chain and/or with cyclohexenone ring substituents through de novo synthesis (Y. Todoroki, at al, Phytochem. 1995, 38, 561-568; Y. Todoroki, et al., Phytochem. 1995, 40, 633-641; S. Nakano, et al, Biosci. Biotech. Biochem. 1995, 59, 1699-176; Y. Todoroki, et al, Biosci. Biotech. Biochem. 1994, 58, 707-715; Y. Todoroki, et al, Biosci. Biotech. Biochem. 1997, 61, 2043-2045; Y. Todoroki, et al, Tetrahedron, 1996, 52, 8081-8098). Synthesis of (_S)-3 '- halogen-ABA, (,S)-3'-azido-ABA and (S)-alkylthio-ABA from (,S)-ABA have also been reported (Y. Todoroki, et al, Tetrahedron, 1995, 51, 6911-6926; S. Arai, et al., Phytochem. 1999, 52, 1185-1193; J. J. Balsevich, et al, Phytochem. 1977, 44, 215-220; Y. Todoroki, et al. Tetrahedron, 2000, 56, 1649-1653; Y. Todoroki, et al, Bioorg. Med. Chem. Lett. 2001, 11, 2381-2384). The work done by S. R. Abrams and coworkers at the Plant Biotechnology Institute at National Research Council of Canada is also noteworthy. Using de novo synthesis approaches, ABA derivatives with modified side-chains or C ₆ ' -substitution have been prepared either as racemic mixtures or, in some cases, as pure stereoisomers {see U.S. Patent No. 5,518,995; D. M. Priest, et al, FEBS Letters, 2005, 579, 4454-4458). A tetralone series of derivatives in which the cyclohexenone ring of (^-ABA is replaced with a bicyclic tetralone ring has also been described (J. M. Nyangulu, et al, Org. Biomol. Chem. 2006, 4, 1400-1412; J. M. Nyangulu, et al, J. Am. Chem. Soc. 2005, 127, 1662-1664; WO2005/108345).

[0005] A few of the examples of (^-ABA synthetic derivatives prepared in the literature are reported to have biological activity as ABA antagonists. A recent example is the work reported by Takeuchi where a series of (,S)-3'-alkylsulfanyl-ABA derivatives were made and tested (Takeuchi, et al, Nature Chem. Biol. 2014, 10, 477-482).

[0006] The synthetic ABA derivatives reported in the literature thus far are limited in scope and are typically prepared via multi-step de novo synthesis. The syntheses generally suffer from low overall yields, particularly when the optically pure single enantiomers are desired. Thus, these compounds are generally expensive to synthesize in large quantities or to manufacture on a commercial scale, limiting their commercial application. The (^-ABA derivatives of the present invention possess the aforementioned biological activities and, more importantly, can be prepared efficiently in single-enantiomer form from (^-ABA, which until recently was not available in large quantities.

[0007] Accordingly, there is a need for entantiomerically pure (^-ABA derivatives which are agonists and/or antagonists of (^-ABA with improved or oppositional biological activity, respectively. There is also a need for improved (^-ABA derivative synthesis methods. SUMMARY OF THE INVENTION

[0008] In one aspect, the invention is directed to single enantiomer compounds of

Formula I:

wherein R is selected from the group consisting of a substituted or unsubstituted benzene, naphthylene, monocyclic or bicyclic heterocyclic aromatic ring, and salts thereof.

[0009] In another aspect, the invention is directed to a process for making the compounds of the present invention.

[00010] In a further aspect, the invention is directed to methods for regulating plant growth comprising applying an effective amount of at least one of the compounds of the present invention to a plant in need of growth regulation.

DETAILED DESCRIPTION OF THE INVENTION

[00011] Applicant has discovered new (^-ABA derivatives which are enantiomerically pure and methods for synthesizing these (,S)-ABA derivatives. The compounds of the present invention are (^-ABA derivatives that are relatively easy to synthesize.

[00012] In one embodiment, the present invention is directed to enantiomerically pure compounds of Formula I:

wherein R is selected from the group consisting of a substituted or unsubstituted benzene, naphthylene, a monocyclic or bicyclic heterocyclic aromatic ring, and salts thereof. [00013] In an embodiment, R is unsubstituted benzene.

[00014] In a preferred embodiment, R is a substituted benzene.

[00015] In a more preferred embodiment, the benzene ring can be mono-, di-, tri-, tetra- or penta-substituted.

[00016] In another embodiment, the benzene ring substitution is selected from the group consisting of halogen, substituted or unsubstituted alkyl, cyano, alkoxy, nitro, sulfonyl, carbonyl, carboalkoxy and carbamoyl.

[00017] In a preferred embodiment, the benzene ring substitution is an unsubstituted lower alkyl. In a more preferred embodiment, the unsubstituted lower alkyl is methyl. In a most preferred embodiment, the methyl substitution is 4-methyl-.

[00018] In yet another preferred embodiment, the benzene ring is mono-substituted with one halogen. In a more preferred embodiment, the halogen is fluorine or chlorine.

[00019] In a further preferred embodiment, the benzene ring halogen substitution is 4- chloro.

[00020] In a further embodiment, the benzene ring is disubstituted with two halogen substitutions, including difluoro-, dichloro-, and chloro-fluoro-.

[00021] In a further preferred embodiment, the benzene ring dihalogen substitutions are 3- chloro-4-fluoro-.

[00022] In an alternative preferred embodiment, the benzene ring dihalogen substitutions are 3,4-difluoro.

[00023] In yet another embodiment, the benzene ring is mono-substituted with dihalomethyl or trihalomethyl. In a preferred embodiment, the benzene ring substitution is trihalomethyl. In a more preferred embodiment, the trihalomethyl is trichloromethyl or trifluoromethyl. In a most preferred embodiment, the benzene ring substitution is 4- trifluoromethyl.

[00024] In another embodiment, the benzene ring is disubstituted with at least one halogen and at least one dihalomethyl or trihalomethyl. In a preferred embodiment, the benzene ring substitutions are a halogen and a trihalomethyl. In a more preferred embodiment, the halogen is fluoro and the trihalomethyl is trifluoromethyl. In a most preferred embodiment the fluoro is 2- fluoro and the trifluoromethyl is 3 -trifluoromethyl.

[00025] In another embodiment, R is a mono-cyclic or bicyclic heterocyclic aromatic ring.

[00026] In a preferred embodiment, the heterocyclic aromatic ring is selected from the group consisting of pyridine, furan, thiazole, and salts thereof.

[00027] In a more preferred embodiment, R is a pyridyl group. In a further preferred embodiment, the pyridyl is selected from the group consisting of 2-pyridyl, 3-pyridyl and 4- pyridyl.

[00028] In another preferred embodiment, R is a furyl group. In a further preferred embodiment, the furyl group is a 2-furyl group.

[00029] In yet another preferred embodiment, R is a thiazolyl group. In a further preferred embodiment, the thiazolyl is selected from the group consisting of 2-thiazolyl and 3-thiazolyl.

[00030] In an alternative embodiment, the present invention is directed to a process for making the compounds of Formula I. This process includes (Step a) reacting (S)-abscisic acid with an alkylating agent R'X and a base in a solvent, (Step b) treating the compound resulting from Step a with a base and an aryl or heteroaryl aldehyde in a solvent, and (Step c) treating the compound resulting from Step b with a base, water and an organic solvent. This synthesis is illustrated in Scheme I below.

Scheme I:

[00031] Suitable alkylating agents for use in Step a include methyl iodide and methyl sulfate (R'=methyl), ethyl iodide, (R'=ethyl) and benzyl bromide (R'=benzyl).

[00032] Suitable bases for use in Step a include potassium carbonate, sodium carbonate, cesium carbonate, sodium hydroxide and potassium hydroxide.

[00033] Suitable solvents for use in Step a include acetone, N, N-dimethylformamide, N- methylpyrolidone and dimethylsulfoxide.

[00034] Suitable bases for use in Step b include lithium bis(trimethylsilyl)amide and sodium hydride.

[00035] Suitable solvents for use in Step a include tetrahydrofuran and N, N- dimethylformamide.

[00036] Suitable bases for use in Step c include lithium hydroxide, sodium hydroxide and potassium hydroxide.

[00037] Suitable organic solvents for use in Step c include methanol, ethanol and isopropanol.

[00038] In another embodiment, the present invention is directed to methods for regulating plant growth comprising applying an effective amount of at least one of the compounds of the present invention to a plant in need of growth regulation.

[00039] The compounds of the present invention have a wide range of utilities, including promoting germination in plant species with high seed dormancy, promoting and synchronizing bud break in woody perennial plant species, promoting plant growth, promoting plant heat stress tolerance, and inhibiting leaf yellowing, flower drop, and leaf drop in ornamental plant species. Additionally, these compounds may have utility in the nutraceutical and pharmaceutical areas.

[00040] The disclosed embodiments are simply exemplary embodiments of the inventive concepts disclosed herein and should not be considered as limiting, unless the claims expressly state otherwise. Definitions of Terms

[00041] As used herein, "vinyl" refers to the functional group -CH=CH ₂ , which is an ethylene molecule (H ₂ C=CH ₂ ) minus one hydrogen atom.

[00042] As used herein, "alkenyl" refers to the functional group -CR'=CR"R"', which is an substituted ethylene molecule (R'HC=CR'R"') minus one hydrogen atom.

[00043] As used herein, "alkyl" refers to a saturated straight or branched chain alkane radical (i.e. a group missing one of the hydrogen atoms that would be required for a stable molecule) and containing at least one carbon (-C _n H _2n+1 ). Examples of alkyls include methyl, ethyl, propyl, z ^' so-propyl, butyl, sec-butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, and dodecyl. As used herein, "lower alkyl" refers to an alkyl with 6 or fewer carbons.

[00044] As used herein, "substituted alkyl" refers to a straight or branched chain alkane radical that contains at least one carbon and one of the hydrogens of the core structure has been replaced. Examples of substituted alkyls include hydroxymethyl (-CH ₂ OH), hydroxybutyl (- C ₄ ¾-OH), trifluoromethyl (-CF ₃ ), pentafluoroethyl (-CF ₂ CF ₃ ).

[00045] As used herein, "substituted compound" is one in which one or more hydrogen atoms of a core structure have been replaced with a functional group such as alkyl, hydroxy, or halogen.

[00046] As used herein, "substituted benzene ring" refers to a benzene ring wherein one or more hydrogen atoms of a core structure have been replaced with a functional group, such as an alkyl or a halogen.

[00047] As used herein, "pyridine" refers to a six-membered ring heterocyclic aromatic compound with the chemical formula C ₅ H ₅ N. It is structurally similar to benzene but it has one methine group (=CH-) replaced by a nitrogen atom.

[00048] As used herein, "furan" refers to a five membered heterocyclic aromatic compound with the chemical formula C ₄ H ₄ 0.

[00049] As used herein, "thiazole" refers to five membered heterocyclic aromatic compound that contains a sulfur and a nitrogen atom.

[00050] As used herein, "cyano" refers to a radical with the formula -C≡N. [00051] As used herein, "halo-" refers to fluoro-, chloro-, bromo- or iodo-. Embodiments of the present invention include di and trihalogens.

[00052] As used herein, "dihalomethyl" refers to a functional group with two halogens replacing the hydrogens of methyl. For example, -CHF ₂ .

[00053] As used herein, "trihalomethyl" refers to a functional group with three halogens replacing the hydrogens of methyl. For example, -CF ₃ .

[00054] As used herein, "enantiomerically pure" or "(5) " refer to the presence of a single enantiomer of ABA with the relative enantiomeric purity of greater than 95%.

[00055] As used herein, "racemic" or "(±)" refer to a relatively equal mixture of R/S enantiomers.

[00056] As used herein "salts" refers to those salts which retain the biological effectiveness and properties of the parent compounds and which are not biologically or otherwise harmful at the dosage administered. Salts of the compounds of the present inventions may be prepared from inorganic or organic acids or bases, such as Na ⁺ or K ⁺ salts derived from neutralization of an ABA carboxylic acid group with sodium hydroxide or potassium hydroxide or RiR ₂ R ₃ R ₄ N ⁺ salts prepared by neutralization with an amine. Amine salts include but are not limited to the ammonium salt prepared by neutralization with ammonium hydroxide or the triethylammonium salt prepared by neutralization with triethylamine.

[00057] As used herein, all numerical values relating to amounts, weight percentages and the like are defined as "about" or "approximately" each particular value, namely, plus or minus 10% (± 10%)). For example, the phrase "at least 5% by weight" is to be understood as "at least 4.5%) to 5.5%) by weight." Therefore, amounts within 10% of the claimed values are encompassed by the scope of the claims.

[00058] The articles "a," "an" and "the" are intended to include the plural as well as the singular, unless the context clearly indicates otherwise.

[00059] The following examples are intended to illustrate the present invention and to teach one of ordinary skill in the art how to use the formulations of the invention. They are not intended to be limiting in any way. EXAMPLES

Example 1 Compound la

(2Z,4E)-methyl 5-((,S)-l-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-l-yl)-3 -methylpenta-2,4- dienoate

[00060] A solution of (S)-ABA (53 g, 0.2 mole) in acetone (800 ml) was cooled with an ice bath. Cesium carbonate (98 g, 0.3 mole) was added. The mixture was stirred for ten minutes, then methyl iodide (24.8 ml, 56.5 g, 0.4 mole) was added. After stirring at ambient temperature overnight, the mixture was concentrated to -300 ml and water (500 ml) was added. The resulting mixture was then extracted with ethyl acetate (3x200 ml). The resulting organic solution was washed twice with saturated aqueous sodium sulfite solution, dried (MgS0 ₄ ) and filtered. Evaporation of the filtrate gave the title compound as an off-white solid (56 g).

Compound lb

(2Z,4E)-methyl 5-((5)-2-((E)-2-chloro-5-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[00061] A solution of Compound la (0.56 g, 2.0 mmole) in anhydrous tetrahydrofuran

(THF, 10 mL) was cooled to 0°C with an ice bath under an atmosphere of nitrogen. Lithium hexamethyl disilazane (1.0 M solution in THF, 3.0 mL) was added dropwise via a syringe over 10 minutes. The resulting solution was stirred at 0°C for 30 minutes and the ice bath was removed. A solution of 2-chloro-5-trifluoromethylbenzaldehyde (2.08 g, 10 mmole) in anhydrous THF (4 mL) was then added via a syringe over 5 minutes. The resulting solution was stirred at ambient temperature overnight. The reaction was then quenched with water (20 mL) and acidified to ~pH 2.0 with 1.0 N aqueous HCl. The mixture was extracted with ethyl acetate (2x20 mL). The combined organic solution was dried (MgS0 ₄ ), filtered, and concentrated. The residue was purified on a silica gel column eluted with ethyl acetate and hexane to give the desired product (0.384 g).

Compound 1

(2Z,4E)-5-((5)-2-((E)-2-chloro-5-(trifluoromethyl)styryl) -l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[00062] To a solution of Compound lb (0.38 g, 0.82 mmole) in methanol (15 mL) and water (2 mL) was added lithium hydroxide mono-hydrate (0.4 g, 10 mmole). The mixture was stirred at room temperature overnight and evaporated to remove most of the methanol. Water (10 ml) was added. The resulting mixture was cooled with an ice bath and acidified with 6 N aqueous HCl to pH 2-3, resulting in a white precipitation. The mixture was extracted with ethyl acetate (3x20 ml). The combined organic solution was dried (MgS0 ₄ ), filtered and evaporated. The residue was purified on a silica gel column eluting with a gradient of ethyl acetate and hexane containing 1% acetic acid. The title compound was obtained as a white solid (0.278 g). ¹ HNMR (CDC1 ₃ ): 67.91 (d, 1H), 7.81 (s, 1H), 7.60 (d, 1H), 7.45 (m, 2H), 6.89 (d, 1H), 6.42 (s, 1H), 6.26 (d, 1H), 5.77 (s, 1H), 2.58 (d, 1H), 2.38 (d, 1H), 2.07 (s, 3H), 1.18 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=453. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 2 Compound 2a

(2Z,4E)-methyl 5-((,S)-2-((E)-4-fluorostyryl)-l-riydroxy-6,6-dimetriyl-4-ox ocyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00063] The title compound was prepared according to the procedure of Compound lb, substituting 4-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 2

(2Z,4E)-5-((5)-2-((E)-4-fluorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid [00064] The title compound was prepared according to the procedure of Compound 1, substituting Compound 2a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.92 (d, 1H), 7.43 (m, 2H), 7.18 (d, 1H), 7.03 (m, 2H), 6.77 (d, 1H), 6.34 (s, 1H), 6.26 (d, 1H), 5.77 (s, 1H), 2.56 (d, 1H), 2.36 (d, 1H), 2.11 (s, 3H), 1.15 (s, 3H), 1.07 (s, 3H). MS (ESI-): m/e=369. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 3

Compound 3 a

(2Z,4E)-methyl 5-((,S)-2-((E)-4-cyanostyryl)-l-hydroxy-6,6-dimethyl-4-oxocy clohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00065] The title compound was prepared according to the procedure of Compound lb, substituting 4-cyanobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 3

(2Z,4E)-5-((S)-2-((E)-4-cyanostyryl)- 1 -hydroxy-6,6-dimethyl-4-oxocyclohex-2-en- 1 -yl)-3 - methylpenta-2,4-dienoic acid [00066] The title compound was prepared according to the procedure of Compound 1, substituting Compound 3a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, 1H), 7.58 (s, 1H), 7.52 (s, 1H), 7.19 (d, 1H), 6.97 (d, 1H), 6.37 (s, 1H), 6.27 (d, 1H), 5.79 (s, 1H), 2.59 (d, 1H), 2.38 (d, 1H), 2.08 (s, 3H), 1.16 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=376. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 4

Compound 4a

(2Z,4E)-methyl 5-((,S)-l-hydroxy-6,6-dimethyl-2-((E)-4-methylstyryl)-4-oxoc yclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[00067] The title compound was prepared according to the procedure of Compound lb, substituting 4-methylbenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 4

(2Z,4E)-5-((S)-l-hydroxy-6,6-dimethyl-2-((E)-4-methylstyryl) -4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00068] The title compound was prepared according to the procedure of Compound 1, substituting Compound 4a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.92 (d, 1H), 7.35 (d, 1H), 7.18-7.11 (m, 4H), 6.80 (d, 1H), 6.35 (s, 1H), 6.25 (d, 1H), 5.76 (s, 1H), 2.55 (d, 1H), 2.32 (d, 1H), 2.30 (s, 3H), 2.04 (s, 3H), 1.15 (s, 3H), 1.06 (s, 3H). MS (ESI-): m/e=365. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 5 Compound 5 a

(2Z,4E)-methyl 5-((,S)-2-((E)-2,6-dichlorostyryl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[00069] The title compound was prepared according to the procedure of Compound lb, substituting 2, 6-dichlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 5

(2Z,4E)-5-((5)-2-((E)-2,6-dichlorostyryl)- 1 -hydroxy-6,6-dimethyl-4-oxocyclohex-2-en- 1 -yl)-3 - methylpenta-2,4-dienoic acid

[00070] The title compound was prepared according to the procedure of Compound 1, substituting Compound 5a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, 1H), 7.28 (d, 2H), 7.20 (d, 1H), 7.08 (t, 1H), 6.91 (d, 1H), 6.39 (s, 1H), 6.27 (d, 1H), 5.75 (s, 1H), 2.56 (d, 1H), 2.41 (d, 1H), 2.06 (d, 3H), 1.18 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 6

Compound 6a

(2Z,4E)-methyl 5-((,S)-2-((E)-2,6-difluorostyryl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[00071] The title compound was prepared according to the procedure of Compound lb, substituting 2, 6-difluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 6

(2Z,4E)-5-((5)-2-((E)-2,6-difluorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00072] The title compound was prepared according to the procedure of

Compound 1, substituting Compound 6a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, 1H), 7.23 (d, 1H), 7.21-7.15 (m, 2H), 6.85 (t, 2H), 6.39 (s, 1H), 6.27 (d, 1H), 5.75 (s, 1H), 2.56 (d, 1H), 2.39 (d, 1H), 2.06 (d, 3H), 1.16 (s, 3H), 1.07 (s, 3H). MS (ESI-): m/e=387. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 7

Compound 7a

(2Z,4E)-methyl 5-((,S)-2-((E)-2-chloro-6-fluorostyryl)-l-hydroxy-6,6-dimeth yl-4-oxocyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate

[00073] The title compound was prepared according to the procedure of Compound lb, substituting 2-chloro-6-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde. Compound 7

(2Z,4E)-5-((,S)-2-((E)-2-chloro-6-fluorostyryl)-l-hydroxy -6,6-dimethyl-4-oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid

[00074] The title compound was prepared according to the procedure of Compound 1, substituting Compound 7a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.92 (d, 1H), 7.37 (d, 1H), 7.20-7.11 (m, 2H), 7.09 (d, 1H), 7.00-6.90 (m, 1H), 6.39 (s, 1H), 6.28 (d, 1H), 5.76 (s, 1H), 2.57 (d, 1H), 2.40 (d, 1H), 2.06 (d, 3H), 1.17 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=403. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 8

Compound 8a

(2Z,4E)-methyl 5-((,S)-2-((E)-3-chloro-4-fluorostyryl)-l-hydroxy-6,6-dimeth yl-4-oxocyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate [00075] The title compound was prepared according to the procedure of Compound lb, substituting 3-chloro-4-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 8

(2Z,4E)-5-((,S)-2-((E)-3-chloro-4-fluorostyryl)-l-hydroxy -6,6-dimethyl-4-oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid

[00076] The title compound was prepared according to the procedure of Compound 1, substituting Compound 8a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.11 (br s, 1H), 7.77 (d, 1H), 7.73 (dd, 1H), 7.54-7.50 (m, 1H), 7.41 (t, 1H), 7.34 (d, 1H), 6.92 (d, 1H), 6.30 (s, 1H), 6.30 (d, 1H), 5.67 (s, 1H), 5.50 (s, 1H), 2.58 (d, 1H), 2.14 (d, 1H), 1.96 (d, 3H), 0.99 (s, 3H), 0.95 (s, 3H). MS (ESI-): m/e=403. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 9

Compound 9a

(2Z,4E)-methyl 5-((,S)-2-((E)-4-chloro-3-fluorostyryl)-l-hydroxy-6,6-dimeth yl-4-oxocyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate

[00077] The title compound was prepared according to the procedure of Compound lb, substituting 4-chloro-3-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 9

(2Z,4E)-5-((,S)-2-((E)-4-chloro-3-fluorostyryl)-l-hydroxy -6,6-dimethyl-4-oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid

[00078] The title compound was prepared according to the procedure of Compound 1, substituting Compound 9a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.1 (br s, 1H), 7.79 (d, 1H), 7.59 (t, 1H), 7.56 (dd, 1H), 7.38 (d, 1H), 7.37 (d, 1H), 6.99 (d, 1H), 6.32 (s, 1H), 6.32 (d, 1H), 5.68 (s, 1H), 5.51 (s, 1H), 2.60 (d, 1H), 2.16 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=403. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 10

Compound 10a

(2Z,4E)-methyl 5-((,S)-2-((E)-2-fluorostyryl)-l-hydroxy-6,6-dimethyl-4-oxoc yclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00079] The title compound was prepared according to the procedure of Compound lb, substituting 2-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 10

(2Z,4E)-5-((5)-2-((E)-2-fluorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00080] The title compound was prepared according to the procedure of Compound 1, substituting Compound 10a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, 1H), 7.52 (dt, 1H), 7.37 (d, 1H), 7.26-7.19 (m, 1H), 7.08 (dt, 1H), 7.01 (ddd, 1H), 6.93 (d, 1H), 6.39 (s, 1H), 6.26 (d, 1H), 5.76 (s, 1H), 2.58 (d, 1H), 2.37 (d, 1H), 2.30 (s, 3H), 2.06 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=369. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure. Example 11

Compound 11a

(2Z,4E)-methyl 5-((5)-2-((E)-3-cyano-4-methoxystyryl)-l-hydroxy-6,6-dimethy l-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[00081] The title compound was prepared according to the procedure of Compound lb, substituting 2-fluoro-5-formylbenzonitrile for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 11

(2Z,4E)-5-((,S)-2-((E)-3-cyano-4-methoxystyryl)-l-hydroxy -6,6-dimethyl-4-oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid

[00082] The title compound was prepared according to the procedure of Compound 1, substituting Compound 11a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, 1H), 7.66 (d, 1H), 7.59 (dd, 1H) 7.13 (d, 1H), 6.91 (d, 1H), 6.79 (d, 1H), 6.36 (s, 1H), 6.25 (d, 1H), 5.77 (s, 1H), 3.92 (s, 3H), 2.58 (d, 1H), 2.35 (d, 1H), 2.07 (d, 3H), 1.16 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=406. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 12

Compound 12a

(2Z,4E)-methyl 5-((,S)-2-((E)-2-chlorostyryl)-l-riydroxy-6,6-dimetriyl-4-ox ocyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00083] The title compound was prepared according to the procedure of Compound lb, substituting 2-chlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 12

(2Z,4E)-5-((5)-2-((E)-2-chlorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00084] The title compound was prepared according to the procedure of Compound 1, substituting Compound 12a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.92 (d, 1H), 7.63 (d, 1H), 7.61-7.58 (m, 1H), 7.34-7.31 (m, 1H), 7.22-7.15 (m, 2H), 6.83 (d, 1H), 6.41 (s, 1H), 6.27 (d, 1H), 5.76 (s, 1H), 2.58 (d, 1H), 2.38 (d, 1H), 2.06 (d, 3H), 1.17 (s, 3H), 1.07 (s, 3H). MS (ESI-): m/e=385. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 13

Compound 13a

(2Z,4E)-methyl 5-((5)-2-((E)-2-chloro-3-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[00085] The title compound was prepared according to the procedure of Compound lb, substituting 2-chloro-3-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 13

(2Z,4E)-5-((5)-2-((E)-2-chloro-3-(trifluoromethyl)styryl)-l- hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid [00086] The title compound was prepared according to the procedure of Compound 1, substituting Compound 13a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, IH), 7.76 (dd, IH), 7.68 (d, IH), 7.60 (dd, IH), 7.31 (t, IH), 6.84 (d, IH), 6.41 (s, IH), 6.27 (d, IH), 5.77 (s, IH), 2.59 (d, IH), 2.38 (d, IH), 2.07 (d, 3H), 1.18 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=453. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 14

Compound 14a

(2Z,4E)-methyl 5-((5)-2-((E)-4-chloro-3-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[00087] The title compound was prepared according to the procedure of Compound lb, substituting 4-chloro-3-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 14

(2Z,4E)-5-((5)-2-((E)-4-chloro-3-(trifluoromethyl)styryl) -l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[00088] The title compound was prepared according to the procedure of Compound 1, substituting Compound 14a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.89 (d, 1H), 7.71 (d, 1H), 7.53 (dd, 1H), 7.42 (d, 1H), 7.19 (d, 1H), 6.90 (d, 1H), 6.37 (s, 1H), 6.25 (d, 1H), 5.77 (s, 1H), 2.57 (d, 1H), 2.36 (d, 1H), 2.07 (d, 3H), 1.16 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=453. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 15

Compound 15a

(2Z,4E)-methyl 5-((,S)-2-((E)-2,4-dichlorostyryl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate [00089] The title compound was prepared according to the procedure of Compound lb, substituting 2,4-dichlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 15

(2Z,4E)-5-((5)-2-((E)-2,4-dichlorostyryl)- 1 -hydroxy-6,6-dimethyl-4-oxocyclohex-2-en- 1 -yl)-3 - methylpenta-2,4-dienoic acid

[00090] The title compound was prepared according to the procedure of Compound 1, substituting Compound 15a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, 1H), 7.54 (d, 1H), 7.52 (d, 1H), 7.33 (d, 1H), 7.17 (dd, 1H), 6.82 (d, 1H), 6.39 (s, 1H), 6.27 (d, 1H), 5.77 (s, 1H), 2.58 (d, 1H), 2.37 (d, 1H), 2.07 (d, 3H), 1.17 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 16

Compound 16a

(2Z,4E)-methyl 5-((,S)-2-((E)-3,5-dichlorostyryl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[00091] The title compound was prepared according to the procedure of Compound lb, substituting 3,5-dichlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 16

(2Z,4E)-5-((5)-2-((E)-3,5-dichlorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00092] The title compound was prepared according to the procedure of Compound 1, substituting Compound 16a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.88 (d, 1H), 7.29 (d, 1H), 7.22 (t, 1H), 7.07 (d, 1H), 6.86 (d, 1H), 6.35 (s, 1H), 6.23 (d, 1H), 5.77 (s, 1H), 2.57 (d, 1H), 2.35 (d, 1H), 2.06 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 17

Compound 17a

(2Z,4E)-methyl 5-((,S)-2-((E)-3-chlorostyryl)-l-hydroxy-6,6-dimethyl-4-oxoc yclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00093] The title compound was prepared according to the procedure of Compound lb, substituting 3-chlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 17

(2Z,4E)-5-((5)-2-((E)-3-chlorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00094] The title compound was prepared according to the procedure of Compound 1, substituting Compound 17a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 67.80 (d, 1H), 7.59 (t, 1H), 7.49 (dt, 1H), 7.40 (d, 1H), 7.38-7.34 (m, 2H), 6.99 (d, 1H), 6.34 (s, 1H), 6.32 (d, 1H), 5.68 (s, 1H), 5.52 (s, 1H), 2.59 (d, 1H), 2.17 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=385. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 18

Compound 18a

(2Z,4E)-methyl 5-((,S)-2-((E)-4-chlorostyryl)-l-riydroxy-6,6-dimetriyl-4-ox ocyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00095] The title compound was prepared according to the procedure of Compound lb, substituting 4-chlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 18

(2Z,4E)-5-((5)-2-((E)-4-chlorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[00096] The title compound was prepared according to the procedure of Compound 1, substituting Compound 18a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.89 (d, 1H), 7.35 (d, 2H), 7.25 (d, 2H), 7.14 (d, 1H), 6.82 (d, 1H), 6.35 (s, 1H), 6.22 (d, 1H), 5.75 (s, 1H), 2.56 (d, 1H), 2.36 (d, 1H), 2.03 (d, 3H), 1.14 (s, 3H), 1.06 (s, 3H). MS (ESI-): m/e=385. Two-dimensional MR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 19

Compound 19a

(2Z,4E)-methyl 5-((,S)-2-((E)-3,4-dichlorostyryl)-l-riydroxy-6,6-dimetriyl- 4-oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[00097] The title compound was prepared according to the procedure of Compound lb, substituting 3,4-dichlorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 19

(2Z,4E)-5-((5)-2-((E)-3,4-dichlorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid [00098] The title compound was prepared according to the procedure of Compound 1, substituting Compound 19a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 67.79 (d, 1H), 7.78 (d, 1H), 7.64 (d, 1H), 7.52 (dd, 1H), 7.37 (d, 1H), 7.01 (d, 1H), 6.34 (s, 1H), 6.32 (d, 1H), 5.69 (s, 1H), 5.53 (s, 1H), 2.60 (d, 1H), 2.17 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=419. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 20

Compound 20a

(2Z,4E)-methyl 5-((,S)-2-((E)-3-fluorostyryl)-l-hydroxy-6,6-dimethyl-4-oxoc yclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[00099] The title compound was prepared according to the procedure of Compound lb, substituting 3-fluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 20

(2Z,4E)-5-((5)-2-((E)-3-fluorostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid [000100] The title compound was prepared according to the procedure of Compound 1, substituting Compound 20a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.10 (br s, 1H), 7.81 (d, 1H), 7.46-7.32 (m, 4H), 7.14 (t, 1H), 6.97 (d, 1H), 6.33 (s, 1H), 6.32 (d, 1H), 5.68 (s, 1H), 5.51 (s, 1H), 2.60 (d, 1H), 2.17 (d, 1H), 1.98 (s, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=369. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 21

Compound 21a

(2Z,4E)-methyl 5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-3-(trifluorometh yl)styryl)cyclohex-

2-en- 1 -yl)-3 -methylpenta-2,4-dienoate

[000101] The title compound was prepared according to the procedure of Compound lb, substituting 3-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 21

(2Z,4E)-5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-3-(trifl uoromethyl)styiyl)cyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid

[000102] The title compound was prepared according to the procedure of Compound 1, substituting Compound 21a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, 1H), 7.66 (s, 1H), 7.61 (d, 1H), 7.49 (d, 1H), 7.41 (t, 1H), 7.23 (d, 1H), 6.92 (d, 1H), 6.39 (s, 1H), 6.25 (d, 1H), 5.76 (s, 1H), 2.58 (d, 1H), 2.36 (d, 1H), 2.06 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 22

Compound 22a

(2Z,4E)-methyl 5-((S)-2-((E)-2,4-difluorostyryl)- 1 -hydroxy-6,6-dimethyl-4-oxocyclohex-2-en- 1 - yl)-3-methylpenta-2,4-dienoate

[000103] The title compound was prepared according to the procedure of Compound lb, substituting 2,4-difluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde. Compound 22

(2Z,4E)-5-((5)-2-((E)-2,4-difluorostyryl)-l-hydroxy-6,6-dime thyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000104] The title compound was prepared according to the procedure of Compound 1, substituting Compound 22a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.06 (br s, 1H), 7.80 (d, 1H), 7.71 (dt, 1H), 7.35 (d, 1H), 7.32-7.25 (m, 1H), 7.13 (dt, 1H), 6.97 (d, 1H), 6.32 (d, 1H), 6.31 (s, 1H), 5.68 (s, 1H), 5.51 (s, 1H), 2.58 (d, 1H), 2.19 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.96 (s, 3H). MS (ESI-): m/e=387. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 23

Compound 23 a

(2Z,4E)-methyl 5-((S)-2-((E)-3 ,4-difluorostyryl)- 1 -hydroxy-6,6-dimethyl-4-oxocyclohex-2-en- 1 - yl)-3-methylpenta-2,4-dienoate [000105] The title compound was prepared according to the procedure of Compound lb, substituting 3,4-difluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 23

(2Z,4E)-5-((5)-2-((E)-3,4-difluorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000106] The title compound was prepared according to the procedure of Compound 1, substituting Compound 23a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.10 (br s, 1H), 7.79 (d, 1H), 7.58 (ddd, 1H), 7.47-7.34 (m, 2H), 7.35 (d, 1H), 6.92 (d, 1H), 6.31 (d, 1H), 6.30 (s, 1H), 5.68 (s, 1H), 5.50 (s, 1H), 2.59 (d, 1H), 2.16 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=387. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 24

Compound 24a

(2Z,4E)-methyl 5-((,S)-2-((E)-3,5-difluorostyryl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoate

[000107] The title compound was prepared according to the procedure of Compound lb, substituting 3,5-difluorobenzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 24

(2Z,4E)-5-((5)-2-((E)-3,5-difluorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000108] The title compound was prepared according to the procedure of Compound 1, substituting Compound 24a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.13 (br s, 1H), 7.80 (d, 1H), 7.37 (d, 1H), 7.24 (dd, 2H), 7.16 (tt, 1H), 7.02 (d, 1H), 6.32 (d, 1H), 6.32 (s, 1H), 5.69 (s, 1H), 5.52 (s, 1H), 2.61 (d, 1H), 2.17 (d, 1H), 1.99 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=387. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 25 Compound 25a

(2Z,4E)-methyl 5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(trifluorometh yl)styryl)cyclohex-

2-en- 1 -yl)-3 -methylpenta-2,4-dienoate

[000109] The title compound was prepared according to the procedure of Compound lb, substituting 2-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 25

(2Z,4E)-5-((5)-2-((E)-3,5-difluorostyryl)-l-hydroxy-6,6-d imethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000110] The title compound was prepared according to the procedure of Compound 1, substituting Compound 25a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, IH), 7.68 (d, IH), 7.61-7.53 (m, 2H), 7.47 (t, IH), 7.33 (t, IH), 6.80 (d, IH), 6.35 (s, IH), 6.27 (d, IH), 5.76 (s, IH), 2.58 (d, IH), 2.37 (d, IH), 2.06 (d, 3H), 1.17 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 26 Compound 26a

(2Z,4E)-methyl 5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-4-(trifluorometh yl)styryl)cyclohex-

2-en- 1 -yl)-3 -methylpenta-2,4-dienoate 00111] The title compound was prepared according to the procedure of Compound lb, substituting 4-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 26

(2Z,4E)-5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-4-(tr ifluoromethyl)styryl)cyclohex-2-en-l- yl)-3-methylpenta-2,4-dienoic acid [000112] The title compound was prepared according to the procedure of Compound 1, substituting Compound 26a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.93 (d, 1H), 7.52 (m, 4H), 7.22 (d, 1H), 6.96 (d, 1H), 6.39 (s, 1H), 6.27 (d, 1H), 5.77 (s, 1H), 2.59 (d, 1H), 2.37 (d, 1H), 2.07 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=419. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 27

Compound 27a

(2Z,4E)-methyl 5-((5)-2-((E)-2-fluoro-5-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[000113] The title compound was prepared according to the procedure of Compound lb, substituting 2-fluoro-5-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 27

(2Z,4E)-5 (^-2 (E)-2-fluoro-5-(trifluoromethyl)styryl)-l-hydroxy-6,6-dimeth yl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[000114] The title compound was prepared according to the procedure of Compound 1, substituting Compound 27a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, IH), 7.78 (dd, IH), 7.51-7.46 (m, IH), 7.34 (d, IH), 7.12 (t, IH), 6.99 (d, IH), 6.40 (s, IH), 6.25 (d, IH), 5.77 (s, IH), 2.58 (d, IH), 2.37 (d, IH), 2.07 (d, 3H), 1.17 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=327 (fragment + HCOO ^" ). Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 28

Compound 28

(2Z,4E)-5-((5)-l-hydroxy-2-((E)-2-methoxy-5-(trifluoromet hyl)styryl)-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[000115] The title compound was isolated as one of the products from the preparation of

Compound 27 from Compound 27a. ¹ HNMR (CDC1 ₃ ): 67.92 (d, IH), 7.72 (d, IH), 7.53 (d, IH), 7.48 (dd, IH), 6.93 (d, IH), 6.89 (d, IH), 6.41 (s, IH), 6.26 (d, IH), 5.76 (s, IH), 3.87 (s, 3H), 2.57 (d, IH), 2.38 (d, IH), 2.06 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=449. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure. Example 29 Compound 29a

(2Z,4E)-methyl 5-((5)-2-((E)-3-fluoro-5-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[000116] The title compound was prepared according to the procedure of Compound lb, substituting 3-fluoro-5-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 29

(2Z,4E)-5-((5)-2-((E)-3-fluoro-5-(trifluoromethyl)styryl) -l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[000117] The title compound was prepared according to the procedure of Compound 1, substituting Compound 29a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.88 (d, 1H), 7.46 (s, 1H), 7.33 (dt, 1H), 7.20 (dt, 1H), 7.19 (d, 1H), 6.92 (d, 1H), 6.38 (s, 1H), 6.24 (d, 1H), 5.77 (s, 1H), 2.57 (d, 1H), 2.36 (d, 1H), 2.06 (d, 3H), 1.16 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=437. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 30

Compound 30a

(2Z,4E)-methyl 5-((5)-2-((E)-2-fluoro-4-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[000118] The title compound was prepared according to the procedure of Compound lb, substituting 2-fluoro-4-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 30

(2Z,4E)-5-((5)-2-((E)-2-fluoro-4-(trifluoromethyl)styryl) -l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid [000119] The title compound was prepared according to the procedure of Compound 1, substituting Compound 30a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.92 (d, IH), 7.63 (t, IH), 7.34 (d, IH), 7.34 (d, IH), 7.26 (d, IH), 7.03 (d, IH), 6.40 (s, IH), 6.27 (d, IH), 5.77 (s, IH), 2.59 (d, IH), 2.38 (d, IH), 2.07 (d, 3H), 1.17 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=437. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 31

Compound 31a

(2Z,4E)-methyl 5-((5)-2-((E)-2-fluoro-3-(trifluoromethyl)styryl)-l-hydroxy- 6,6-dimethyl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoate

[000120] The title compound was prepared according to the procedure of Compound lb, substituting 2-fluoro-3-(trifluoromethyl)benzaldehyde for 2-chloro-5-trifluoromethyl- benzaldehyde.

Compound 31

(2Z,4E)-5 (^-2 (E)-2-fluoro-3-(trifluoromethyl)styryl)-l-hydroxy-6,6-dimeth yl-4- oxocyclohex-2-en-l-yl)-3-methylpenta-2,4-dienoic acid

[000121] The title compound was prepared according to the procedure of Compound 1, substituting Compound 31a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.90 (d, 1H), 7.72 (t, 1H), 7.49 (t, 1H), 7.36 (d, 1H), 7.18 (d, 1H), 6.98 (d, 1H), 6.40 (s, 1H), 6.26 (d, 1H), 5.77 (s, 1H), 2.59 (d, 1H), 2.38 (d, 1H), 2.07 (d, 3H), 1.17 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=437. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 32

Compound 32a

(2Z,4E)-methyl 5-((,S)-2-((E)-3-cyanostyryl)-l-hydroxy-6,6-dimethyl-4-oxocy clohex-2-en-l-yl)-

3-methylpenta-2,4-dienoate

[000122] The title compound was prepared according to the procedure of Compound lb, substituting 3-formylbenzonitrile for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 32

(2Z,4E)-5-((,S)-2-((E)-3-cyanostyryl)-l-hydroxy-6,6-dimet hyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000123] The title compound was prepared according to the procedure of Compound 1, substituting Compound 32a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, IH), 7.74 (s, IH), 7.65 (dt, IH), 7.51 (dt, IH), 7.41 (t, IH), 7.19 (d, IH), 6.94 (dd, IH), 6.38 (s, IH), 6.25 (d, IH), 5.78 (s, IH), 2.58 (d, IH), 2.37 (d, IH), 2.06 (d, 3H), 1.17 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=376. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 33

Compound 33a

(2Z,4E)-methyl 5-((5)-2-((E)-2-(furan-2-yl)vinyl)-l-hydroxy-6,6-dimethyl-4- oxocyclohex-2-en- l-yl)-3-methylpenta-2,4-dienoate [000124] The title compound was prepared according to the procedure of Compound lb, substituting furfural for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 33

(2Z,4E)-5-((5)-2-((E)-2-(furan-2-yl)vinyl)-l-hydroxy-6,6- dimethyl-4-oxocyclohex-2-en-l-yl)-3- methylpenta-2,4-dienoic acid

[000125] The title compound was prepared according to the procedure of Compound 1, substituting Compound 33a for Compound lb. ¹ HNMR (CDC1 ₃ ): 67.91 (d, IH), 7.43 (s, IH), 7.10 (d, IH), 6.75 (d, IH), 6.47 (d, IH), 6.42 (t, IH), 6.32 (s, IH), 6.26 (d, IH), 5.77 (s, IH), 2.56 (d, IH), 2.36 (d, IH), 2.07 (s, 3H), 1.17 (s, 3H), 1.08 (s, 3H). MS (ESI-): m/e=341. Two- dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 34

Compound 34a

(2Z,4E)-methyl 5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(thiazol-2-yl)v inyl)cyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate [000126] The title compound was prepared according to the procedure of Compound lb, substituting 2-thiazolecarboxaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 34

(2Z,4E)-5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(thi azol-2-yl)vinyl)cyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoic acid

[000127] The title compound was prepared according to the procedure of Compound 1, substituting Compound 34a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.05 (br s, IH), 7.90 (d, IH), 7.81 (d, IH), 7.77 (d, IH), 7.57 (d, IH), 7.11 (d, IH), 6.43 (s, IH), 6.32 (d, IH), 5.69 (s, IH), 5.59 (s, IH), 2.60 (d, IH), 2.20 (d, IH), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=358. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 35

Compound 35a

(2Z,4E)-methyl 5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(thiazol-5-yl)v inyl)cyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate [000128] The title compound was prepared according to the procedure of Compound lb, substituting 5-thiazolecarboxaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 35

(2Z,4E)-5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(thi azol-5-yl)vinyl)cyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoic acid

[000129] The title compound was prepared according to the procedure of Compound 1, substituting Compound 35a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.11 (br s, 1H), 9.06 (s, 1H), 7.90 (s, 1H), 7.79 (d, 1H), 7.68 (d, 1H), 6.69 (d, 1H), 6.32 (s, 1H), 6.31 (d, 1H), 5.68 (s, 1H), 5.54 (s, 1H), 2.60 (d, 1H), 2.17 (d, 1H), 1.98 (d, 3H), 1.00 (s, 3H), 0.96 (s, 3H). MS (ESI-): m/e=358. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 36

Compound 36a

(2Z,4E)-methyl 5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyridin-2-yl)v inyl)cyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate

[000130] The title compound was prepared according to the procedure of Compound lb, substituting 2-pyridinecarboxaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 36

(2Z,4E)-5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyr idin-2-yl)vinyl)cyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoic acid

[000131] The title compound was prepared according to the procedure of Compound 1, substituting Compound 36a for Compound lb. ¹ HNMR (CDC1 ₃ ): 68.53 (d, IH), 8.16 (d, IH), 7.71 (dt, IH), 7.52 (d, IH), 7.36 (d, IH), 7.27 (d, IH), 7.23 (dd, IH), 6.39 (s, IH), 6.23 (d, IH), 5.77 (s, IH), 2.53-2.40 (d, IH), 2.01 (d, 3H), 1.17 (s, 3H), 1.09 (s, 3H). MS (ESI-): m/e=352. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 37

Compound 37a

(2Z,4E)-methyl 5-((,S)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyridin-3-yl) vinyl)cyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate

[000132] The title compound was prepared according to the procedure of Compound lb, substituting 3-pyridinecarboxaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 37

(2Z,4E)-5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyr idin-3-yl)vinyl)cyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoic acid

[000133] The title compound was prepared according to the procedure of Compound 1, substituting Compound 37a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 612.09 (br s, lh), 8.69 (s, IH), 8.49 (d, IH), 7.94 (dt, IH), 7.80 (d, IH), 7.40 (d, IH), 7.40 (dd, IH), 7.03 (d, IH), 6.34 (s, IH), 6.33 (d, IH), 5.68 (s, IH), 5.50 (s, IH), 2.60 (d, IH), 2.17 (d, IH), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=352. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 38 Compound 38a

(2Z,4E)-methyl 5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyridin-4-yl)v inyl)cyclohex-2- en- 1 -yl)-3 -methylpenta-2,4-dienoate

[000134] The title compound was prepared according to the procedure of Compound lb, substituting 4-pyridinecarboxaldehyde for 2-chloro-5-trifluoromethylbenzaldehyde.

Compound 38

(2Z,4E)-5-((5)-l-hydroxy-6,6-dimethyl-4-oxo-2-((E)-2-(pyr idin-4-yl)vinyl)cyclohex-2-en-l-yl)-

3-methylpenta-2,4-dienoic acid

[000135] The title compound was prepared according to the procedure of Compound 1, substituting Compound 38a for Compound lb. ¹ HNMR (DMSO-d ₆ ): 68.55 (d, 2H), 7.80 (d, IH), 7.45 (dd, 2H), 7.35 (d, IH), 7.16 (d, IH), 6.37 (s, IH), 6.33 (d, IH), 5.68 (s, IH), 5.55 (br s, 1H), 2.62 (d, 1H), 2.18 (d, 1H), 1.98 (d, 3H), 1.01 (s, 3H), 0.97 (s, 3H). MS (ESI-): m/e=352. Two-dimensional NMR experiments (COSY, NOESY, HSQC and HMBC) confirmed the structure.

Example 39

Seed germination assay for testing Compounds 1 to 38

[000136] To determine whether the (S)-ABA derivatives of the present invention were effective antagonists, germination assays were performed with the model plant Arabidopsis thaliana. If the compounds are found to be antagonists, they may be useful to counter any undesirable side effects of (^-ABA.

[000137] Arabidopsis seed was sterilized by shaking for five minutes in 200 proof ethanol, followed by shaking for five minutes in a 10% bleach solution. The seeds were then washed five times in sterile, distilled, de-ionized water and suspended in 0.1% phytoagar. The tubes containing the seeds were wrapped in aluminum foil and stratified at 4°C for two days.

[000138] All compounds were tested at a concentration of 30 ppm in the presence or absence of 0.3 ppm (^-ABA, in 24-well plates. For each compound, a 1000 ppm stock solution was prepared in distilled, deionized water containing 5% DMSO. The stock test solution (15 xL) was added to each well, plus or minus 1.5 [iL of 100 ppm (^-ABA in 5% DMSO. Appropriate amounts of distilled, de-ionized water was added to equalize volumes, and 450 μΙ_, of ½ x Murashige and Skoog media containing 1.2% Bactoagar was then added to each well (final DMSO concentration was 0.5%). When the media solidified, ten to 15 sterile stratified Arabidopsis seeds were distributed into each well using a repeat pipettor. The plates were sealed with surgical tape and placed in a growth chamber running diurnal cycles of 12 hours of light at 24°C and 12 hours of darkness at 19°C. The plates were scanned on days 3 through 14, and scored on day 14. The (5)-2' -vinyl-substituted ABA derivatives were scored on ability to promote germination in the presence of (S)-ABA. The results are summarized below in "Table 1 : Arabidopsis seed germination promotion activity of 2'-vinyl-(S)-ABA derivatives." Table 1 : Arabidopsis seed germination promotion activity of 2'-vinyl-(,S)-ABA derivatives

- : Seeds germinated at approximately the same time as those treated with 0.3 ppm of (S)-ABA alone (i.e., treatment with the 2'-vinyl-(,S)-ABA derivative showed no measurable effect in this assay configuration).

+ : Seeds germinated 1 to 4 days earlier than those treated with 0.3 ppm of (^-ABA alone.

++ : Seeds germinated 5 to 7 days earlier than those treated with 0.3 ppm of (^-ABA alone.

+++ : Seeds germinated 8 to 10 days earlier than those treated with 0.3 ppm of (S)- ABA alone.

[000139] This biological assay is indicative of the overall antagonist nature of the derivatives compared to (^-ABA.

[000140] In this assay, Applicant unexpectedly found that Compounds 4, 8, 18, 23, 26 and

31 were antagonists of (^-ABA. Based on the known functions of (,S)-AB A in plant physiology, these unexpected results imply that Compounds 4, 8, 18, 23, 26 and 31 will be effective at countering (,S)-AB A action, including undesirable physiological effects of (,S)-AB A treatment.

Example 40

Field study for breaking grape vine dormancy

[000141] A small plot field study to test the effect of experimental compounds on release of, or breaking grape vine dormancy was conducted near Thermal, California in January, 2016. The grapevines, Seedless 'Red Flame', had been pruned to 2-4 node spurs (shortened canes) prior to treatment. Aqueous treatment solutions were prepared with a test compound, an appropriate buffering agent, and a silicone-based nonionic surfactant at 0.25% (v/v). Each spur was thoroughly wetted with a 1% (w/v) solution, which was applied with a foam brush. Dormancy breaking was assessed periodically by examining bud break (initiation of growth) and subsequent shoot development. Compound 23, one of the most potent (S)-ABA antagonist as shown in Example 39 was chosen for this study. Both a control treatment (aqueous solution with surfactant) and a positive control treatment (the commercial standard for bud breaking, hydrogen cyanamide) were included in this study for comparison.

Table 2. Effect of Compound 23 on grape bud break and shoot development.

[000142] The application of compound 23 to dormant grape spurs significantly increased bud break.. Unexpectedly, it was observed that at the 31 day post treatment assessment, treatment with compound 23 resulted in a significantly higher percentage of buds broken in comparison with the control (16.7% vs. 9.2%). Furthermore, it was unexpectedly observed that at 48 day post-treatment, treatment with compound 23 resulted in a higher percentage of broken buds than either the commercial standard or control (76.7% vs. 58.8% and 61.7%, respectively). The shoot length/spur from the broken buds for the vines treated with compound 23 was intermediate between the spurs treated with the control and hydrogen cyanamide. These results indicate that compound 23 is active in promoting breaking of woody plant dormancy.

[000143] Thus, these novel compounds as (^-ABA antagonists, are effective for promoting germination in plant species with high seed dormancy and for promoting and synchronizing bud break in woody perennial plant species. These (S)-ABA antagonists may possess other agronomically valuable biological activities such as promoting plant growth, promoting plant heat stress tolerance, and inhibiting leaf yellowing, flower drop, and leaf drop in ornamental plant species.