Title:
SARINGOSTEROL SYNTHETASE, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/035469
Kind Code:
A1
Abstract:
The present invention relates to the field of biotechnology, in particular to a saringosterol synthetase, and a preparation method therefor and the use thereof. The saringosterol synthetase of the present invention can be used to efficiently synthesize saringosterol by means of a one-step enzymatic reaction. This way is completely different from other known hydroxylase-dependent SHP synthetic routes, such as enzyme-mediated oxidation or an autooxidation in-vitro saringosterol synthetic route. The method for synthesizing saringosterol by using saringosterol synthetase in the present invention lays a foundation for preparing side-chain-hydroxylated phytosterol by means of a green enzymology method, metabolic engineering method and synthetic biology means, and has important practical significance for the treatment of atherosclerosis, diabetes, Alzheimer's disease and tumors.
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Inventors:
LU YANDU (CN)
ZHOU WENXU (CN)
ZHANG XU (CN)
GAN QINHUA (CN)
ZHOU WENXU (CN)
ZHANG XU (CN)
GAN QINHUA (CN)
Application Number:
PCT/CN2021/138252
Publication Date:
March 16, 2023
Filing Date:
December 15, 2021
Export Citation:
Assignee:
UNIV HAINAN (CN)
International Classes:
C12N9/00; C12N15/52; C12N15/70; C12N15/81; C12P33/06
Foreign References:
| CN113604442A | 2021-11-05 | |||
| US9487762B1 | 2016-11-08 |
Other References:
DATABASE Genbank NCBI; . . : "uncharacterized protein CHLRE_12g500500v5 [Chlamydomonas reinhardtii]", XP093045555
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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