Title:
SALIVA ASSAY METHOD AND DEVICE
Document Type and Number:
WIPO Patent Application WO/1995/027205
Kind Code:
A1
Abstract:
An improved method for determining the presence of an analyte in an oral fluid sample. In the method, a reaction mixture is produced containing at least a portion of the collected sample and a chromogenic substrate effective to produce a colored product upon reaction with amylase present in the sample. After a selected reaction time, the reaction medium is inspected to determine the level of colored product produced in the medium within the selected reaction time. Production of colored product above a preselected threshold level within the selected reaction time indicates that an effective volume of undiluted saliva sample has been collected sufficient to allow detection of the analyte. The invention also includes a device for use in the method.
Inventors:
THIEME THOMAS R (US)
CIMLER B MELINA (US)
KLIMKOW NANETTE M (US)
CIMLER B MELINA (US)
KLIMKOW NANETTE M (US)
Application Number:
PCT/US1995/003809
Publication Date:
October 12, 1995
Filing Date:
March 29, 1995
Export Citation:
Assignee:
EPITOPE INC (US)
THIEME THOMAS R (US)
CIMLER B MELINA (US)
KLIMKOW NANETTE M (US)
THIEME THOMAS R (US)
CIMLER B MELINA (US)
KLIMKOW NANETTE M (US)
International Classes:
G01N33/52; G01N33/53; G01N33/558; A61B10/00; (IPC1-7): G01N33/52; G01N33/53; C12Q1/40
Domestic Patent References:
| WO1993011434A1 | 1993-06-10 |
Foreign References:
| EP0083941A1 | 1983-07-20 | |||
| US2850903A | 1958-09-09 | |||
| EP0520408A2 | 1992-12-30 |
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