Title:
(S)-SELECTIVE HYPERACTIVE MUTANT ARYLMALONATE DECARBOXYLASE
Document Type and Number:
WIPO Patent Application WO/2012/117999
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a mutant arylmalonate decarboxylase of (S)-selective hyperactive form which is capable of producing (S)-α-arylpropionic acid with an optical purity of almost 100%e.e. from arylmalonic acid. The mutant arylmalonate decarboxylase of (S)-selective hyperactive form, which is capable of producing (S)-α-arylpropionic acid from arylmalonic acid, has mutation (i), mutations (i) and (ii), mutations (i) and (iii), or mutations (i), (ii) and (iii) in the amino acid sequence of wild-type arylmalonate decarboxylase that is represented by SEQ ID NO:2:
(i) substitution of glycine at 74 position by cysteine, and substitution of cysteine at 188 position by glycine;
(ii) substitution of tyrosine at 48 position by another amino acid; and
(iii) substitution of methionine at 159 position by another amino acid.
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Inventors:
MIYAMOTO KENJI (JP)
Application Number:
PCT/JP2012/054731
Publication Date:
September 07, 2012
Filing Date:
February 27, 2012
Export Citation:
Assignee:
UNIV KEIO (JP)
MIYAMOTO KENJI (JP)
MIYAMOTO KENJI (JP)
International Classes:
C12N15/09; C12N9/88; C12P7/40
Domestic Patent References:
| WO2009068308A2 | 2009-06-04 |
Foreign References:
| JP2003169685A | 2003-06-17 |
Other References:
MIYAUCHI, Y. ET AL.: "Dramatically improved catalytic activity of an artificial (S)- selective arylmalonate decarboxylase by structure-guided directed evolution", CHEMICAL COMMUNICATIONS, vol. 47, no. 26, 14 July 2011 (2011-07-14), pages 7503 - 7505
YUSUKE MIYAUCHI ET AL.: "Arylmalon San Datsu Tansan Koso no Kino Kaihen", THE 14TH SYMPOSIUM ON BIOCATALYST CHEMISTRY JAPAN IN SHIZUOKA, KOEN YOSHISHU, 23 September 2010 (2010-09-23), pages 28 - 29
OBATA, R. ET AL.: "Structural Basis for Inverting the Enantioselectivity of Arylmalonate Decarboxylase Revealed by the Structural Analysis of the Gly74Cys/Cysl88Ser Mutant in the Liganded Form", BIOCHEMISTRY, vol. 49, 2010, pages 1963 - 1969
KENJI MIYAMOTO: "Arylmalon San Datsu Tansan Koso no Kino Kaihen", KOSO KOGAKU NEWS, vol. 10, no. 64, 2010, pages 5 - 10
KENJI MIYAMOTO ET AL.: "Hen'i 1 ko de Kotonaru Kino o Fuyo, Hen'i 2 ko de Koso no Enantio Sentakusei Gyakuten", BIOSCIENCE & INDUSTRY, vol. 63, no. 9, 2005, pages 583 - 586
TERAO Y. ET AL.: "Improvement of the activity of arylmalonate decarboxylase by random mutagenesis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 73, 2006, pages 647 - 653
YUSUKE MIYAUCHI ET AL.: "Arylmalon San Datsu Tansan Koso no Kino Kaihen", THE 14TH SYMPOSIUM ON BIOCATALYST CHEMISTRY JAPAN IN SHIZUOKA, KOEN YOSHISHU, 23 September 2010 (2010-09-23), pages 28 - 29
OBATA, R. ET AL.: "Structural Basis for Inverting the Enantioselectivity of Arylmalonate Decarboxylase Revealed by the Structural Analysis of the Gly74Cys/Cysl88Ser Mutant in the Liganded Form", BIOCHEMISTRY, vol. 49, 2010, pages 1963 - 1969
KENJI MIYAMOTO: "Arylmalon San Datsu Tansan Koso no Kino Kaihen", KOSO KOGAKU NEWS, vol. 10, no. 64, 2010, pages 5 - 10
KENJI MIYAMOTO ET AL.: "Hen'i 1 ko de Kotonaru Kino o Fuyo, Hen'i 2 ko de Koso no Enantio Sentakusei Gyakuten", BIOSCIENCE & INDUSTRY, vol. 63, no. 9, 2005, pages 583 - 586
TERAO Y. ET AL.: "Improvement of the activity of arylmalonate decarboxylase by random mutagenesis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 73, 2006, pages 647 - 653
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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