Title:
SALMONELLA AND SHEET-TYPE M CELLS
Document Type and Number:
WIPO Patent Application WO/2015/025638
Kind Code:
A1
Abstract:
[Problem] To provide a detoxified salmonella capable of potentiating a protein that is required for function enhancement in M cells (microfold cells), sheet-type M cells wherein the protein required for function enhancement of M cells has been potentiated by the detoxified salmonella, and an artificial micro organ into which the sheet-type M cells has been introduced.
[Solution] A salmonella capable of invading into M cells, said salmonella being detoxified Salmonella (Ty21a) derived, via mutation, from Salmonella (Ty2). This detoxified Salmonella (Ty21a) further carries a gene producing galectin-9 that is capable of enhancing the function of M cells, said gene having been introduced by genetic recombination. Therefore, galectin-9 in the M cells is thus potentiated and, in its turn, enhances the function of the M cells. The detoxified Salmonella (Ty21a) having invaded into the M cells disappears with the passage of time.
Inventors:
FUKUNAGA HAJIME (JP)
EZAKI TAKAYUKI (JP)
EZAKI TAKAYUKI (JP)
Application Number:
PCT/JP2014/068671
Publication Date:
February 26, 2015
Filing Date:
July 14, 2014
Export Citation:
Assignee:
K & I INC (JP)
International Classes:
C12N1/21; C12N5/07; C12N15/09
Domestic Patent References:
| WO2012077811A1 | 2012-06-14 |
Foreign References:
| JPH05197880A | 1993-08-06 | |||
| JP2001501831A | 2001-02-13 |
Other References:
TATSUO YAMAMOTO: "Bacterial vaccine development", CLINICAL MICROBIOLOGY, 20 November 1993 (1993-11-20), pages 783 - 792
YU , B. ET AL.: "A method to generate recombinant Salmonella typhi Ty21a strains expressing multiple heterologous genes using an improved recombineering strategy", APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 177 - 188
OSORIO, M. ET AL.: "Anthrax Protective Antigen Delivered by Salmonella enterica Serovar Typhi Ty21a Protects Mice from a Lethal Anthrax Spore Challenge", INFECTION AND IMMUNITY, vol. 7, no. 4, 7 April 2009 (2009-04-07), pages 1475 - 1482
YU , B. ET AL.: "A method to generate recombinant Salmonella typhi Ty21a strains expressing multiple heterologous genes using an improved recombineering strategy", APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 177 - 188
OSORIO, M. ET AL.: "Anthrax Protective Antigen Delivered by Salmonella enterica Serovar Typhi Ty21a Protects Mice from a Lethal Anthrax Spore Challenge", INFECTION AND IMMUNITY, vol. 7, no. 4, 7 April 2009 (2009-04-07), pages 1475 - 1482
Attorney, Agent or Firm:
KOYAMA, YUU (JP)
Hill 有 (JP)
Hill 有 (JP)
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