Title:
SACCHAROMYCES CEREVISIAE REGULATORY ELEMENT AND USE THEREOF IN CAROTENOID SYNTHESIS
Document Type and Number:
WIPO Patent Application WO/2021/259383
Kind Code:
A1
Abstract:
Provided are a regulatory element having a nucleotide sequence as shown in SEQ ID NO:1 that antagonizes the genomic position effect of Saccharomyces cerevisiae, and the use thereof. Chromosomes near the gal80 site of Saccharomyces cerevisiae have special structures, and may have other types of position effects that are non-HM site (mating type allocation site) silencing effects. The transcription activation region of TEF2 is added to two ends of a synthetic pathway with a gene sequence of crtE-crtB-crtI, which can antagonize the unknown position effect of the gal80 site and significantly increase the content of lycopene. The antagonistic effect mainly prevents the influence of the position effect on gene transcription in the synthetic pathway.
Inventors:
ZHU HONGHUI (CN)
SU BULI (CN)
WANG DONGDONG (CN)
LI ANZHANG (CN)
DENG MINGRONG (CN)
FENG GUANGDA (CN)
SU BULI (CN)
WANG DONGDONG (CN)
LI ANZHANG (CN)
DENG MINGRONG (CN)
FENG GUANGDA (CN)
Application Number:
PCT/CN2021/108448
Publication Date:
December 30, 2021
Filing Date:
July 26, 2021
Export Citation:
Assignee:
INST OF MICROBIOLOGY GUANGDONG ACADEMY OF SCIENCES GUANGDONG DETECTION CENTER OF MICROBIOLOGY (CN)
International Classes:
C12N1/19; C12N15/113; C12N15/53; C12N15/54; C12N15/81; C12N15/90; C12P5/02; C12R1/865
Foreign References:
| CN112574993A | 2021-03-30 | |||
| CN112063646A | 2020-12-11 | |||
| CN102199624A | 2011-09-28 | |||
| CN109136119A | 2019-01-04 | |||
| US20130115656A1 | 2013-05-09 |
Other References:
WU XIAO-LE, LI BING-ZHI, ZHANG WEN-ZHENG, SONG KAI, QI HAO, DAI JUN-BIAO, YUAN YING-JIN: "Genome-wide landscape of position effects on heterogeneous gene expression in Saccharomyces cerevisiae", BIOTECHNOLOGY FOR BIOFUELS, vol. 10, no. 1, 1 December 2017 (2017-12-01), pages 189, XP055882421, DOI: 10.1186/s13068-017-0872-3
ZHANG WENZHENG, TANG JIJUN, LI BINGZHI, YUAN YINGJIN: "Effect of integration loci of genome on heterologous gene expression in Saccharomyces cerevisiae", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 32, no. 7, 25 July 2016 (2016-07-25), CN , pages 901 - 911, XP055882423, ISSN: 1000-3061, DOI: 10.13345/j.cjb.150442
ZHANG WENZHENG, TANG JIJUN, LI BINGZHI, YUAN YINGJIN: "Effect of integration loci of genome on heterologous gene expression in Saccharomyces cerevisiae", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 32, no. 7, 25 July 2016 (2016-07-25), CN , pages 901 - 911, XP055882423, ISSN: 1000-3061, DOI: 10.13345/j.cjb.150442
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (CN)
Download PDF:
Previous Patent: TIMING METHOD AND SYSTEM FOR SHARED PRODUCTS
Next Patent: CULTURE MEANS FOR SYNTHESIZING SACCHAROMYCES CEREVISIAE CAROTENOID
Next Patent: CULTURE MEANS FOR SYNTHESIZING SACCHAROMYCES CEREVISIAE CAROTENOID