received by the International Bureau on 20 January 2020 (20.01.2020)

WE CLAIM:

1. A kit for screening different grades of cervical cancer comprising of: a) Gold Nanoparticle (AuNPs) having size in the range of 40-50 nm as the SERS substrate;

b) Preservative fluid;

c) Density gradient solution;

d) Pho sphate B uffered S aline (PBS); and

e) Pre- coated glass slide.

2. The kit as claimed in claim 1, wherein the concentration of the nanoparticle is in the range of 8-10 x 10 particles/L.

3. The kit as claimed in claim 1, wherein the preservative fluid used for fixing the sample on the slide is selected from the group comprising of > 50% Ethanol, Methanol, Isopropanol, Formaldehyde, Saline solution, Di potassium hydrogen phosphate.

4. The kit as claimed in claim 1, where in the density gradient solution is 20-50% (w/v) of sucrose in MilliQ water.

5. The kit as claimed in claim 1, wherein the phosphate buffered saline is composed by Sodium Chloride, Potassium Chloride, Disodium phosphate, Potassium dihydrogen phosphate.

6. The kit as claimed in claim 1, wherein the glass slide is pre-coated with compound selected from the group comprising of Poly-L-lysine, APES [(3- Aminopropyl) triethoxy silane.

7. The kit as claimed in claim 1, wherein the different grades of cervical cancer is selected from the group consisting of NRML (Normal), HSIL (High-grade Squamous Intraepithelial Lesion), CSCC (Cervical Squamous Cell Carcinoma).

8. A method for detection of different grades of cervical cancer comprising the following steps: a. providing cell samples in preservative fluid comprising of > 50% Ethanol, Methanol, Isopropanol, Formaldehyde, Saline solution, Di- potassium hydrogen phosphate;

17 b. centrifuging the cell samples obtained in step a to obtain a pellet by using density gradient fluid comprising of 20-50% (w/v) of sucrose in MilliQ water;

c. resuspending the cell pellet obtained in step b in PBS buffer comprising of Sodium Chloride, Potassium Chloride, Disodium phosphate, Potassium dihydrogen phosphate;

d. providing glass slide pre-coated with compounds comprising of Poly-L- lysine, APES [(3- Aminopropyl) triethoxy silane;

e. dropping down the cell suspension obtained in step C onto pre-coated glass slide obtained in step d;

f. incubating the dropped down cell suspension onto pre-coated glass slide obtained in step e with AuNPs for 10-30 minutes; and

g. measuring the surface enhanced Raman scattering from the cell samples and analyzing the Raman spectral pattern to differentiate the three grades of cervical cancer i.e. NRML, HSIL and CSCC.

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