Title:
SCHIZOCHYTRIUM LIMACINUM STRAIN, BUILDING METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/205165
Kind Code:
A1
Abstract:
Provided is a Schizochytrium limacinum strain, a building method therefor and an application thereof. The strain disclosed is classified and named as Schizochytrium sp. HX-RS, and the preservation number is CCTCC NO: M2017046. An acyltransferase functional domain originating from shewanella PKS enzyme is adopted instead of an acyltransferase functional domain originating from schizochytrium sp. PKS enzyme, and the strain is obtained by performing flat panel screening and acclimation screening with a high rotation speed and a low temperature.
Inventors:
HUANG HE (CN)
REN LUJING (CN)
CHEN SHENGLAN (CN)
GENG LINGJUN (CN)
JI XIAOJUN (CN)
HU XUECHAO (CN)
REN LUJING (CN)
CHEN SHENGLAN (CN)
GENG LINGJUN (CN)
JI XIAOJUN (CN)
HU XUECHAO (CN)
Application Number:
PCT/CN2017/083764
Publication Date:
November 15, 2018
Filing Date:
May 10, 2017
Export Citation:
Assignee:
UNIV NANJING TECHNOLOGY (CN)
International Classes:
C12N1/15; C12P7/64; C12R1/645
Foreign References:
| CN104974944A | 2015-10-14 | |||
| CN102741267A | 2012-10-17 |
Other References:
LI, . HUILING ET AL.: "A High-DHA Production Strain of Schizochytrium sp. Through Mutagenesis", FOOD SCIENCE AND TECHNOLOGY, vol. 40, no. 9, 31 December 2015 (2015-12-31), ISSN: 1005-9989
ZHUANG, XIAOYAN ET AL.: "Establishment of Genetic Transformation System of Schizochytrium sp.by Homologous Recombination", ACTA MICROBIOLOGICA SINICA, vol. 55, no. 4, 4 April 2015 (2015-04-04), ISSN: 0001-6209
ZHAO, . XIAOWEI ET AL.: "Thraustochytrids and Their Biosyntheses of Docosahexaenoic Acid", JOURNAL OF WENZHOU NORMAL COLLEGE, vol. 25, no. 5, 30 October 2004 (2004-10-30), ISSN: 1006-0375
FENG, YUN ET AL.: "Effects of Culture Media on Docosahexaenoic Acid Production by Schizochytrium sp", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, vol. 11, no. 5, 30 September 2013 (2013-09-30), ISSN: 1672-3678
ZHUANG, XIAOYAN ET AL.: "Establishment of Genetic Transformation System of Schizochytrium sp.by Homologous Recombination", ACTA MICROBIOLOGICA SINICA, vol. 55, no. 4, 4 April 2015 (2015-04-04), ISSN: 0001-6209
ZHAO, . XIAOWEI ET AL.: "Thraustochytrids and Their Biosyntheses of Docosahexaenoic Acid", JOURNAL OF WENZHOU NORMAL COLLEGE, vol. 25, no. 5, 30 October 2004 (2004-10-30), ISSN: 1006-0375
FENG, YUN ET AL.: "Effects of Culture Media on Docosahexaenoic Acid Production by Schizochytrium sp", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, vol. 11, no. 5, 30 September 2013 (2013-09-30), ISSN: 1672-3678
Attorney, Agent or Firm:
JIANGSU CO-FAR LAW FIRM (CN)
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