Title:
SCREENING METHOD FOR DISCOVERING AUXILIARY AGENTS PROMOTING ENDOCYTOSIS
Document Type and Number:
WIPO Patent Application WO2002016935
Kind Code:
A9
Abstract:
The invention relates to a screening method for discovering auxiliary agents promoting the endocytosis of low molecular and/or macro-molecular compounds, especially nucleic acids, in cells. The inventive method comprises the following steps: a) a yeast strain is selected and yeast cells are cultivated therefrom; b) the yeast cells are incubated with a potential auxiliary agent promoting the endocytosis of low molecular and/or macro-molecular compounds, especially nucleic acids, in cells in addition to a nucleic acid which is replicable and/or replicable in the nucleus and/or in the cytosol of the yeast cells or a low molecular and/or macro-molecular compound, c) the transformation rates or the transport rates into the cytosol or the nucleus are determined and analysis occurs if the transformation rates or transport rates increase in comparison with the transformation rates and transport rates in a reference test in which the auxiliary substance is not used, d) the auxiliary substance is selected or rejected according to the result of the value obtained for the increase in the transformation rates or transport rates or transport rates in step c). The invention also relates to a screening method of the above-mentioned variety, wherein vertebrate cells are used, in addition to the use of auxiliary agents obtained according to said method in order to produce pharmaceutical substances.
Inventors:
LANG CHRISTINE (DE)
GESSNER REINHARD (DE)
NEUKAMM BIRGIT (DE)
PRINZ BIANKA (DE)
NEVOIGT ELKE (DE)
GESSNER REINHARD (DE)
NEUKAMM BIRGIT (DE)
PRINZ BIANKA (DE)
NEVOIGT ELKE (DE)
Application Number:
PCT/EP2001/007509
Publication Date:
September 04, 2003
Filing Date:
June 30, 2001
Export Citation:
Assignee:
RINA NETZWERK RNA TECHNOLOGIEN (DE)
LANG CHRISTINE (DE)
GESSNER REINHARD (DE)
NEUKAMM BIRGIT (DE)
PRINZ BIANKA (DE)
NEVOIGT ELKE (DE)
LANG CHRISTINE (DE)
GESSNER REINHARD (DE)
NEUKAMM BIRGIT (DE)
PRINZ BIANKA (DE)
NEVOIGT ELKE (DE)
International Classes:
C12Q1/02; G01N33/50; (IPC1-7): G01N33/50; C12Q1/02
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